Faculty Profiles - TAKAKURA Koichi

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Reproductive habitat use, body-size structure and spawning season of Cobitis minamorii minamorii in paddy fields and ditches Reviewed

Morii K., Kitano D., Kanai R., Takakura K.I.

Ichthyological Research 69 ( 1 ) 182 - 188 2022.1

Language：English Publishing type：Research paper (scientific journal) Publisher：Ichthyological Research

We surveyed the reproductive ecology of Cobitis minamorii minamorii in paddy fields and ditches. The spatial distribution of females in the spawning season differed from that in the non-spawning season, even in short ditches. Eggs were collected in both ditches and paddy fields, unlike an earlier study demonstrating that no eggs were collected in ditches. The frequency distributions of standard body lengths of males and females were unimodal and bimodal, respectively. The females with a large standard body length were expected to be gravid with higher probability in May.

DOI： 10.1007/s10228-021-00826-1

Oviposition preference of solanum fruit fly Bactrocera latifrons (Diptera: Tephritidae) invaded Ryukyu Islands, Southwest Japan Reviewed

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Comparisons among populations and individuals to evaluate pollen-pistil interaction as a mechanism of reproductive interference in Taraxacum.

Keisuke Hashimoto, Akane Yamamoto, Masahiro M Kanaoka, Akiyo Naiki, Ko-Ichi Takakura, Sachiko Nishida

Journal of plant research 135 ( 1 ) 29 - 40 2022.1

Language：Japanese Publishing type：Research paper (scientific journal)

Reproductive interference (RI), an interspecific mating interaction that reduces the fitness of at least one of the species involved, can lead to exclusive distributions in closely related species. A hypothesis previously proposed is that RI in plants may occur by ovule usurpation, in which pistils lack interspecific incompatibility and mistakenly accept heterospecific pollen, thereby losing an opportunity for conspecific pollen fertilization. However, few comparative studies have evaluated the consistency of the inferred mechanism within and among individuals and populations. We conducted hand-pollination experiments in six populations of three native Taraxacum species that suffered from different levels of RI from an alien congener, T. officinale, and compared pollen-pistil interactions among populations. We also investigated the interactions for eight individual T. japonicum plants whose response to heterospecific pollen deposition had been previously measured. Our results revealed that pollen tubes often penetrated native ovaries following heterospecific pollination in populations suffering from strong RI, whereas they seldom did in populations suffering from marginal RI. However, the relative frequency of the pollen tube penetration was not significantly related to the strength of alien RI. Not all pistils on an individual plant showed the same pollen receptivity following heterospecific pollination; rather, some accepted and some refused the pollen tubes. The relationship between pollen tube penetration following heterospecific pollination and the strength of the alien RI was also not significant among individuals. Our present results generally support the ovule usurpation hypothesis, but suggest that other factors, such as competition for pollinator services, variation in the effects of heterospecific pollen donors, and condition of the native inflorescences, might also affect the observed RI strength.

DOI： 10.1007/s10265-021-01352-x

PubMed

Hisaoka, K., Honma, A, Matsuyama, T., Matsuura, Y., Nishida, T. and Takakura, KI

Japanese Journal of Environmental Entomology and Zoology 34 1 - 8 2022

Differential performance of contrasting defensive traits of cocoons of two moth species against bird predation Reviewed

Furukawa M., Nakanishi K., Honma A., Takakura K.I., Matsuyama K., Hidaka N., Sawada H., Nishida T.

Entomological Science 24 ( 3 ) 261 - 269 2021.9

Language：English Publishing type：Research paper (scientific journal) Publisher：Entomological Science

Prey defensive traits against predators can be categorized into primary defense (avoiding detection) and secondary defense (avoiding attack after detection). There are trade-offs between these two defensive traits, which consider the cost of defense. To quantify the predation avoidance efficacy of both defensive traits against common predators, we compared bird predation pressures and the corresponding avoidance measures of cocoons of two nettle moth species, which utilize contrasting avoidance strategies: Parasa lepida (investing predominantly in primary defense) and Monema flavescens (investing predominantly in secondary defense). Field censuses revealed that bird predation was the most significant mortality factor for both species. The survival rate of cocoons was significantly higher for M. flavescens than for P. lepida, although M. flavescens were more conspicuous than P. lepida. Measurement of prepupae (cocoon content) mass, and experiments on the feeding preferences of the prepupae of both species using chicks, confirmed a similar quantity and quality of both speciesʼ prepupae as prey items. Our results were explained by the advantage of cocoon hardness outweighing the disadvantage of cocoon conspicuousness. The predation behavior of wild birds suggested that they spent considerably longer handling than searching for cocoons, which accounted for their cocoon preference for P. lepida over M. flavescens in the context of optimal foraging theory. Our results suggested that secondary defense was more effective in reducing bird predation than primary defense in limacodid cocoons.

DOI： 10.1111/ens.12482

he striped fruit fly Zeugodacus scutellatus (Diptera: Tephritidae) uses female flowers of Trichosanthes kirilowii var. japonica as host in Shiga Prefecture, Central Japan Reviewed

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Differential performance of contrasting defensive traits of cocoons of two moth species against bird predation

24 ( 3 ) 261 - 269 2021.9

Reproductive habitat use, body-size structure and spawning season of Cobitis minamorii minamorii in paddy fields and ditches Reviewed

2021.6

Kitano, D. and K. I. Takakura

Japanese Journal of Environmental Entomology and Zoology 32 111 - 115 2021

Simple and on-site DNA purification for LAMP reaction applicable to non-adult tephritid fruit fly (Diptera: Tephiritidae) Reviewed

Kitano D., Takakura K.I.

Journal of Applied Entomology 144 ( 9 ) 824 - 829 2020.11

Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Applied Entomology

Tephritid fruit flies are important pests of fruits and vegetables worldwide, but few studies have examined their ecology and life history in their native range because non-adult individuals of closely related species are morphologically indistinguishable. To identify non-adult individuals collected from host plants, flies examined in earlier studies were painstakingly reared to adulthood. Using Zeugodacus scutellatus Hendel as a model species, this study established a simple DNA extraction and purification method based on loop-mediated isothermal amplification (LAMP) reaction for on-site molecular identification of non-adult fruit flies. The non-adult individual body fluids were sampled using a sterilized toothpick and then diluted in TE buffer and heated for a short time. Alternatively, eggs were crushed in TE buffer using a sterilized toothpick and then heated. After heated buffer was added to the LAMP reaction mixture as template DNA and the mixture was heated for incubation of polymerase, the LAMP reaction detected the amplified DNA of Z. scutellatus. This purification method, characterized by heating after dilution with TE buffer, is simple and rapid. Our purification method, which obviates large centrifuges, thermal cyclers, and other apparatus, provides lower-cost and better on-site molecular identification of non-adult fruit flies than methods described in earlier reports. The LAMP-based molecular identification technique and our purification method are particularly useful for studying quarantined pest organisms that cannot be removed from their native areas.

DOI： 10.1111/jen.12820

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Simple and on-site DNA purification for LAMP reaction applicable to non-adult tephritid fruit fly (Diptera: Tephiritidae)

Daisuque Kitano, Koh-Ichi Takakura

Journal of Applied Entomology 144 ( 9 ) 824 - 829 2020.11

Language：English Publishing type：Research paper (scientific journal) Publisher：Blackwell Publishing Ltd

DOI： 10.1111/jen.12820

Habitat partitioning of two closely related pond frogs, Pelophylax nigromaculatus and Pelophylax porosus brevipodus, during their breeding season Reviewed

Nakanishi K., Honma A., Furukawa M., Takakura K.I., Fujii N., Morii K., Terasawa Y., Nishida T.

Evolutionary Ecology 34 ( 5 ) 855 - 866 2020.10

Language：English Publishing type：Research paper (scientific journal) Publisher：Evolutionary Ecology

Parapatric distributions of closely related species are common in many taxonomic groups. However, habitat overlap in sympatric areas within both species’ ranges is complex and driven by the nature of the interactions between species as well as each species’ ecological requirements. We therefore investigated the distribution of breeding habitats for two congeneric frogs, Pelophylax nigromaculatus and Pelophylax porosus brevipodus, in a region of Japan where these species co-occur, based on observation of mating calls as an index of the abundance of males engaging in reproductive activities. Among a total of 381 surveyed sites, P. nigromaculatus and P. porosus brevipodus were found at 171 and 229 sites, respectively. Pelophylax porosus brevipodus was widely distributed throughout the study area and was more abundant than P. nigromaculatus in sympatric sites. Analysis using cumulative link mixed models revealed that the abundance of calling males was negatively affected by the presence of calling males of the other species and by landscape factors. Interspecific differences in this negative effect appear to favor P. porosus brevipodus over P. nigromaculatus in our study area. Due to differences in the primary habitat preferences of the two species during the non-breeding season, habitat partitioning during the breeding season is likely driven by negative interspecific interactions during the reproductive process (i.e., reproductive interference).

DOI： 10.1007/s10682-020-10061-1

Quantitative estimation of reproductive interference between two alien pillbugs introduced into Japan. Reviewed

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Habitat partitioning of two closely related pond frogs,Pelophylax nigromaculatusandPelophylax porosus brevipodus, during their breeding season

34 ( 5 ) 855 - 866 2020.10

Koh-Ichi Takakura, Teruya Inoue

Journal of Environmental Entomology and Zoology 31 ( 2 ) 43 - 50 2020.9

Habitat partitioning in native Geranium species through reproductive interference Reviewed

Nishida S., Takakura K.I., Naiki A., Nishida T.

Annals of botany 125 ( 4 ) 651 - 661 2020.3

Language：English Publishing type：Research paper (scientific journal) Publisher：Annals of botany

© The Author(s) 2020. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com. BACKGROUND AND AIMS: Heterospecific pollen transfer may reduce the fitness of recipient species, a phenomenon known as reproductive interference. A theoretical study has predicted that distributions of species pairs affected by reproductive interference may be syntopic under negligible reproductive interference, sympatric but with partitioning at small spatial scale (i.e. allotopic) under weak interference, or exclusive when reproductive interference is strong. Verifying these predictions is essential for evaluation of the applicability of reproductive interference as a general assembly rule of biological communities. The aim of this study was to test these predictions in two sympatrically distributed wild Geranium species, G. thunbergii and G. wilfordii. METHODS: To measure the effect of reproductive interference, the associations between the relative abundance of the counterpart species and seed set in the focal species, and seed set reduction following mixed pollination, were analysed. The possibility of hybridization with viable offspring was examined by genotyping plants in the field and after mixed pollination. Fertility of putative hybrids was based on their seed set and the proportion of pollen grains with apertural protrusions. A transect study was conducted to examine spatial partitioning, and possible influences of environmental conditions (canopy openness and soil moisture content) on partitioning between the species were analysed. KEY RESULTS: Neither abundance of the counterpart species nor heterospecific pollen deposition significantly affected seed set in the focal species, and hybridization between species was almost symmetrical. Putative hybrids had low fertility. The two species were exclusively distributed at small scale, although environmental conditions were not significantly different between them. CONCLUSIONS: The allotopy of the two species may be maintained by relatively weak reproductive interference through bidirectional hybridization. Re-evaluation of hybridization may allow ongoing or past reproductive interference to be recognized and provide insight into the distributional relationships between the interacting plants.

DOI： 10.1093/aob/mcz210

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Habitat partitioning in native Geranium species through reproductive interference. Reviewed International journal

2020.1

Inhomogeneity of sediment samples in analysis of hexabromocyclododecane Reviewed

Ichihara M., Yamamoto A., Kakutani N., Takakura K., Sudo M.

Environmental Monitoring and Assessment 191 ( 7 ) 2019.7

Language：English Publishing type：Research paper (scientific journal) Publisher：Environmental Monitoring and Assessment

© 2019, Springer Nature Switzerland AG. The repeatability test of the analytical method for hexabromocyclododecane (HBCD) was conducted with sediment sample. The maximum HBCD concentration exceeded the minimum by a factor of 90 even though the identical sediment samples were used. Therefore, we examined which step of the analytical method was the factor causing variability. We examined the blank test, and confirmation test of the extraction and purified procedure. From these results, we confirmed that there was nothing wrong with the accuracy of our analytical method. These results indicate that the variability of HBCD concentration in the repeatability test was attributed not to the analytical method, but to the inhomogeneity of the sediment sample. Aluminum, silicon, and organic carbon in sediments were measured to compare the variability of these concentrations with that of HBCD concentration. These concentrations were similar values within identical samples which showed variability in HBCD concentration. HBCD concentration in several samples did not correlate with organic carbon content. These results suggests that sediment samples were homogeneous by itself, and HBCD was heterogeneously distributed in spite of homogeneity of organic carbon in sediment. The sediment sample with variability in HBCD concentration showed different HBCD diastereomer compositions in identical sediment. It implies that the sample contained HBCD derived from different histories or point sources. Even though we increased sample amounts to analyze the homogeneity of the sample, HBCD concentration varied within identical samples if the sample had a hot spot. Past monitoring data may contain overestimation or underestimation of HBCD concentration in sediment.

DOI： 10.1007/s10661-019-7553-6

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Comparison of mechanisms of reproductive interference in Taraxacum. Reviewed International journal

Akane Takemori, Akiyo Naiki, Ko-Ichi Takakura, Masahiro M Kanaoka, Sachiko Nishida

Annals of botany 123 ( 6 ) 1017 - 1027 2019.6

Language：English Publishing type：Research paper (scientific journal)

BACKGROUND AND AIMS: Reproductive interference may reduce fitness of either of the involved species, with potentially important ecological and evolutionary consequences. Except for the effect of shared pollinators on reproductive success, however, mechanisms underlying reproductive interference have been little studied, even though the severity of its impact may depend on the specific mechanism. The aim of this study was therefore to explore the mechanisms of reproductive interference between Taraxacum japonicum (native to Japan) and Taraxacum officinale (alien). METHODS: In a field survey, the association between alien species density and seed set in T. japonicum, and whether pollinator behaviour indicated a preference for the alien, were examined. Effects of heterospecific pollen deposition were measured in a series of hand pollination experiments, including mixed pollination experiments in which the order of application of conspecific and heterospecific pollen was varied. Finally, to investigate hybridization frequency, the parentage of seedlings produced following natural, mixed or heterospecific pollination was compared. KEY RESULTS: Alien species density did not negatively affect native seed set, nor did pollinators appear to have a preference for alien flowers. The hand pollination experiments showed that heterospecific pollen deposition adversely affected native seed set, especially when alien pollen was applied before conspecific pollen. No viable hybrids were found following natural pollination, which suggests that hybridization might be a rare event. CONCLUSION: Among the examined mechanisms, heterospecific pollen deposition might have the largest deleterious effect on the native species. This effect is frequency dependent; thus, a positive feedback loop may cause the effect on the population dynamics to increase over time, with the result that the alien might eventually displace the native in a population. Effects of the examined mechanisms on population dynamics should be investigated further to improve understanding of the impact of reproductive interference on the structure of plant communities.

DOI： 10.1093/aob/mcz007

PubMed

Comparison of mechanisms of reproductive interference in Taraxacum Reviewed

Takemori A., Naiki A., Takakura K.I., Kanaoka M.M., Nishida S.

Annals of Botany 123 ( 6 ) 1017 - 1027 2019.1

Language：English Publishing type：Research paper (scientific journal) Publisher：Annals of Botany

© The Author(s) 2018. Background and Aims Reproductive interference may reduce fitness of either of the involved species, with potentially important ecological and evolutionary consequences. Except for the effect of shared pollinators on reproductive success, however, mechanisms underlying reproductive interference have been little studied, even though the severity of its impact may depend on the specific mechanism. The aim of this study was therefore to explore the mechanisms of reproductive interference between Taraxacum japonicum (native to Japan) and Taraxacum officinale (alien). • Methods In a field survey, the association between alien species density and seed set in T. japonicum, and whether pollinator behaviour indicated a preference for the alien, were examined. Effects of heterospecific pollen deposition were measured in a series of hand pollination experiments, including mixed pollination experiments in which the order of application of conspecific and heterospecific pollen was varied. Finally, to investigate hybridization frequency, the parentage of seedlings produced following natural, mixed or heterospecific pollination was compared. • Key Results Alien species density did not negatively affect native seed set, nor did pollinators appear to have a preference for alien flowers. The hand pollination experiments showed that heterospecific pollen deposition adversely affected native seed set, especially when alien pollen was applied before conspecific pollen. No viable hybrids were found following natural pollination, which suggests that hybridization might be a rare event. • Conclusion Among the examined mechanisms, heterospecific pollen deposition might have the largest deleterious effect on the native species. This effect is frequency dependent; thus, a positive feedback loop may cause the effect on the population dynamics to increase over time, with the result that the alien might eventually displace the native in a population. Effects of the examined mechanisms on population dynamics should be investigated further to improve understanding of the impact of reproductive interference on the structure of plant communities.

DOI： 10.1093/aob/mcz007

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Reproductive interference between two serious pests, oriental fruit flies Bactrocera carambolae and B. dorsalis (Diptera: Tephritidae), with very wide but partially overlapping host ranges Reviewed

Kitano D., Fujii N., Sujiono, Yamaue S., Kitamura T., Honma A., Tsukada M., Nishida T., Sawada H., Takakura K.

Applied Entomology and Zoology 53 ( 4 ) 525 - 533 2018.11

Language：English Publishing type：Research paper (scientific journal) Publisher：Applied Entomology and Zoology

© 2018, The Japanese Society of Applied Entomology and Zoology. Bactrocera carambolae and B. dorsalis (Diptera: Tephritidae) are extremely destructive pests of fruits and vegetables in the Asia-Pacific region. Earlier reports have described that B. carambolae and B. dorsalis, respectively, use mainly star fruit and mango, suggesting a certain level of host partitioning which can be ascribed neither to differences in larval food qualities nor host-specific parasitoid mortality. This study specifically examined reproductive interference (antagonistic sexual interaction) between B. carambolae and B. dorsalis as a potential factor strongly affecting their host partitioning. We observed mating behaviors, especially interspecific courtships and mating, by cohabiting the conspecific and heterospecific pairs together. Consequently, we quantified their effects on the reproductive success of females. Males of both species frequently courted their own females, but they also courted females of other species. Courtship refusal by females was not selective in males of either species. This incomplete discrimination of both sexes led to frequent occurrences of interspecific sexual interactions in both species, but only B. carambolae females showed reduced reproductive success. These results suggest that B. dorsalis, superior in reproductive interference, can occupy high-quality mango, whereas B. carambolae, inferior in reproductive interference, must use low-quality star fruit.

DOI： 10.1007/s13355-018-0584-6

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Does simultaneous and sympatric reproduction between two native spined loaches lead to reproductive interference and local extinction? Reviewed

Morii K., Nakano M., Takakura K.

Environmental Biology of Fishes 101 ( 9 ) 1407 - 1416 2018.9

Language：English Publishing type：Research paper (scientific journal) Publisher：Environmental Biology of Fishes

© 2018, Springer Nature B.V. Reproductive interference occurs when fitness of females of one species decreases because of misdirected courtship behaviors by males of another species. Reproductive interference strongly excludes either species in the interaction but it does not persist for long on an evolutionary time scale. Therefore, reproductive interference between a pair of co-evolved native species is difficult to observe because exclusion ends the interaction even if even the potential for reproductive interference still exists. However, anthropogenic environmental changes can bring about secondary contact between two species and trigger reproductive interference, leading to local species extinction. We demonstrated this phenomenon with two native spined loaches: Cobitis magnostriata (Cmag) and C. minamorii oumiensis (Cmio). In 2015 and 2016, we investigated the spatiotemporal dynamics of adults and juveniles of both species to estimate the effects of the relative abundance of adults on reproductive success. Additionally, we surveyed the distribution of juveniles and investigated the proportion of gravid females which failed to reproduce at the end of the spawning season. In 2016, Cmio juveniles were fewer than in 2015, although the abundances of Cmio adults were similarly low in both years and the Cmag adults were more abundant in 2016 than in 2015. The juveniles of the two species exhibited similar spatiotemporal dynamics. At the end of the spawning season, Cmag males were abundant and most Cmio females failed to reproduce. These results suggest that the two species share spawning habitats and seasons and that Cmag males exert reproductive interference on Cmio females. This report is the first of a study suggesting that anthropogenic environmental changes triggered reproductive interference between native species in the field.

DOI： 10.1007/s10641-018-0787-2

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Does simultaneous and sympatric reproduction between two native spined loaches lead to reproductive interference and local extinction? Reviewed

101 ( 9 ) 1407 - 1416 2018.9

Skewed male reproductive success and pollen transfer in a small fragmented population of the heterodichogamous tree Machilus thunbergii Reviewed

Watanabe S., Takakura K., Kaneko Y., Noma N., Nishida T.

Journal of Plant Research 131 ( 4 ) 623 - 631 2018.7

Language：English Publishing type：Research paper (scientific journal) Publisher：Journal of Plant Research

© 2018, The Botanical Society of Japan and Springer Japan KK, part of Springer Nature. Heterodichogamy is defined as the presence of two flower morphs that exhibit the male and female functions at different times among individuals within a population. Heterodichogamy is regarded as an adaptation to promote outcrossing through enhanced inter-morph mating, together with a 1:1 morph ratio. However, in highly fragmented populations, the morph ratio may be more likely to be biased by stochastic events. In such a situation, individuals of a minority morph within a population are expected to have higher reproductive success than those of a majority morph, which may suffer from pollen shortages of the minority morph. In this paper, we evaluated mating patterns and male reproductive success in a highly fragmented population of Machilus thunbergii, a putative heterodichogamous evergreen laurel tree. Results of paternity analysis indicated that the selfing rate was not clearly different between the two morphs. In contrast, the proportion of intra-morph mating was higher in the majority-morph (MM) mother trees than in the minority-morph (MF) mother trees. Bayesian estimated male reproductive success indicated that male reproductive success was higher in minority-morph (MF) than in majority-morph (MM) mother trees. These findings indicate that (1) the majority morph mothers, suffering a shortage of the opposite morph pollen, could partly compensate for the reduced reproductive success by intra-morph mating rather than by selfing, and (2) negative-frequency dependent selection may be involved in the maintenance of the two morphs.

DOI： 10.1007/s10265-018-1018-9

Estimation of population size of endangered plant, Arabidopsis kamchatica ssp. kawasakiana Reviewed

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Age class structure of spawning populations of two endangered endemic striated spined loaches in Lake Biwa river system. Reviewed

Morii K, Takakura KI

Japanese Journal of Environmental Entomology and Zoology 29 ( 4 ) 135 - 141 2018

Growth patterns of juveniles observed at a shared spawning site in two closely related species of spined loaches endemic to the Lake Biwa riverine system; Cobitis magnostriata and C. minamorii oumiensis Reviewed

Morii K,Nakano M, Nishida T, Takakura KI

Japanese Journal of Environmental Entomology and Zoology 2018

Unionid mussel composition and ditch environments in floodplain and alluvial fan geomorphic types: a case study of a Lake Biwa river basin Reviewed

18 ( 1 ) 41 - 49 2017.1

A Bayesian approach for estimating hexabromocyclododecane (HBCD) diastereomer compositions in water using data below limit of quantification Reviewed International journal

Ichihara M., A. Yamamoto, M. Sudo, K.I. Takakura

Environmental Science and Pollution Research 24 ( 3 ) 2667 - 2674 2017

滋賀県彦根市の水田地帯に生息するカヤネズミの食性分析－糞DNA分析からの推定 Reviewed

畠　佐代子・高倉耕一

環動昆 28 121 - 131 2017

琵琶湖につながる小規模水域におけるオオクチバスMicropterus salmoides小型個体の性成熟 Reviewed

北野大輔・曽我部共生・佐竹祐亮・高倉耕一

保全生態学研究 22 345 - 349 2017

Variation of clutch size and trophic egg proportion in a ladybird with and without male-killing bacterial infection Reviewed

30 ( 6 ) 1081 - 1095 2016.12

Spatial Distribution of Flower Color Induced by Interspecific Sexual Interaction Reviewed

Takahashi Yuma, Takakura Koh-ichi, Kawata Masakado

PLOS ONE 11 ( 10 ) 2016.10

Kosugi, A, K.-I. Takakura, N. Noma, A. Kawabe, E. Harada

38 ( 1 ) 51 - 60 2016.6

A comparison of host utilization between sibling fruit flies Bactrocera carambolae and B. papayae (Diptera: Tephritidae) in west Java, Indonesia, serious quarantine pest of fruits Reviewed

Japanese Journal of Environmental Entomology and Zoology 26 ( 4 ) 133 - 141 2016.1

Note on the invasion period of Atractomorpha sinensis (Orthoptera: Pyrgomorphidae) in Osaka, central Japan Reviewed

Kazuo Yamazaki, Koh-Ichi Takakura, Chobei Imai

Japanese journal of environmental entomology and zoology 27 ( 1 ) 17 - 20 2016

Effectiveness of common pill woodlouse, Armadillidium vulgare, as a weed control agent Reviewed

Koh-Ichi Takkaura

Japanese journal of environmental entomology and zoology 27 ( 3 ) 81 - 87 2016

Variation in the strength of reproductive interference from an alien congener to a native species in Taraxacum Reviewed International journal

Nishida S, K. Hashimoto, M. Kanaoka, K.-I. Takakura and T. Nishida

Journal of Plant Research 130 125 - 134 2016

大阪市の犬・猫・ネズミにおけるジフテリア毒素産生性Corynebacterium ulcerans の保有状況と分離株の性状 Reviewed

Umeda, K. R. Hatakeyama, T. Abe, K.-I. Takakura, T. Komiya, M. Iwaki, A. Yamamoto, H. Sanada

Journal of the Japan Veterinary Medical Association 68 ( 12 ) 765 - 769 2015.12

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japan Veterinary Medical Association

We examined the distribution of Corynebacterium ulcerans in dogs, cats and rats in Osaka city. C. ulcerans was detected in five of 137 cats (3.6 %), but not in the dogs and rats examined. The five cats from which C. ulcerans was isolated showed poor physical condition. The five isolates from the positive cats produced diphtheria toxin and showed biochemically identical, common antibiotic susceptibility patterns, genotypes for pulsed-field gel electrophoresis and ribotyping. These results indicated the possibility that genetically identical or closely related C. ulcerans is widely distributed among cats in Osaka city.

DOI： 10.12935/jvma.68.765

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Flower color polymorphism maintained by overdominant selection in Sisyrinchium sp. Reviewed

128 ( 6 ) 933 - 939 2015.11

Flower color polymorphism maintained by overdominant selection in Sisyrinchium sp. Reviewed International journal

Yuma Takahashi , Koh-ichi Takakura, Masakado Kawata

Journal of Plant Research 128 ( 6 ) 933 - 939 2015.9

Unionid freshwater mussels in irrigation ditches are affected by physical environmental factors and proximity to paddy fields Reviewed International journal

Nakano, M., T. Yamamoto, K.-I. Takakura, M. Urabe

Zoological Science 32 ( 4 ) 378 - 382 2015.8

Sexual shape dimorphism accelerated by male–male competition, but not prevented by sex-indiscriminate parental care in dung beetles (Scarabaeidae) Reviewed International journal

Shigeki Kishi,Koh-Ichi Takakura,Takayoshi Nishida

Ecology and Evolution 5 2754 - 2761 2015.7

Island biogeography as a test of reproductive interference Reviewed International journal

http://link.springer.com/article/10.1007%2Fs10144-015-0489-y

Population Ecology 57 307 - 309 2015.7

Conflicting intersexual mate choices maintain interspecific sexual interactions Reviewed International journal

Koh-Ichi Takakura, Takayoshi Nishida, Keisuke Iwao

Population Ecology 57 ( 2 ) 261 - 271 2015.7

Host specialization by reproductive interference between closely related herbivorous insects Reviewed International journal

Takayoshi Nishida,Kohichi Takakura,Keisuke Iwao

Population Ecology 57 ( 2 ) 273 - 281 2015.7

Evaluation of seed banks for restoration of wetland vegetation: an integrated assessment of the seedling establishment and direct counting methods based on a Bayesian model Reviewed

Bulletin of Kansai Organization for Nature Conservation 37 ( 1 ) 47 - 59 2015.6

Reproductive Interference between the native and the alien dandelion (Taraxacum) in Izu, Japan Reviewed

15 ( 1 ) 41 - 50 2015.5

Conflicting intersexual mate choices maintain interspecific sexual interactions Reviewed

57 ( 2 ) 261 - 271 2015.4

Host specialization by reproductive interference between closely related herbivorous insects Reviewed

57 ( 2 ) 273 - 281 2015.4

Island biogeography as a test of reproductive interference Reviewed

57 ( 2 ) 307 - 319 2015.4

Associations between Co-Detected Respiratory Viruses in Children with Acute Respiratory Infections Reviewed

67 ( 6 ) 469 - 475 2014.11

Impacts of environmental factors in rice paddy fields on abundance of the mud snail (Cipangopaludina chinensis laeta) Reviewed

80 460 - 463 2014.11

Distribution of Capnocytophaga canimorsus in dogs and cats with genetic characterization of isolates Reviewed

171 ( 1-2 ) 153 - 159 2014.6

Pollen–pistil interactions in reproductive interference: comparisons of heterospecific pollen tube growth from alien species between two native Taraxacum species Reviewed

Nishida S, MM Kanaoka, K Hashimoto, KI Takakura, T Nishida

Functional Ecology 28 450 - 457 2014.4

Pollen-pistil interactions in reproductive interference: comparisons of heterospecific pollen tube growth from alien species between two native Taraxacum species Reviewed

Sachiko Nishida, Masahiro M. Kanaoka, Keisuke Hashimoto, Koh-Ichi Takakura, Takayoshi Nishida

FUNCTIONAL ECOLOGY 28 ( 2 ) 450 - 457 2014.4

Language：English Publishing type：Research paper (scientific journal) Publisher：WILEY-BLACKWELL

1. Reproductive interference (RI), any negative interspecific interaction during the reproductive process, has been gaining increasing attention due to its potential explanatory power for the mutually exclusive distribution of closely related species. RI in plants may occur during any of three stages: pollen transfer, pollen-pistil interactions or hybridization. Pollen-pistil interactions may be especially important as most studies of RI have suggested the involvement at this stage. Details of these interactions are required to fully explore RI and are especially relevant in considering the impact of RI in the field.
2. We present a plausible explanation of how RI functions in the pollen-pistil interaction stage using two Japanese native dandelions, Taraxacum japonicum and Taraxacum longeappendiculatum, of which only the former is vulnerable to RI from an alien congener, Taraxacum officinale. We conducted a series of hand pollinations in these native dandelions and compared pollen tube behaviour to examine differences associated with vulnerability and imperviousness to RI from the alien.
3. The two native dandelions differed in terms of the absence/presence of pollen tube elongation after heterospecific pollination (pollination with only T. officinale): pollen tubes grew through the ovaries of the vulnerable T. japonicum, but not through those of the impervious T. longeappendiculatum. In vitro hand pollination verified that the alien pollen tubes could extend into the ovaries of T. japonicum.
4. Our results show that RI from the alien dandelion consumed ovules by heterospecific pollen deposition. The pistils of the impervious native species could prevent growth of the alien pollen tubes, thereby sparing the ovules for fertilization by conspecific pollen. The pistils of the vulnerable species lacked interspecific incompatibility against the alien, and thus, the alien pollen tube entered the ovary, eliminating an opportunity for conspecific pollen fertilization. This consumption of ovules by heterospecific pollen tubes would cause a seed set failure, leading to reduced abundance and a further exertion of RI in the next generation, which explains displacement of the vulnerable species by the alien in the field.

DOI： 10.1111/1365-2435.12165

Impacts of environmental factors in rice paddy fields on abundance of the mud snail (Cipangopaludina chinensis laeta) Reviewed

Kosuke Nakanishi, Koh-Ichi Takakura, Ryosuke Kanai, Kota Tawa, Daisuke Murakami, Hiroichi Sawada

Journal of Molluscan Studies 80 460 - 463 2014

Frequent respiratory viral infections in a young child in a 27-month follow-up study Reviewed

Atsushi Kaida, Hideyuki Kubo, Nobuhiro Iritani, Seiji P. Yamamoto, Atsushi Hase, Koh-Ichi Takakura, Tsutomu Kageyema

JMM Case Reports in press 2014

Reproductive population dynamics of the Far Eastern Catfish, Silurus asotus estimated from mark and recapture and Bayesian modeling Reviewed

TAKAKURA Koh-ichi

Funao, T., K.I. Takakura, T. Nishida, H. Sawada 61 ( 1 ) 15 - 26 2014

Biofilm Formation and Resistance to Benzalkonium Chloride in Listeria monocytogenes Isolated from a Fish Processing Plant Reviewed

Hiromi Nakamura, Koh-Ichi Takakura, Yoshiaki Sone, Yasuyuki Itano, Yoshikazu Nishikawa

JOURNAL OF FOOD PROTECTION 76 ( 7 ) 1179 - 1186 2013.7

Language：English Publishing type：Research paper (scientific journal) Publisher：INT ASSOC FOOD PROTECTION

Listeria monocytogenes is a foodborne pathogen that causes the potentially life-threatening illness listeriosis. Previously, a few clones of L. monocytogenes persisting in a cold-smoked fish processing plant were isolated from the plant's products continuously. To evaluate the role of biofilms in the persistence of L. monocytogenes strains specific to this plant, the abilities of the persistent strain (PS) and transient strain (TS) of L. monocytogenes found in this plant to form biofilms were compared, as was resistance to the sanitizing effects of benzalkonium chloride (BC). The PS produced more biofilm than the TS in 48 h. The half-maximal effective concentration (EC50), the BC concentration at which the ATP bioluminescence of each bacterial strain decreased by 50% relative to its maximum activity, was about 150-fdld higher in the PS than in the TS. In contrast, when these values were measured in organisms in a planktonic state, the EC50 of the PS was only 2.2-fold higher than that of the TS. Extracellular polymeric substances (EPS) were extracted from biofilms, and the glucose content of these biofilms was determined with the phenol sulfuric acid method to estimate the quantity of EPS. The total amount of EPS in the PS biofilm was higher than that in the TS biofilm. These findings suggest that the PS produces greater amounts of biofilm and EPS than the TS, which results in greater resistance of the PS to disinfectants. The persistence of the strain in the fish processing plant might he attributable to these properties.

DOI： 10.4315/0362-028X.JFP-12-225

Analyses and predictions of the presence and reproduction of three water birds at ponds in Osaka Prefecture with consideration of spatial auto-correlation Reviewed

Koh-ichi Takakura, Takayuki Nishio, Masaaki Kitano

ORNITHOLOGICAL SCIENCE 12 ( 1 ) 15 - 26 2013.6

Language：English Publishing type：Research paper (scientific journal) Publisher：ORNITHOLOGICAL SOC JAPAN, UNIV TOKYO, SCH AGR

We analyzed existing records of the presence and breeding of three water bird species (Little Grebe Tachybaptus ruficollis; Common Moorhen Gallinula chloropus; and Spot-billed Duck Anas zonorhyncha) at 1,087 ponds in Osaka Prefecture to estimate the effects on them of various environmental, geographical, and social factors. A conditional autoregressive (CAR) model, incorporating spatial structure as a random effect, was applied in the analyses. Results show a significant positive effect of a broad shape of the water surface, which indicates the importance of threats by terrestrial enemies, on the probability of the presence of each species. Two human social factors affected the probability of breeding of two bird species: human population density negatively affected the breeding probability of T ruficollis and A. zonorhyncha; and the retail store density, an index of development as a commercial area, positively affected the latter species. Spatial random effects reflecting unmeasured factors were large and comparable to other measured factors. Different patterns were exhibited among the three species, demonstrating that other important factors were overlooked and that these effects should be assessed by application of methods such as the CAR model.

DOI： 10.2326/osj.12.15

Dominant occurrence of cleistogamous flowers of Lamium amplexicaule in relation to the nearby presence of an alien congener L. purpureum (Lamiaceae) Reviewed

Occurrence of fluoroquinolones and fluoroquinolone-resistance genes in the aquatic environment Reviewed

444 508 - 514 2013.2

Two-Way but Asymmetrical Reproductive Interference between an Invasive Veronica Species and a Native Congener Reviewed

Koh-Ichi Takakura

American Journal of Plant Sciences 2013 ( 4 ) 535 - 542 2013

Yasuhiro Sato, Koh-Ichi Takakura, Sachiko Nishida, Takayoshi Nishida

ISRN Ecology 2013 Article ID 476862 - 6 2013

Language：English Publishing type：Research paper (scientific journal) Publisher：Hindawi Limited

Here we document a novel phenomenon that, based on field observations in central Japan, cleistogamous flowers (or closed flowers) of an annual herb <italic>Lamium amplexicaule</italic> were dominantly expressed near an alien congener <italic>L. purpureum</italic>. The proportion of cleistogamous flowers in an individual <italic>L. amplexicaule</italic> increased with the frequency of <italic>L. purpureum</italic> occurring in the same patches but did not increase with the total density of <italic>Lamium</italic> plants and their own size. To confirm the consistency of the effect of the coexisting alien species, we assessed the cleistogamous frequency at the patch level for three other populations. In these populations as well, the proportion of <italic>L. amplexicaule</italic> producing cleistogamous flowers increased with the frequency of <italic>L. purpureum</italic>. Our transplant experiment at one site found no effect of the nearby presence of <italic>L. purpureum</italic> on the seed set of <italic>L. amplexicaule</italic> and therefore did not support the hypothesis that the adverse effect on the reproduction via interspecific pollination favored cleistogamous flowers that accepted no external pollen. Further studies must be conducted to examine the negative interactions between the related species before and after seed development.

DOI： 10.1155/2013/476862

Other Link： http://downloads.hindawi.com/archive/2013/476862.xml

Bayesian estimation of population dynamics of feral cats in Osaka City, Japan Reviewed

Koh-Ichi Takakura, Takuto Abe, Kazuhide Doi, Shu-Ichi Sanada, Atsushi Hase

Japanese Journal of Environmental Entomology and Zoology in press ( 4 ) 133 - 141 2013

Safer DNA extraction from plant tissues using sucrose buffer and glass fiber filter Reviewed

Koh-Ichi Takakura, Takayuki Nishio

JOURNAL OF PLANT RESEARCH 125 ( 6 ) 805 - 807 2012.11

Language：English Publishing type：Research paper (scientific journal) Publisher：SPRINGER JAPAN KK

For some plant species, DNA extraction and downstream experiments are inhibited by various chemicals such as polysaccharides and polyphenols. This short communication proposed an organic-solvent free (except for ethanol) extraction method. This method consists of an initial washing step with STE buffer (0.25 M sucrose, 0.03 M Tris, 0.05 M EDTA), followed by DNA extraction using a piece of glass fiber filter. The advantages of this method are its safety and low cost. The purity of the DNA solution obtained using this method is not necessarily as high as that obtained using the STE/CTAB method, but it is sufficient for PCR experiments. These points were demonstrated empirically with two species, Japanese speedwell and common dandelion, for which DNA has proven difficult to amplify via PCR in past studies.

DOI： 10.1007/s10265-012-0502-x

Bayesian Estimation for the Effectiveness of Pesticides and Repellents Reviewed

K. I. Takakura

JOURNAL OF ECONOMIC ENTOMOLOGY 105 ( 5 ) 1856 - 1862 2012.10

Language：English Publishing type：Research paper (scientific journal) Publisher：ENTOMOLOGICAL SOC AMER

In the quantitative estimation of the effectiveness of pesticides and repellents, Abbott's correction and similar methods have been used for nearly a century. However, the formulas for such corrections have some disadvantages in performing range estimations and being used in batched experiments. These disadvantages also exist in the estimation of EC50, the chemical concentration that produces 50% mortality. In this study, I propose as a solution to these problems a Bayesian estimation with a logistic model. This method enables the flexible modeling of non-normal variables in complex experimental design, for example, life-death response in a batched experiment. Furthermore, if any knowledge about focal phenomena exists, it can be used in analyses as a prior distribution, thus enhancing the accuracy of the estimation.

DOI： 10.1603/EC11360

Increase of GII.2 norovirus infections during the 2009-2010 season in Osaka City, Japan Reviewed

84 ( 3 ) 517 - 525 2012.3

Differential effects of reproductive interference by an alien congener on native Taraxacum species Reviewed

Sachiko Nishida, Koh-Ichi Takakura, Takayoshi Nishida, Takashi Matsumoto, Masahiro M. Kanaoka

BIOLOGICAL INVASIONS 14 ( 2 ) 439 - 447 2012.2

Language：English Publishing type：Research paper (scientific journal) Publisher：SPRINGER

Reproductive interference (RI) has been suggested to play a critical role in native plant displacement by alien congeners. However, although co-existence of native and alien congeners may provide an opportunity to refute the RI hypothesis, few studies have examined such a case. Using a native Japanese dandelion, Taraxacum longeappendiculatum, and a co-existing alien congener, Taraxacum officinale, we tested the hypothesis that differences in RI by the alien between native recipient congeners explain whether a native will co-exist with or be displaced by an alien. We conducted a field survey to investigate the effects of alien relative abundance on T. longeappendiculatum seed set, and a hand-pollination experiment to identify the extent of pollen interference by the alien on T. longeappendiculatum. We compared these results with those obtained previously for another Japanese native species, Taraxacum japonicum, which was displaced by the alien. In our field survey, alien relative abundance had little effect on seed set in nearby T. longeappendiculatum, and hand-pollination with mixed pollen grains produced no substantial decrease in seed set of the native species. Model selection supported these tendencies; the effect of RI by the alien differed between the two native species. Other potential factors, such as resource competition or habitat changes, could not explain the co-existence of T. longeappendiculatum with and exclusion of T. japonicum by the alien in the same explanatory framework. Considering the consistent explanatory power, the findings suggest that RI is a critical mechanism that can determine both co-existence with and displacement of native dandelions by an alien congener.

DOI： 10.1007/s10530-011-0088-6

A simple criterion for distinguishing between sympatric fruit flies Bactrocera carambolae and B. papayae (Diptera: Tephritidae) notorious pests of diverse fruits on Java Reviewed

Hiroshi Kago, Hiroichi Sawada, Agus Susanto, Koh-ichi Takakura, Takayoshi Nishida, Morio Tsukada

Journal of Environmental Entomology and Zoology 23 ( 3 ) 143 - 149 2012

Analyses of the reproductive interference between native and invasive dandelions with individual based model(<Feature 2>Reproductive interference and ecological communities) Reviewed

Takakura Koh-Ichi, Matsumoto Takashi, Nishida Sachiko, Nishida Takayoshi

JAPANESE JOURNAL OF ECOLOGY 62 ( 2 ) 255 - 265 2012

No Reproductive Interference from an Alien to a Native Species in Cerastium (Caryophyllaceae) at the Stage of Seed Production Reviewed

Koh-Ichi Takakura

ISRN Botany 2012 ID 193807 2012

High Incidence of Human Echovirus Type 3 among Children in Osaka, Japan during the Summer of 2010 Reviewed

64 ( 6 ) 533 - 535 2011.11

Molecular Epidemiology of Human Rhinovirus C in Patients with Acute Respiratory Tract Infections in Osaka City, Japan Reviewed

64 ( 6 ) 488 - 492 2011.11

Improved method of plant DNA extraction using glass-fiber filter

Koh-ichi Takakura

Bunrui 11 ( 2 ) 139 - 149 2011.8

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：Japanese Society for Plant Systematics

In recent years, molecular biological analyses have been used in diverse areas of biology study. This wider use has increased demand for more convenient and safe methods of DNA extraction and purification. The author proposes an improved DNA extraction method using a glass-fiber filter. The improvements were mainly three. The first was centrifugation of homogenized plant tissue. The second was the addition of polyvinylpolypyrrolidone (PVPP) to the extraction reagent as a polyphenol absorbent. The final was the removal and collection of solvent from the glass fiber filter pieces using a desktop centrifuge. This improved method, which can extract sufficiently pure DNA solution for PCR amplification in diverse plant species, presents advantages over many conventional methods such as lower cost and higher safety, while accommodating a wider range of applications than the conventional glass fiber filter method can.

DOI： 10.18942/bunrui.KJ00007405277

イヌノフグリの“多型”－石垣環境への適応と種子散布者との関係－

A tortricid moth of Cydia sp (Lepidoptera: Tortricidae) boring into thorns of Gleditsia japonica Miq. (Fabaceae) in Japan

14 ( 2 ) 179 - 181 2011.6

A tortricid moth of Cydia sp. (Lepidoptera: Tortricidae) boring into thorns of Gleditsia japonica Miq. (Fabaceae) in Japan Reviewed

14 ( 2 ) 179 - 181 2011.6

Effective range of reproductive interference exerted by an alien dandelion, Taraxacum officinale, on a native congener

124 ( 2 ) 269 - 276 2011.3

Alien Dandelions Displace a Native Related Species Through Interspecific Pollen Transfer Reviewed

83 - + 2011

高倉耕一, 西田佐知子, 西田隆義

日本生態学会関東地区会会報 59 19 - 25 2011

大阪市域における特定外来生物の分布:インターネット上の情報活用の可能性と課題

高倉耕一

大阪市立環境科学研究所報告調査研究年報 73 65 - 73 2011

大阪市万代池公園の動物相

山崎一夫, 高倉耕一

大阪市立環境科学研究所報告調査研究年報 73 75 - 87 2011

Separation of diurnal and seasonal variation from urban ozone monitoring by Bayesian statistics

Yasuyuki Itano, Koh-ichi Takakura

Journal of Japan Society for Atmospheric Environment / Taiki Kankyo Gakkaishi 46 ( 3 ) 179 - 186 2011

Reproductive interference and its implications for the biogeography and ecology of plants

TAKAKURA Koh-Ichi, NISHIDA Sachiko, NISHIDA Takayoshi

bunrui 10 ( 2 ) 151 - 162 2010.8

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：日本植物分類学会

Reproductive interference (RI) refers to negative interspecific interactions in which the reproductive activities of one species directly reduce the reproductive success of another species. RI can be observed in various events in plant reproductive processes, such as stigma clogging and pollen allelopathy. The most conspicuous feature of RI is its positive frequency dependence and its self-reinforcing impact via positive feedback: when two species exert RI on one another, the more abundant species exerts a more intense adverse effect on the reproductive success of the other and then becomes...

DOI： 10.18942/bunrui.KJ00006537116

If they have no leaves, let them eat bread? : A remarkable food habit in the cotton bollworm Helicoverpa armigera (Hubner) (Lepidoptera, Noctuidae)

YAMAZAKI Kazuo, TAKAKURA Koh-ichi, IMAI Chobei

Transactions of the Lepidopterological Society of Japan 61 ( 2 ) 173 - 175 2010.7

Language：English Publisher：The Lepidopterological Society of Japan

A late-instar larva of the cotton bollworm Helicoverpa armigera (Hubner) was found feeding on bread in a residential home in Osaka. The larva may have inadvertently invaded the house, or earlier the grocery store or bakery, to ultimately feed on the bread. The larva was subsequently reared on the bread in the laboratory, but it died before pupation. This occurrence represents a rare case of the infestation of a processed cereal food by a macrolepidopteran caterpillar and the second report of bread-feeding in the cotton bollworm. Helicoverpa armigera caterpillars typically feed on leaves and...

DOI： 10.18984/lepid.61.2_173

Procedure and prospects of biodiversity conservation in urban areas

Alien pollen grains interfere with the reproductive success of native congener

12 ( 6 ) 1617 - 1626 2010.6

Detection and quantitative analysis of human bocavirus associated with respiratory tract infection in Osaka City, Japan

54 ( 5 ) 276 - 281 2010.5

Reproductive interference and salinity tolerance differentiate habitat use between two alien cockleburs: Xanthium occidentale and X. italicum (Compositae)

206 ( 2 ) 309 - 319 2010.2

If they have no leaves, let them eat bread? - A remarkable food habit in the cotton bollworm Helicoverpa armigera (Hübner) (Lepidoptera, Noctuidae)

Yamazaki, K, Takakura, K.-I, Imai, C

Transactions of the Lepidopterological Society of Japan 61 ( 2 ) 173 - 175 2010

Journal of Urban Living and Health Association 54 ( 2 ) 85 - 92 2010

大阪府におけるオナモミ類の変遷（その２）

藤井伸二, 高倉耕一

Nature Study 56 ( 11 ) 2 - 5 2010

大阪府におけるオナモミ類の変遷（その１）

藤井伸二, 高倉耕一

Nature Study 56 ( 10 ) 6 - 8 2010

Surveillance of Mosquitoes for West Nile Virus in Osaka City from 2005 to 2009

Kaoru GOTOH, Atsushi KAIDA, Hideyuki KUBO, Niichiro ABE, Nobuhiro IRITANI

73 7 - 14 2010

Time-dependent change in symptoms of sick house syndrome and in indoor environment of patient dwellings

Journal of Urban Living and Health Association 54 ( 4 ) 312 - 320 2010

Reproductive interference as a mechanism benefiting the successful invasion of alien species: Case studies with dandelions and cockleburs.

Takakura Koh-ichi

31 ( 2 ) 119 - 135 2009.12

Reconsiderations on Evaluating Methodology of Repellent Effects: Validation of Indices and Statistical Analyses

102 ( 5 ) 1977 - 1984 2009.10

Alien dandelion reduces the seed-set of a native congener through frequency-dependent and one-sided effects

11 ( 4 ) 973 - 981 2009.4

Evolutionary ecology and control of infectious disease virulence

Journal of Urban Living and Health Association 53 ( 3 ) 145 - 152 2009

Optimal-Foraging Predator Favors Commensalistic Batesian Mimicry Reviewed

3 ( 10 ) e3411 2008.10

Temporal change in the density and feeding habits of a terrestrial red mite, Balaustium murorum (Hermann), on a building roof

Koh-ichi Takakura, Ayato Kohzu

JAPANESE JOURNAL OF APPLIED ENTOMOLOGY AND ZOOLOGY 52 ( 2 ) 87 - 93 2008

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：JAPAN SOC APPL ENTOMOL ZOOL

We observed the seasonal occurrence and foraging behaviors of a terrestrial red mite, Balaustium murorum (Hermann), on the roof of a building in Osaka and analyzed the stable isotope ratios in the mite and three prey species to study its feeding habits. On direct observation, the adult mites formed groups and fed on small insects and anthers that had fallen on the roof. When pollen of the grass Polypogon fugax Steud. was experimentally added to the roof surface, the adult mites fed more frequently than they did without the pollen. We measured the stable isotope ratios of C and N in the mite and three prey species, P. fugax, a plant louse (Psyliidae sp.), and a cyanobacterium (Hassallia sp.), and estimated the contribution of each to the mite. This showed that P.fugax made the largest contribution, about 50%, irrespective of the estimation method. From these results, we concluded that the pollen was the most important food resource for the mite, although it is an omnivore.

DOI： 10.1303/jjaez.2008.87

New applications of computers in statistical analysis: randomized test and Monte Carlo method

都市の生物相--セミをめぐる研究から

高倉耕一

生活衛生 52 ( 4 ) 239 - 244 2008

Journal of Urban Living and Health Association 52 ( 4 ) 221 - 228 2008

大阪およびその周辺地域に優占する外来巻貝ハブタエモノアラガイLymnaea columella (Say)とその自家受精による繁殖能力

高倉耕一

大阪市立環境科学研究所報告 70 43 - 51 2008

Cover dependence of predation avoidance alters the effect of habitat fragmentation on two cicadas (Hemiptera : Cicadidae)

K. I. Takakura, K. Yamazaki

ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA 100 ( 5 ) 729 - 735 2007.9

Language：English Publishing type：Research paper (scientific journal) Publisher：ENTOMOLOGICAL SOC AMER

Habitat fragmentation increases habitat edge, which often enhances predation pressure. This phenomenon, termed the "edge effect," has been documented for many organisms. However, its underlying mechanisms are not sufficiently understood, particularly for insect species. Thus, we aimed to interpret the behavioral mechanism of edge effects in two cicada species: Graptopsaltria nigrofuscata (Motschulsky) and Cryptotympana facialis (Walker) (Hemiptera: Cicadidae). In recent decades, the G. nigrofuscata has been decreasing in urban environments, whereas C. facialis has not. We compared the predation pressure by birds in four urban and three suburban habitats and we observed predation-avoidance behaviors. Results showed that the G. nigrofuscata incurred extremely high predation pressure. However, in C. facialis, predation pressure was constant among habitats. Behavioral observations revealed that predation avoidance of G. nigrofuscata was highly cover dependent, because threatened individuals hid in nearby trees. When tree cover is highly fragmented, the G. nigrofuscata probably has difficulty hiding. However, C. facialis fled cover independently. Front these results, we concluded that the main causes of the decline of G. nigrofuscata in urban habitats were its cover dependence and the increasing discontinuity of tree coverage.

DOI： 10.1603/0013-8746(2007)100[729:CDOPAA]2.0.CO;2