記述言語：英語   掲載種別：研究論文（学術雑誌）   参加形態：共同（主担当）  

Thermal isomerization of (all-E)-lycopene to the corresponding Z-isomers was investigated in edible vegetable oils; perilla, linseed, grape seed, soybean, corn, sesame, rapeseed, rice bran, safflower seed, olive, and sunflower seed oil. Purified (all-E)-lycopene from tomato oleoresin was converted to Z-isomers in the range of 44.8 to 58.8% content, and the remaining ratio of total amount of lycopene isomers without decomposition were ranged from 38.8 to 79.6% after heating at 100 °C for 1 hour in the vegetable oils. Both values were exceedingly high in sesame oil; 58.8% of total Z-isomers content and 78.3% of remaining lycopene. In particular, (5Z)-lycopene, which has higher bioavailability and antioxidant capacity as well as greater storage stability among the Z-isomers, was notably increased in that oil; approximately threefold higher than the average of the other vegetable oils.

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Elsevier   参加形態：共同（副担当）  

To identify specific inhibitors of the human secretary pathway Ca2+-ATPase 2 (hSPCA2), a recombinant hSPCA2 was expressed in Saccharomyces cerevisiae, and purified by Co2+-chelating chromatography. The isolated hSPCA2 catalyzed ATP hydrolysis in the presence of Ca2+ ions. The Ca2+ dissociation constant for ATPase activation was 25 nM. hSPCA2 activity was inhibited by thapsigargin, 2,20-methylenebis(6-tertbutyl-p-cresol), and 4-octylphenol in the low-micromolar concentration range. Unexpectedly, the organic solvent wash from standard laboratory polypropylene microtubes showed strong inhibitory potency toward hSPCA2 activity. The extract was found to comprise mainly primary fatty acid amides (PFAAs) by NMR analysis. Individual PFAAs, especially oleamide and linoleamide, almost completely inhibited hSPCA2 activity with IC50 values of 7.5 μM and 3.8 μM, respectively.

DOI： 10.1016/j.bbrc.2016.06.055

記述言語：英語   掲載種別：研究論文（学術雑誌）   参加形態：共同（副担当）  

In the title compound, C14 H6 N2 O8 , the anthraquinone unit is essentially planar [maximum deviation = 0.0645 (10) Å ], and there are two intramolecular O—H⋯ O hydrogen bonds forming S (6) motifs. The planes of the two nitro substituents make dihedral angles of 54.77 (8) and 55.60 (3)° with the anthraquinone ring system. In the crystal, molecules are linked by short intermolecular O⋯ O contacts, leading to a three-dimensional network structure

記述言語：英語   掲載種別：研究論文（学術雑誌）   参加形態：共同（副担当）  

In the mol­ecule of the title compound, C17H13BrO4, the anthra­quinone ring system is slightly bent, with a dihedral angle of 169.99 (7)° between the planes of the two benzene rings. The side chain (O—C—C—C—Br) has a gauche–gauche conformation, as indicated by the O—C—C—C and C—C—C—Br torsion angles of −66.9 (2) and −65.8 (2)°, respectively. In addition, there is an intra­molecular O—H⋯O hydrogen bond enclosing an S(6) ring motif. The hydrogen-bond donor is bifurcated; in the crystal, a pair of O—H⋯O hydrogen bonds connects two mol­ecules, forming an inversion dimer with an R22(12) ring motif.

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Elsevier   参加形態：共同（主担当）  

(15Z)-Lycopene was prepared by thermal isomerization of (all-E)-lycopene derived from tomatoes, and isolated by using a series of chromatographies. The fine red crystalline powder of (15Z)-lycopene was obtained from 556 mg of (all-E)-lycopene with a yield of 0.6 mg (purity: reversed-phase HPLC, 97.2%; normal-phase HPLC, ≥99.9%), and 1H and 13C NMR spectra of the isomer were fully assigned. More refined computational analyses that considered differences in the energy levels of the conformers involved in isomerization have also determined the stabilities of (15Z)-lycopene and other geometric isomers, along with the activation energies during isomerization from the all-E form. The fine control of conditions for HPLC separation and an advanced theoretical insight into geometric isomerization have led to the discovery of the 15Z-isomer generated from a natural source.

DOI： 10.1016/j.bbrc.2015.09.122

記述言語：英語   掲載種別：研究論文（学術雑誌）   参加形態：共同（副担当）  

Catalytic isomerization of (all-E)-lycopene to Z-isomers using iron(III) chloride was investigated and optimized under various conditions of solvents, concentrations of iron(III) chloride, and reaction temperatures. The total contents of Z-isomers converted were higher in the order of CH2Cl2 (78.4%) > benzene (61.4%) > acetone (51.5%) > ethyl acetate (50.8%) at 20 °C for 3 h using 1.0 × 10−3 mg/mL iron(III) chloride for 0.1 mg/mL (all-E)-lycopene. However, the decomposition of lycopene was markedly accelerated in CH2Cl2: the remaining lycopene after the reaction for 3 h and 12 h was only 79.4% and 47.5%, respectively. As the concentration of catalyst increased in acetone, the Z-isomerization ratio of lycopene increased to more than 80%, followed by rapid degradation of lycopene to undetectable levels using > 4.0 × 10−3 mg/mL iron(III) chloride with the above concentration of (all-E)-lycopene. Finally, greater isomerization (79.9%) was attained at 60 °C in acetone for 3 h in the presence of 1.0 × 10−3 mg/mL iron(III) chloride, largely without decomposition of lycopene (remaining ratio of total amount of lycopene isomers after the reaction, 96.5%).

記述言語：英語   掲載種別：研究論文（学術雑誌）   参加形態：共同（副担当）  

記述言語：英語   掲載種別：研究論文（学術雑誌）   参加形態：共同（副担当）  

Recrystallization of 1,4-dipropoxy-9,10-anthraquinone from hexane solution afforded two polymorphs: red prisms and yellow needles. X-ray crystallographic analyses revealed that the molecular arrangement was an anti-parallel orientation for red solids and a slipped-parallel orientation for yellow solids. The difference in solid-state color was analyzed by TD-DFT calculations of their multimers.

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Elsevier   参加形態：共同（副担当）  

The geometric isomerisation of (all-E)-lycopene, purified from tomato paste, was investigated in various organic solvents. Isomerisation ratios to the Z-isomers of lycopene in CH2Cl2 and CHCl3 over 24 h were calculated to be 19.7% and 11.4% at 4 ºC and 77.8% and 48.4% at 50 ºC, respectively. In CH2Br2, more than 60% was attained in the first several hours, independent of temperature. The predominant Z-isomers obtained thermally, (9Z)-lycopene and (13Z)-lycopene, were purified and their absorption maxima and molar extinction coefficients in hexane were determined for the first time. Absorption values at 460 nm were also measured for both Z-isomers along with (all-E)-lycopene to accurately evaluate their concentrations by HPLC analysis. This approach successfully revealed that (13Z)-lycopene formed predominantly in benzene or CHCl3 at 50 ºC; in contrast, the 5Z-isomer was preferentially obtained in CH2Cl2 or CH2Br2.

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ACS Publications   参加形態：共同（副担当）  

Photoisomerization of (all-E)-lycopene to the corresponding Z-isomers was investigated under visible to middle-infrared light irradiation in the presence of several sensitizers, including edible ones. Highly purified (all-E)-lycopene from tomato paste was isomerized to Z-isomers to the extent of 46.4–57.4% after irradiation with the sensitizers for 60 min in acetone, in which a thermodynamically-stable isomer of (5Z)-lycopene was predominantly generated, while kinetically-preferable (9Z)- and (13Z)-lycopene were dominant without sensitizer. Examination of the time course of photoisomerization demonstrated that the highest isomerization efficiency (80.4%) was attained using erythrosine as the sensitizer under 480–600 nm light irradiation in hexane for 60 min, a protocol which successfully suppressed the degradation of lycopene. (5Z)-Lycopene, reported as a more bioavailable isomer, was again predominantly produced with erythrosine and rose bengal in each solvent.

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Wiley   参加形態：共同（副担当）  

In an attempt to brominate 1,4-dipropoxy-9,10-anthra-quinone, a mixture of products, including the title compound, C14H7BrO4, was obtained. The molecule is essentially planar (r.m.s. deviation = 0.029 A˚ ) and two intramolecular O—H···O hydrogen bonds occur. In the crystal, the molecules are linked by weak C—H·O hydrogen bonds, Br··O contacts [3.240 (5) A˚ ], and π–π stacking interactions [shortest centroid–centroid separation = 3.562 (4) A˚ ], generating a three-dimensional network.

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：ACS Publications   参加形態：共同（主担当）  

A large amount of (all-E)-lycopene was successfully purified from tomato paste using an improved method that included a procedure to wash crystalline powder with acetone. The total yield of the pure (all-E) form was at least 30%. The melting point of (all-E)-lycopene was determined to be 176.35 ºC by DSC measurements. Bathochromic shifts were observed in the absorption maxima of all solvents tested (at most a 36 nm shift for λ2 in carbon disulfide, as was observed in hexane), and were accompanied by absorbance decreases, namely a hypochromic effect, showing a higher correlation between the position and the intensity of the main absorption bands. This bathochromic shift was dependent on the polarizability of the solvent rather than its polarity. The rate constants of the decrease in (all-E)-lycopene with hexane and benzene were calculated to be 3.19 × 10−5 sec−1 and 3.55 × 10−5 sec−1, respectively.

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：The Japan Society for Bioscience, Biotechnology, and Agrochemistry   参加形態：共同（主担当）  

A novel esterase showing activity specific for esters of aryl-carboxylic acids was discovered in Sporosarcina sp. nov., which was identified by the 16S rDNA sequencing method in addition to morphological and physiological analyses. The aryl-carboxylesterase (named EstAC) was purified 780-fold from crude cell extracts by a 5-step procedure. EstAC was characterized as a monomeric protein with a molecular weight of 43,000, an optimum pH of around 9.0, and an optimum temperature of 40°C. The pH optimum and the effects of inhibitors together with an internal amino acid sequence suggested that EstAC is a member of family VIII esterases. EstAC was found to be highly active on a wide variety of substrates such as alkyl benzoates, alkyl phenylacetates, ethyl α- or β-substituted phenylpropionates, dialkyl terephthalates, dimethyl isophthalate, and ethylene glycol dibenzoate. However, monomethyl terephthalate was not hydrolyzed. It was suggested that EstAC had 4-hydroxybenzoyl and cinnamoyl esterase activities as well.

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Springer   参加形態：共同（主担当）  

Poly(e-L-lysine) (e-PL) is a naturallyoccurring
L-lysine homopolymer having antimicrobial
activity. A newly-isolated strain of Streptomyces
aureofaciens produced a short chain length e-PL
consisting of 5–20 residues at the highest production
level of 4.5 g l–1. This e-PL had different spectra in
terms of antimicrobial activity from the e-PL that is
now used as a food preservative. The high productivity
was based on multiple metabolic pathways for
L-lysine synthesis, and a great flux from L-lysine to
e-PL. The usefulness of this new e-PL and its
producing strain was discussed.

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Wiley-Blackwell   参加形態：共同（主担当）  

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Springer   参加形態：共同（副担当）  

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Springer   参加形態：共同（副担当）  

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Springer   参加形態：共同（副担当）  

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Japan Society for Bioscience, Biotechnology, and Agrochemistry   参加形態：共同（主担当）  

記述言語：日本語   会議種別：口頭発表（一般）  

記述言語：日本語   会議種別：口頭発表（一般）  

記述言語：日本語   会議種別：口頭発表（一般）  

記述言語：日本語   会議種別：口頭発表（一般）  

記述言語：日本語   会議種別：口頭発表（一般）  

記述言語：日本語   会議種別：口頭発表（一般）  

記述言語：日本語   会議種別：口頭発表（一般）  

記述言語：日本語   会議種別：口頭発表（一般）  

記述言語：日本語   会議種別：口頭発表（一般）  

記述言語：日本語   会議種別：口頭発表（一般）  

記述言語：日本語   会議種別：口頭発表（一般）  

記述言語：日本語   会議種別：口頭発表（一般）  

記述言語：英語   会議種別：ポスター発表  

記述言語：日本語   会議種別：ポスター発表  

記述言語：日本語   会議種別：口頭発表（一般）  

記述言語：日本語   会議種別：口頭発表（一般）  

記述言語：日本語   会議種別：口頭発表（一般）  

記述言語：日本語   会議種別：口頭発表（一般）  

記述言語：日本語   会議種別：ポスター発表  

記述言語：日本語   会議種別：口頭発表（一般）  

記述言語：英語   会議種別：ポスター発表  

記述言語：日本語   会議種別：口頭発表（一般）  

記述言語：日本語   会議種別：ポスター発表  

記述言語：日本語   会議種別：ポスター発表  

記述言語：日本語   会議種別：口頭発表（一般）  

開催地：熊本大学  

記述言語：日本語   会議種別：ポスター発表  

記述言語：日本語   会議種別：ポスター発表  

記述言語：日本語   会議種別：ポスター発表  

記述言語：日本語   会議種別：ポスター発表  

記述言語：日本語   会議種別：口頭発表（一般）  

記述言語：日本語   会議種別：口頭発表（一般）  

記述言語：日本語   会議種別：口頭発表（一般）  

開催地：アスティとくしま  

記述言語：英語   会議種別：ポスター発表  

記述言語：英語   会議種別：ポスター発表  

記述言語：日本語   会議種別：口頭発表（一般）  

記述言語：日本語   会議種別：口頭発表（一般）  

記述言語：日本語   会議種別：ポスター発表  

芳香族カルボン酸エステルに対し加水分解活性を示す新規エステラーゼをSporosarcina属細菌eSP04株より見出し1)，当該酵素をコードする遺伝子をクローニング，塩基配列を決定後，大腸菌で発現させた。精製した本酵素EstACは分子量43,000の単量体で，安息香酸エチルを基質として用い，反応の至適pH 9.0，至適温度40 °Cであることが示された。また本酵素は種々の芳香族カルボン酸エステルに加え，フェニル酢酸エステル，桂皮酸エステルや短鎖脂肪酸エステルにも加水分解能を示した。EstACの一次構造および二次構造解析を行い，関連酵素群との系統樹を作成した。さらに，高いアミノ酸配列相同性を示す加水分解酵素を鋳型として用いたホモロジーモデリングにより，EstACの三次元構造を構築した。推定活性中心に存在するアミノ酸残基セリン69，リシン72，およびチロシン180が芳香族基質の加水分解に密接に関与していることが示唆された。

記述言語：日本語   会議種別：ポスター発表  

記述言語：日本語   会議種別：口頭発表（一般）  

開催地：金沢大学