2025/05/27 更新

写真a

ホソイ　ショウコ
細井　祥子
HOSOI Shoko
所属
環境科学研究院
部局
環境科学部 環境生態学科
職名
准教授
URL
http://des-usp.com/staff/laboratory-of-environmental-microbiology.php
外部リンク

学歴

  • 京都大学   農学研究科   応用生物科学

    　 詳細を見る

    国名： 日本国

  • 京都大学   農学研究科   応用生物科学

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    国名： 日本国

  • 京都大学

  • 京都大学

  • 筑波大学   生物資源学部

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    国名： 日本国

  • 筑波大学

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学位

  • 博士（農学） （ 2003年3月   京都大学 ）

研究キーワード

  • 環境微生物学、分子微生物学 有毒プランクトン　赤潮　貝毒　琵琶湖固有種

  • 環境学

経歴

  • 滋賀県立大学   環境科学部 環境生態学科   准教授

    2014年10月 - 現在

  • 滋賀県立大学   環境科学部 環境生態学科   助教

    2012年9月 - 2014年9月

  • 神戸大学内海域環境教育研究センター   助教

    2005年11月 - 2012年8月

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    国名：日本国

  • 日本学術振興会特別研究員   日本学術振興会特別研究員

    2004年4月 - 2005年10月

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    国名：日本国

  • 滋賀県琵琶湖研究所   大学等非常勤研究員

    2003年10月 - 2004年3月

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    国名：日本国

  • 京都大学大学院   大学等非常勤研究員

    2003年5月 - 2004年3月

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    国名：日本国

  • 神戸大学 自然科学系先端融合研究環内海域環境教育研究センター 神戸大学 自然科学系先端融合研究環内海域環境教育研究センター   本務／助教

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所属学協会

  • マリンバイオテクノロジー学会

    2012年4月 - 現在

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    役職・役割名：評議員

  • 日本プランクトン学会

    2008年4月 - 現在

  • 日本水産学会

    2008年4月 - 現在

  • 日本水産学会

  • マリンバイオテクノロジー学会

  • International conference on Harmful algae

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研究分野

  • ライフサイエンス / 水圏生産科学

論文

  • The complete mitogenome of Echinoparyphium aconiatum (Digenea: Echinostomatidae) and a comparison with other digenean species 査読

    Gacad J.L.J., Tanabe-Hosoi S., Yurlova N.I., Urabe M.

    Parasitology International   92   2023年2月

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    記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Parasitology International  

    Echinoparyphium aconiatum (Digenea: Echinostomatidae) is an intestinal parasite of anatid and snail-eating birds. In Eurasia, it is also common in lymnaeid snails, which may serve as the first and second intermediate hosts. The systematics of its genus, Echinoparyphium, have long been inadequate, with poor descriptions and extensive synonymy. To provide a basis for developing new genetic markers for studies of the identification and systematics of echinostomatids, the complete Ep. aconiatum mitogenome is described and compared with other digeneans. The circular mt molecule of this species is 14,865 bp in length, with an average A + T content of 64.33%. It contains 12 protein-coding genes and 22 transfer RNA genes. The 3′ end of nad4L overlaps the 5′ end of nad4 by 40 bp, while the atp8 gene is absent. Twenty-one transfer RNA genes transcribe products with conventional cloverleaf structures, while one transfer RNA gene has unpaired D-arms. Comparative analyses indicate that Echinoparyphium aconiatum is closely related to Echinochasmus japonicus and Echinostoma miyagawai. The phylogenetic results, using our mitochondrial data indicated Ep. aconiatum as a sister taxon of Hypoderaeum conoideum in a monophyletic clade. Our data and analyses serve as the first representative sequenced mt genome from genus Echinoparpyhium, providing additional markers to clarify the taxonomic position of Ep. aconiatum.

    DOI： 10.1016/j.parint.2022.102682

    Scopus

    その他リンク： https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85138822002&origin=inward

  • Temporal variation in community structure of zoosporic fungi in Lake Biwa, Japan 査読 国際誌

    Song, P., Yi R., Tanabe S., GotoN., Seto K., Kagami M. and Ban S.

    Aquatic Microbial Ecology   2021年

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    記述言語：英語   掲載種別：研究論文（学術雑誌）   参加形態：共同（副担当）  

MISC

  • Significant yield of ectoine by Halomonas sp. EG6 during osmotic downshock treatment

    Diamino Amino Acids, Nova Science Publishers   2008年

  • Significant yield of ectoine by Halomonas sp. EG6 during osmotic downshock treatment

    2008年

  • Proposal of Pseudochattonella verruculosa gen. nov., comb. nov (Dictyochophyceae) for a formar raphidophycean alga Chattonella verruculosa, based on 18S rDNA phylogeny and ultrastructural characteristics

    Shoko Hosoi-Tanabe, Daiske Honda, Sachiko Fukaya, Isamu Otake, Yuji Inagaki, Yoshihiko Sako

    PHYCOLOGICAL RESEARCH   55 ( 3 )   185 - 192   2007年9月

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    記述言語：英語   出版者・発行元：WILEY-BLACKWELL PUBLISHING, INC  

    Chattonella verruculosa Y. Hara et Chihara was re-examined by molecular methods and microscopic examination. The 18S rDNA phylogenetic analysis clearly indicated that C. verruculosa is a member of the Dictyochophyceae, with a specific affinity to Florenciella parvula. The morphological features in C. verruculosa - namely the proximal helix with two gyres and many scattered DNA-containing areas in the chloroplasts - display the evolutionary link to the Dictyochophyceae, instead of the Raphidophyceae. Similarly, unique pyrenoid morphologies are shared between C. verruculosa and the dictyochophycean algae. Combining the molecular data and morphological characteristics, C. verruculosa is transferred to Pseudochattonella gen. nov. of the class Dictyochophyceae as Pseudochattonella verruculosa (Y. Hara et Chihara) Hosoi-Tanabe, Honda, Fukaya, Inagaki et Sako comb. nov.

    DOI： 10.1111/j.1440-1835.2007.00461.x

    Web of Science

  • Application of real-time PCR assay for detection and quantification of Alexandrium tamarense and Alexandrium catenella cysts from marine sediments

    Ryoma Kamikawa, Satoshi Nagai, Shoko Hosoi-Tanabe, Shigeru Itakura, Mineo Yamaguchi, Yoshitaka Uchida, Toshinori Baba, Yoshihiko Sako

    HARMFUL ALGAE   6 ( 3 )   413 - 420   2007年4月

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    記述言語：英語   出版者・発行元：ELSEVIER SCIENCE BV  

    The dinoflagellates Alexandrium tamarense (Lebor) Balech and Alexandrium catenella (Whedon and Kofoid) Balech (Dinophyceae) are believed to be the main species responsible for paralytic shellfish poisoning (PSP) all over the world. It is necessary to identify A. tamarense and A. catenella cysts and to monitor their distribution in sediment in order to minimize the damages caused by PSP to the economy and food quality because cysts are the seed population for blooms caused by motile vegetative cells. In this study, we developed an efficient DNA extraction method from the natural cysts present in marine sediments after they were size fractionated with a plankton net (mesh size of 20-150 mu m). The 10-3000 cysts were added to the sediments collected from the Ariake Sea, and for which the primuline-staining method did not reveal any cysts. DNA was then extracted from each sample, and linear standard curves for A. tamarense and A. catenella cysts were obtained from the correlation between the Ct values by real-time PCR and the log of the initial densities of cysts. We monitored the A. tamarense and A. catenella cyst densities in the environmental samples. This assay was demonstrated to be a powerful tool for the identification, detection, and quantification of the cysts of the toxic dinoflagellates. (C) 2007 Elsevier B.V. All rights reserved.

    DOI： 10.1016/j.hal.2006.12.004

    Web of Science

  • aurine transporter from the giant Pacific oyster: function and expression in response to hyper- and hypo-osmic stress

    Fisheries Science   73:385-394.   2007年

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  • Degradation of seaweed Wakame (Undaria pinnatifida) by composting process with inoculation of Bacillus sp. HR6. Biocontrol Science

    12:47-54.   2007年

  • Degradation of the seaweed wakame (Undaria pinnatifida) by a composting process with the inoculation of Bacillus sp. HR6

    Jing-Chun Tang, Jian-He Wei, Kenji Maeda, Hiroshi Kawai, Qixing Zhou, Shoko Hosoi-Tanabe, Shinichi Nagata

    Biocontrol Science   12 ( 2 )   47 - 54   2007年

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    記述言語：英語   出版者・発行元：Society for Antibacterial and Antifungal Agents Japan  

    Disposal of the seaweed wakame (Undaria pinnatifida) by inoculating the halotolerant bacterium Bacillus sp. HR6 was examined in an experimental scale composting system. Strain HR6 was effective in initiating the composting process of wakame, and there was a rapid increase in temperature to over 54.9-55.7°C after 18-20 h. The composting process of wakame could be carried out despite a high NaCl content, 28.2 mg/g, although lower salinity resulted in a shorter lag time and higher weight reduction. In a larger scale composting process with aeration, two peaks of temperature change were found which corresponded well to oxygen consumption and CO2 emission during the process. The pH increased to 8.83 and organic materials were reduced to 93.4 % after 72 h. The initial N and C contents were 3.9 and 34.0 %, respectively, both of which decreased during the composting process. The changes in the viable cell numbers suggested that strain HR6 predominated before 24 h and other microorganisms including HR6 were present in a mixed state during the later period of composting. The total content of alginate (TA), 32.2 % in the initial stage, decreased to 29.2 % after 72 h, while water soluble alginate (WSA) increased, indicating that the solubilization and decomposition of alginate had occurred during the composting process.

    DOI： 10.4265/bio.12.47

    Scopus

    PubMed

  • aurine transporter from the giant Pacific oyster: function and expression in response to hyper- and hypo-osmic stress

    73:385-394.   2007年

  • 貝毒研究の最前線

    恒星社   2007年

  • Development and application of fluorescence in situ hybridization (FISH) method for simple and rapid identification of the toxic dinoflagellates Alexandrium tamarense and Alexandrium catenella in culture and natural seawater

    Fisheries Science   72:77-82   2006年

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  • Phylogenetic analysis of noxious red tide flagellates Chattonella antiqua, C. marina, C. ovata, and C. verruculosa (Raphidophyceae) based on the rRNA gene family

    Fisheries Science   72:1200-1208   2006年

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  • Phylogenetic analysis of noxious red tide flagellates Chattonella antiqua, C. marina, C. ovata, and C. verruculosa (Raphidophyceae) based on the rRNA gene family

    72:1200-1208   2006年

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  • Genetic differentiation in the marine dinoflagellates Alexandrium tamarenseand Alexandrium catenellabased on DNA-DNA hybridization

    Shoko Hosoi Tanabe, Yoshihiko Sako

    Plankton and Benthos Research   1 ( 3 )   138 - 146   2006年

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    記述言語：英語  

    The genetic relationship between the toxic marine dinoflagellates Alexandrium tamarenseand Alexandrium catenella, which share similar morphological characteristics and are clustered as a sister group on the phylogenetic tree based on 28S rDNA analysis was examined by DNA-DNA hybridization. This method has contributed to the information on relative comparisons of total sequences in the field of microbiology
    however limited DNA-DNA hybridization studies have been carried out on microalgae, including dinoflagellates. The intragroup homology of DNA-DNA hybridization within the A. tamarense and A. catenellagroups, that was identified on the basis o morphotypes, was 85.0%-99.8% in both cases, whereas the intergroup homology between these two groups was 62.5%-70.3%,which was lower than the intragroup homology. These percentages revealed that not only the rDNA sequences but also the total sequence reflects slight morphological differences between A. tamarenseand A. catenella, indicating the possibility that strains identified as these two species could be differentiated as distinct groups based on the percentage obtained using DNA-DNA hybridization. The homology of A. tamarensewith other Alexandrium species was low-37.1%-45.2% with A. tamiyavanichii, 41.0%-45.5% with A. affine, and 24.8%-35.8% with A. ostenfeldii. The relatedness between A. catenellaand other Alexandriumstrains tested (e.g. between A. catenellaand A. tamiyavanichiiwas 39.0%-51.2%) was also lower than that between A. tamarenseand A. catenella. Further, these percentages were consistent with the rDNA phylogenetic analysis, indicating that DNA-DNA hybridization might be a useful tool to understand the relatedness among Alexandriumspecies. © 2006, The Plankton Society of Japan, The Japanese Association of Benthology. All rights reserved.

    DOI： 10.3800/pbr.1.138

    Scopus

  • 生物学的手法を用いたアオコ除去の可能性―琵琶湖の浄化を目指して―

    月刊水   2006年

  • 琵琶湖北湖堆積物からのUroglena americanaシストの収集とreal-time PCRによる定量法の検討

    伴 修平, 田上 琢自, 田辺(細井) 祥子

    日本陸水学会 講演要旨集   71   187 - 187   2006年

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    記述言語：日本語   出版者・発行元：日本陸水学会  

    DOI： 10.14903/jslim.71.0.187.0

  • 有害・有毒微細藻の分子モニタリング法の開発

    BRAIN TECHNO NEWS   2006年

  • Rapid detection of natural cells of Alexandrium tamarense and A-catenella (Dinophyceae) by fluorescence in situ hybridization

    S Hosoi-Tanabe, Y Sako

    HARMFUL ALGAE   4 ( 2 )   319 - 328   2005年2月

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    記述言語：英語   出版者・発行元：ELSEVIER SCIENCE BV  

    The marine toxic dinollagellates Alexandrium tamarense (Lebor) Balech and A. catenella (Whedon and Kofoid) Taylor that cause paralytic shellfish poisoning (PSP) are identified on the basis of morphological features in routine monitoring. Rapid and simple identification is, however, often difficult because of the morphological similarity. Fluorescent in situ hybridization (FISH) using ribosomal RNA (rRNA)-targeted probes has been studied as a method of easily identifying and enumerating species responsible for harmful algal blooms (HABs). Its application to monitoring natural populations of HAB species, however, is limited. Here, we applied the FISH method to identify and enumerate cells of A. tamarense and A. catenella in natural plankton assemblages collected from Japanese coastal waters. A. tamarense-specific (Atm1) and A. catenella-specific (Act1) probes were established based on the D2 region of the large-subunit ribosomal RNA gene (28S rDNA). With these two probes, natural cells of A. tamarense or A. catenella in field samples could easily be identified when the following three conditions were met. First, cells should be concentrated by filtration, not centrifugation, in order to avoid the loss of cells. Second, autofluorescence should be minimized; acetone was an effective decolorization reagent. Third, samples should be stored at -20 or -80 degreesC for long-term preservation. The results indicate that FISH is a useful tool for the rapid identification of toxic Alexandrium. spp. and can facilitate the analysis of numerous natural samples. (C) 2004 Elsevier B.V. All rights reserved.

    DOI： 10.1016/j.hal.2004.04.002

    Web of Science

  • Development of a quantification assay for the cysts of the toxic dinoflagellate Alexandrium tamarense using real-time polymerase chain reaction

    Fish. Sci.   71: 985-989   2005年

  • Molecular detection of Uroglena americana (Chrysophyceae),a freshwater red-tide agent in Lake Biwa, Japan

    29: 1103-1106   2005年

  • Identification of a gene induced in conjugation-promoted cells of toxic marine dinoflagellate Alexandrium tamarense and Alexandrium catenella using differential display analysis.

    FEMS Microbiology Letter   25: 1161-168   2005年

  • Development of a quantification assay for the cysts of the toxic dinoflagellate Alexandrium tamarense using real-time polymerase chain reaction.

    Fisheries Science   71: 985-989   2005年

  • Species specific detection and quantification of the toxic marine dinoflagellates Alexandrium tamarense and A. catenella by real-time polymerase chain reaction.

    Mar. Biotecnol.   7: 506-514.   2005年

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  • Molecular detection of Uroglena americana (Chrysophyceae),a freshwater red-tide agent in Lake Biwa, Japan

    Verh. Internat. Verein. Limnol.   29: 1103-1106   2005年

  • Identification of a gene induced in conjugation-promoted cells of toxic marine dinoflagellate Alexandrium tamarense and Alexandrium catenella using differential display analysis.

    FEMS Microbiology Letter   25: 1161-168   2005年

  • Sequence and polymerase chain reaction?restriction fragment length polymorphism analysis of the large subunit rRNA gene of bivalve: Simple and widely applicable technique for multiple species identification of bivalve larva.

    Fish. Sci.   70: 629?637.   2004年

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  • Species-specific detection and quantification of the toxic dinoflagellate Alexandrium tamarense by Taq Man PCR usning 5’-3’ exonuclease activity.

    Mar. Biotecnol.   6: S30-S24.   2004年

  • Development of fluorescence in situ hybridization (FISH) method using rRNA-targeted probes for simple and rapid detection of the toxic dinoflagellates Alexandrium tamarense and A. catenella.

    J. Phycol.   40:598-605.   2004年

  • Development of fluorescence in situ hybridization (FISH) method using rRNA-targeted probes for simple and rapid detection of the toxic dinoflagellates Alexandrium tamarense and A. catenella

    Journal of Phycology   40:598-605.   2004年

  • DNA-DNA hybridization method as one of taxonomic criteria on marine dinoflagellate genus <i>Alexandrium.</i>

    International Journal of Systematic and Evolutionary Microbiology   2002年

  • Simple and rapid detection of the toxic marine dinoflagellates Alexandrium tamarense and A. catenella with fluorescence in situ hybridization (FISH) using rRNA-targeted probes.

    Fish. Sci.   68: 515-518.   2002年

  • Simple and rapid detection of the toxic marine dinoflagellates Alexandrium tamarense and A. catenella with fluorescence in situ hybridization (FISH) using rRNA-targeted probes.

    68: 515-518.   2002年

  • Simple and rapid detection of the toxic marine dinoflagellates Alexandrium tamarense and A. catenella with fluorescence in situ hybridization (FISH) using rRNA-targeted probes

    Fisheries Science   68巻 515-518頁   2002年

  • Simple and rapid detection of the toxic marine dinoflagellates Alexandrium tamarense and A. catenella with fluorescence in situ hybridization (FISH) using rRNA-targeted probes

    Fisheries Science   2002年

  • Fluorescent <i>in situ</i> hybridization (FISH) method with rRNA-targeted probes for sensitive detection of toxic marine dinoflagellates <i>Alexndrium tamarense</i> and <i>A. catenella</i> in natural sea water.

    Journal of Phycology   2002年

  • Quantitative detection of the toxic marine dinoflagellates <i>Alexandrium tamarense</i> and <i>A. catenella</i> within one hour with fluorescence in situ hybridization using fRNA-tageted probes.

    Journal of Phycology   2002年

  • Sensitive detection of the marine toxic dinoflagellates Alexandrium tamarense and A.catenella from natural Japanese coastal water with rRNA-targeted probes

    Internationnal Commemorative Symposium 70th Anniversary of the Japanese Society of Fisheries Science   68 ( supl )   515 - 518   2001年9月

  • Fluorescence in situ hybridization with rRNA targeted probes for Alexandrium tamarense and A. catenella in natural population

    Ninth International Conference on Harmful Algae   2000年2月

  • 微細藻類の分子分類(共著)

    月刊海洋   21   78 - 79   2000年

  • Fluorescence in situ hybridization with rRNA targeted probes for Alexandrium tamarense and A. catenella in natural sea water.

    International conference Harmful algae   2000年

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受賞

  • 第54回日本生態学会ポスター優秀賞

    2007年  

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    受賞国：日本国

  • 平成16年度日本水産学会論文賞

    2005年  

共同研究・競争的資金等の研究課題

  • 有毒渦鞭毛藻Alexandrium属の遺伝子診断法の開発

    2002年 - 2004年

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    資金種別：競争的資金

  • Development of method for monitoring of the genus Alexandvium using molecular biology

    2000年 - 2003年

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    資金種別：競争的資金

  • 有毒渦鞭毛藻Alexandrium属の環境変化に対する生存戦略機構の解明

    2000年 - 2003年

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    資金種別：競争的資金

  • 有毒渦鞭毛藻Alexandrium属の休眠細胞(シスト)形成に関する遺伝子の探究

    2000年 - 2003年

    　 詳細を見る

    資金種別：競争的資金

  • Envrionmental replying of Alexandrium Spp.

    2000年 - 2003年

    　 詳細を見る

    資金種別：競争的資金

  • Individual group analysis using mitochondrial DNA for Alexandrium Spp

    　 詳細を見る

    資金種別：競争的資金

  • Gene about the cyst forming of Alexandrium

    　 詳細を見る

    資金種別：競争的資金

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講演・口頭発表等

  • 琵琶湖におけるヨコエビの動態

    市原龍，細井祥子

    2021年ベントス・プランクトン学会合同大会  2021年9月 

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    記述言語：日本語   会議種別：ポスター発表  

  • Distribution of toxic Alexandrium tamaense and A.catenella in Akkeshi-ko estuary and Akkeshi Bay, where oyster is cultivated on a large scale in Japan 国際会議

    Tanabe, S., Suzukawa, S. and D. Kashima

    Forth Asia Marine Biology Symposium (AMBS 2019)  2019年11月 

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    記述言語：英語   会議種別：ポスター発表  

    開催地：Taipei, Taiwan  

  • いらないものを利用する−海洋性廃棄物を利用した環境保全− 招待

    細井祥子

    大学院研究交流会2019  2019年9月 

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    記述言語：日本語   会議種別：口頭発表（一般）