2024/01/09 更新

写真a

ヒガシダ カズヒコ
東田  一彦
HIGASHIDA Kazuhiko
所属
人間文化学研究院
部局
人間文化学部 生活栄養学科
職名
准教授
外部リンク

学歴

  • 早稲田大学   スポーツ科学研究科

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    修了課程: 博士課程

    国名: 日本国

  • 早稲田大学スポーツ科学研究科 修士課程

  • 早稲田大学スポーツ科学研究科 博士後期課程

学位

  • 博士(スポーツ科学) ( 2010年3月   早稲田大学 )

研究キーワード

  • 運動生理学

  • スポーツ栄養学

経歴

  • 滋賀県立大学   人間文化学部 人間文化学部生活文化学科・食生活専攻   准教授

    2015年4月 - 現在

  • 滋賀県立大学   人間文化学部 生活栄養学科   准教授

    2015年4月 - 現在

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    国名:日本国

  • 早稲田大学 スポーツ科学学術院   助教

    2014年4月 - 2015年3月

  • 早稲田大学スポーツ科学学術院   助手

    2013年4月 - 2014年3月

  • 立命館大学 総合科学技術研究機構   ポスドク

    2012年4月 - 2013年3月

  • Washington University in St. Louis   School of Medicine   ポスドク

    2011年5月 - 2012年3月

  • 日本学術振興会 特別研究員   ポスドク

    2010年4月 - 2011年4月

▼全件表示

所属学協会

  • American physiological Society

    2020年4月 - 現在

  • 日本アミノ酸学会

    2015年4月 - 現在

  • 日本スポーツ栄養学会

    2014年4月 - 現在

  • 日本栄養・食糧学会

    2012年4月 - 現在

  • 日本体力医学会

    2006年4月 - 現在

研究分野

  • ライフサイエンス / スポーツ科学

  • ライフサイエンス / 栄養学、健康科学

  • ライフサイエンス / スポーツ科学

研究シーズ

  • 身体運動によるエネルギー代謝亢進機序に関する研究

論文

  • Interaction Between Sarcopenic Obesity and Nonlocomotive Physical Activity on the Risk of Depressive Symptoms in Community-Dwelling Older Adult Japanese Women 査読

    Yu Osugi, Aiko Imai, Toshiyuki Kurihara, Keiko Kishigami, Kazuhiko Higashida, Kiyoshi Sanada

    Journal of Aging and Physical Activity   17   1 - 17   2023年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   参加形態:共同(副担当)  

  • Low-carbohydrate, high-fat diet, and running exercise influence bone parameters in old mice. 国際誌

    Yuki Aikawa, Takenori Yamashita, Naoya Nakai, Kazuhiko Higashida

    Journal of applied physiology (Bethesda, Md. : 1985)   132 ( 5 )   1204 - 1212   2022年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We examined the effects and interactions of a low-carbohydrate, high-fat (LCHF) diet and voluntary running exercise on bone in older mice. Male 19-mo-old mice were divided into four groups by diet (control vs. LCHF) and exercise (sedentary vs. voluntary running). The control diet was 55% carbohydrate, 23% protein, and 22% fat, and the LCHF diet was 10% carbohydrate, 33% protein, and 57% fat as percentages of calories. The experiment ended when the mice reached 24 mo old. Statistical analysis was conducted using two-way analysis of variance with diet and exercise. The LCHF diet decreased bone mineral content (BMC), bone mineral density, bone volume fraction, and trabecular number. There was no significant interaction between diet and exercise on many bone parameters. However, there were significant diet and exercise interactions on lumbar BMC and tibial trabecular total tissue volume and average cortical thickness. The LCHF diet attenuated the benefit of running exercise on lumbar BMC and caused running to have a negative effect on tibial trabecular total tissue volume. Our study suggests that the LCHF diet impairs bone mass and some trabecular microstructure and reduces the benefit of exercise on lumbar BMC in old mice.NEW & NOTEWORTHY An LCHF diet is used in treatment and prevention of diseases or improving exercise performance. However, some studies have shown that an LCHF diet diminishes bone in young rodents. Our study demonstrates that an LCHF diet impairs bone mass and some trabecular microstructure in old mice, which are similar to the previous studies using young rodents. Moreover, our study shows that an LCHF diet reduces the benefit of exercise on lumbar BMC in old mice.

    DOI: 10.1152/japplphysiol.00789.2021

    PubMed

  • BDK knockout skeletal muscle satellite cells exhibit enhanced protein translation initiation signal in response to BCAA in vitro 査読

    Nakai N, Iida N, Kitai S, Shimomura Y, Kitaura Y, Higashida K

    Biosci Biotechnol Biochem   Zbac021   2022年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   参加形態:共同(副担当)  

  • Basal and resistance exercise-induced increase in protein synthesis is impaired in skeletal muscle of iron-deficient rats 査読

    Higashida K, Inoue S, Takeuchi N, Ato S, Ogasawara R, Nakai N.

    Nutrition   91-92   2021年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   参加形態:共同(主担当)  

    DOI: 10.1016/j.nut.2021.111389

  • c-Myc overexpression increases ribosome biogenesis and protein synthesis independent of mTORC1 activation in mouse skeletal muscle 査読

    Mori T, Ato S, Knudsen JR, Henriquez-Olguin C, Li Z, Wakabayashi K, Suginohara T, Higashida K, Tamura Y, Nakazato K, Jensen TE, Ogasawara R.

    Am J Physiol Endocrinol Metab   321 ( 4 )   551 - 559   2021年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   参加形態:共同(副担当)  

  • Glucose enhances catecholamine-stimulated lipolysis via increased glycerol-3-phosphate synthesis in 3T3-L1 adipocytes and rat adipose tissue 査読

    Takeuchi N, Higashida K, Li X, Nakai N.

    Mol Biol Rep   48 ( 6 )   6269 - 6276   2021年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   参加形態:共同(副担当)  

  • c-Myc overexpression increases ribosome biogenesis and protein synthesis independent of mTORC1 activation in mouse skeletal muscle. 国際誌

    Takahiro Mori, Satoru Ato, Jonas R Knudsen, Carlos Henriquez-Olguin, Zhencheng Li, Koki Wakabayashi, Takeshi Suginohara, Kazuhiko Higashida, Yuki Tamura, Koichi Nakazato, Thomas E Jensen, Riki Ogasawara

    American journal of physiology. Endocrinology and metabolism   321 ( 4 )   E551-E559   2021年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    High-intensity muscle contractions (HiMC) are known to increase c-Myc expression which is known to stimulate ribosome biogenesis and protein synthesis in most cells. However, while c-Myc mRNA transcription and c-Myc mRNA translation have been shown to be upregulated following resistance exercise concomitantly with increased ribosome biogenesis, this has not been tested directly. We investigated the effect of adeno-associated virus (AAV)-mediated c-Myc overexpression, with or without fasting or percutaneous electrical stimulation-induced HiMC, on ribosome biogenesis and protein synthesis in adult mouse skeletal muscles. AAV-mediated overexpression of c-Myc in mouse skeletal muscles for 2 weeks increased the DNA polymerase subunit POL1 mRNA, 45S-pre-rRNA, total RNA, and muscle protein synthesis without altering mechanistic target of rapamycin complex 1 (mTORC1) signaling under both ad libitum and fasted conditions. RNA-seq analyses revealed that c-Myc overexpression mainly regulated ribosome biogenesis-related biological processes. The protein synthesis response to c-Myc overexpression mirrored the response with HiMC. No additional effect of combining c-Myc overexpression and HiMC was observed. Our results suggest that c-Myc overexpression is sufficient to stimulate skeletal muscle ribosome biogenesis and protein synthesis without activation of mTORC1. Therefore, the HiMC-induced increase in c-Myc may contribute to ribosome biogenesis and increased protein synthesis following HiMC.

    DOI: 10.1152/ajpendo.00164.2021

    PubMed

  • Basal and resistance exercise-induced increase in protein synthesis is impaired in skeletal muscle of iron-deficient rats

    Kazuhiko Higashida, Sachika Inou, Nodoka Takeuchi, Satoru Ato, Riki Ogasawara, Naoya Nakai

    Nutrition   111389 - 111389   2021年6月

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    掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier BV  

    DOI: 10.1016/j.nut.2021.111389

  • Ketogenic diet feeding improves aerobic metabolism property in extensor digitorum longus muscle of sedentary male rats 査読

    Ogura Y, Kakehashi C, Yoshihara T, Kurosaka M, Kakigi R, Higashida K, Fujiwara SE, Akema T, Funabashi T.

    Plos one   2020年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   参加形態:共同(副担当)  

    DOI: 10.1371/journal.pone.0241382. eCollection 2020.

  • Iron deficiency attenuates protein synthesis stimulated by branched-chain amino acids and insulin in myotubes. 国際誌

    Kazuhiko Higashida, Sachika Inoue, Naoya Nakai

    Biochemical and biophysical research communications   531 ( 2 )   112 - 117   2020年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Iron deficiency anemia indicates poor nutrition and is a public health problem. Iron deficiency is also associated with muscle weakness. However, the intracellular mechanisms by which iron deficiency induces muscle weakness are obscure. The purpose of the present study was to evaluate the effect of iron deficiency on protein synthesis in basal and branched-amino acids (BCAA)- and insulin-stimulated state in muscle cells. Differentiated C2C12 myotubes were incubated with an iron chelator, deferoxamine mesylate, and then stimulated with BCAA or insulin to activate protein synthesis. This iron deprivation resulted in a significant reduction in the abundance of iron-containing proteins, such as the mitochondrial complex 1 subunit protein, compared to control cells, but not of protein that does not contain iron, such as citrate synthase. Proteins involved in glucose utilization, such as glucose transpoter-1, hexokinase and AMP-activated protein kinase (AMPK), were upregulated under iron deficiency. Additionally, rates of BCAA- and insulin-stimulated protein synthesis, measured by puromycin incorporation, were lower in iron-deficient myotubes than in control cells. We suggest that low iron availability attenuates BCAA- and insulin-stimulated protein synthesis, possibly via activation of AMPK in myotubes. The present findings advance the understanding of the importance of iron to skeletal muscle protein synthesis and, thus, may contribute to the prevention of sarcopenia and frailty.

    DOI: 10.1016/j.bbrc.2020.07.041

    PubMed

  • Autophagy under glucose starvation enhances protein translation initiation in response to re-addition of glucose in C2C12 myotubes. 国際誌

    10 ( 10 )   2149 - 2156   2020年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1002/2211-5463.12970

    PubMed

  • Iron deficiency attenuates protein synthesis stimulated by branched-chain amino acids and insulin in myotubes 査読

    Higashida K., Inoue S., Nakai N.

    Biochemical and Biophysical Research Communications   2020年8月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Biochemical and Biophysical Research Communications  

    © 2020 Elsevier Inc. Iron deficiency anemia indicates poor nutrition and is a public health problem. Iron deficiency is also associated with muscle weakness. However, the intracellular mechanisms by which iron deficiency induces muscle weakness are obscure. The purpose of the present study was to evaluate the effect of iron deficiency on protein synthesis in basal and branched-amino acids (BCAA)- and insulin-stimulated state in muscle cells. Differentiated C2C12 myotubes were incubated with an iron chelator, deferoxamine mesylate, and then stimulated with BCAA or insulin to activate protein synthesis. This iron deprivation resulted in a significant reduction in the abundance of iron-containing proteins, such as the mitochondrial complex 1 subunit protein, compared to control cells, but not of protein that does not contain iron, such as citrate synthase. Proteins involved in glucose utilization, such as glucose transpoter-1, hexokinase and AMP-activated protein kinase (AMPK), were upregulated under iron deficiency. Additionally, rates of BCAA- and insulin-stimulated protein synthesis, measured by puromycin incorporation, were lower in iron-deficient myotubes than in control cells. We suggest that low iron availability attenuates BCAA- and insulin-stimulated protein synthesis, possibly via activation of AMPK in myotubes. The present findings advance the understanding of the importance of iron to skeletal muscle protein synthesis and, thus, may contribute to the prevention of sarcopenia and frailty.

    DOI: 10.1016/j.bbrc.2020.07.041

    Scopus

    その他リンク: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85089189302&origin=inward

  • Iron deficiency attenuates catecholamine-stimulated lipolysis via downregulation of lipolysis-related proteins and glucose utilization in 3T3-L1 adipocytes 査読 国際誌

    Higashida K, Takeuchi N, Inoue N, Hashimoto T, Nakai N

    Molecular Medicine Reports   2020年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Iron deficiency attenuates catecholamine‑stimulated lipolysis via downregulation of lipolysis‑related proteins and glucose utilization in 3T3‑L1 adipocytes. 国際誌

    21 ( 3 )   1383 - 1389   2020年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3892/mmr.2020.10929

    PubMed

  • Application of Molecular Hydrogen as a Novel Antioxidant in Sports Science 査読

    Kawamura T, Higashida K, Muraoka I

    Oxidative Medicine and Cellular Longevity   2020年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Induction of Autophagy and Changes in Cellular Metabolism in Glucose Starved C2C12 Myotubes 査読

    Naoya Nakai, Saki Kitai, Noriko Iida, Sachika Inoue, Ken Nakata, Taro Murakami, Kazuhiko Higashida

    J Nutr Sci Vitaminol (Tokyo) .   66 ( 1 )   41 - 47   2020年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   参加形態:共同(副担当)  

  • Application of Molecular Hydrogen as a Novel Antioxidant in Sports Science. 国際誌

    Takuji Kawamura, Kazuhiko Higashida, Isao Muraoka

    Oxidative medicine and cellular longevity   2020   2328768 - 2328768   2020年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Molecular hydrogen (H2) is a colorless, tasteless, odorless, and minimal molecule with high flammability. Although H2 has been thought to be an inert gas in living bodies for many years, an animal study reported that inhalation of H2 gas decreased oxidative stress and suppressed brain injury caused by ischemia and reperfusion injury due to its antioxidant action. Since then, the antioxidant action of H2 has attracted considerable attention and many studies have reported on its benefits. Most studies have reported the effects of H2 on diseases such as cancer, diabetes, cerebral infarction, and Alzheimer's disease. However, little is known regarding its effects on healthy subjects and exercise. Thus far, including our study, only 6 studies have explored the effect of H2 on exercise. H2 is the smallest molecule and therefore can easily penetrate the cellular membrane and rapidly diffuse into organelles. H2 is thought to be able to selectively reduce hydroxyl radicals and peroxynitrite and does not affect physiologically reactive species. H2 can be supplied to the body through multiple routes of administration, such as oral intake of H2 water and H2 bathing. Therefore, H2 may be a potential alternative strategy for conventional exogenous antioxidant interventions in sports science. The purpose of this review is to provide evidence regarding the effects of H2 intake on changes in physiological and biochemical parameters, centering on exercise-induced oxidative stress, for each intake method. Furthermore, this review highlights possible future directions in this area of research.

    DOI: 10.1155/2020/2328768

    PubMed

  • Ketogenic diet feeding improves aerobic metabolism property in extensor digitorum longus muscle of sedentary male rats. 国際誌

    Yuji Ogura, Chiaki Kakehashi, Toshinori Yoshihara, Mitsutoshi Kurosaka, Ryo Kakigi, Kazuhiko Higashida, Sei-Etsu Fujiwara, Tatsuo Akema, Toshiya Funabashi

    PloS one   15 ( 10 )   e0241382   2020年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Recent studies of the ketogenic diet, an extremely high-fat diet with extremely low carbohydrates, suggest that it changes the energy metabolism properties of skeletal muscle. However, ketogenic diet effects on muscle metabolic characteristics are diverse and sometimes countervailing. Furthermore, ketogenic diet effects on skeletal muscle performance are unknown. After male Wistar rats (8 weeks of age) were assigned randomly to a control group (CON) and a ketogenic diet group (KD), they were fed for 4 weeks respectively with a control diet (10% fat, 10% protein, 80% carbohydrate) and a ketogenic diet (90% fat, 10% protein, 0% carbohydrate). After the 4-week feeding period, the extensor digitorum longus (EDL) muscle was evaluated ex vivo for twitch force, tetanic force, and fatigue. We also analyzed the myosin heavy chain composition, protein expression of metabolic enzymes and regulatory factors, and citrate synthase activity. No significant difference was found between CON and KD in twitch or tetanic forces or muscle fatigue. However, the KD citrate synthase activity and the protein expression of Sema3A, citrate synthase, succinate dehydrogenase, cytochrome c oxidase subunit 4, and 3-hydroxyacyl-CoA dehydrogenase were significantly higher than those of CON. Moreover, a myosin heavy chain shift occurred from type IIb to IIx in KD. These results demonstrated that the 4-week ketogenic diet improves skeletal muscle aerobic capacity without obstructing muscle contractile function in sedentary male rats and suggest involvement of Sema3A in the myosin heavy chain shift of EDL muscle.

    DOI: 10.1371/journal.pone.0241382

    PubMed

  • Induction of Autophagy and Changes in Cellular Metabolism in Glucose Starved C2C12 Myotubes.

    Naoya Nakai, Saki Kitai, Noriko Iida, Sachika Inoue, Ken Nakata, Taro Murakami, Kazuhiko Higashida

    Journal of nutritional science and vitaminology   66 ( 1 )   41 - 47   2020年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Mouse myoblast C2C12 cells are commonly used as a model system for investigating the metabolic regulation of skeletal muscle. As it is therefore important to understand the metabolic features of C2C12 cells, we examined the effect of glucose starvation on autophagy in C2C12 myotubes. After culture of C2C12 myotubes with high (HG, 25.0 mM) or low (LG, 5.6 mM) glucose concentrations, the concentration of glucose in the LG group had decreased to 0 mM after 24 h of culture and was around 17 mM after 48 h of culture in the HG group. The concentration of lactate increased from 0 to approximately 9 mM at 24 h and then dropped slightly in the LG group, while it increased linearly to 21 mM in the HG group at 48 h. The phosphorylation of p70 S6 kinase, marker for the protein translation initiation was significantly lower and the ratio of LC3-II/LC3-I, marker for the induction of autophagy was significantly higher in the LG group. GLUT1 and hexokinase II expression were significantly higher in the LG group. Together, these changes in glucose and lactate concentrations in the culture media suggest that C2C12 myotubes depend on anaerobic glycolysis. Our findings also suggest that glucose depletion stimulates the expression of key molecules involved in glycolysis and that cellular autophagy is also activated in C2C12 myotubes.

    DOI: 10.3177/jnsv.66.41

    PubMed

  • A Lactate-based compound containing caffeine in addition to voluntary running exercise decreases subcutaneous fat mass and improves glucose metabolism in obese rats 査読

    56   84 - 91   2019年3月

  • Low-carbohydrate high-protein diet diminishes the insulin response to glucose load via suppression of SGLT-1 in mice. 国際誌

    Kazuhiko Higashida, Shin Terada, Xi Li, Sachika Inoue, Noriko Iida, Saki Kitai, Naoya Nakai

    Bioscience, biotechnology, and biochemistry   83 ( 2 )   365 - 371   2019年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    The purpose of this study was to examine the effects of a low-carbohydrate high-protein (LCHP) diet on the expression of glucose transporters and their relationships to glucose metabolism. Male C57BL/6 mice were fed a normal control or LCHP diet for 2 weeks. An oral glucose tolerance test and insulin tolerance test (ITT) were performed, and the expression of glucose transporters was determined in the gastrocnemius muscle, jejunum and pancreas. The increase in plasma insulin concentrations after glucose administration was reduced in the LCHP group. However, LCHP diet had no effects on peripheral insulin sensitivity or glucose transporters expression in the gastrocnemius and pancreas. Soluble glucose transporter (SGLT)-1 protein content in jejunum was lower in the LCHP group. Taken together, these results suggest that the blunted insulin response after glucose administration in LCHP diet-fed mice might be due to decreased SGLT-1 expression, but not to an increase in peripheral insulin sensitivity. Abbreviations: LCHP: low-carbohydrate high-protein; ITT: insulin tolerance test; GLUT: glucose transporter; SGLT: soluble glucose transporter; OGTT: oral glucose tolerance test; AUC: area under the curve.

    DOI: 10.1080/09168451.2018.1533803

    PubMed

  • Low-carbohydrate high-protein diet diminishes the insulin response to glucose load via suppression of SGLT-1 in mice 査読

    Higashida K, Terada S, Li X, Inoue S, Iida N, Kitai S, Nakai N

    Biosci Biotechnol Biochem   2018年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Time Course of Decrease in Skeletal Muscle Mitochondrial Biogenesis after Discontinuation of High-Fat Diet.

    Li X, Higashida K, Kawamura T, Higuchi M

    J Nutr Sci Vitaminol (Tokyo)   2018年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3177/jnsv.64.233.

  • Effects of exhaustive exercises, with different intensities, on oxidative stress markers in rat plasma and skeletal muscle 査読

    Kawamura T., Fujii R., Li X., Higashida K., Muraoka I.

    Science and Sports   33 ( 3 )   169 - 175   2018年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Science and Sports  

    DOI: 10.1016/j.scispo.2017.08.008

    Scopus

    その他リンク: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85047430962&origin=inward

  • Effects of exhaustive exercises, with different intensities, on oxidative stress markers in rat plasma and skeletal muscle 査読

    Kawamura T, Fujii R, Li X, Higashida K, Muraoka I

    Science and Sports   33 ( 3 )   169 - 175   2018年6月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Elsevier Masson SAS  

    Objectives: The purpose of this study was to examine the effects of exhaustive exercises, with different intensities, on oxidative stress markers in rat plasma and skeletal muscle. Methods: Male Sprague-Dawley rats (n = 24) were randomly divided into the following four groups: control (CON), low-intensity exercise (LE), high-intensity interval exercise (HE) and incremental exercise (IE). The animals in the latter three groups performed exercises in which they ran until exhaustion as follows: Animals in the LE group ran at 20 m/min (6° graded)
    the HE group ran at 40 m/min for 30 s (0° graded) with a rest interval of 60 s between each run
    and the IE group ran at 15 m/min (6° graded), initially, with the running speed gradually increased by 5 m/min, every 10 min. Immediately following the exercise, a blood sample was drawn, and followed by the gastrocnemius muscle and liver were quickly removed. These samples were analysed for lactate concentration, oxidative stress markers, and glycogen contents. Results: Plasma oxidative stress markers in the LE and HE groups demonstrated no change, while plasma protein carbonyl (PC) levels and total antioxidant capacity (TAC) in the IE group were significantly increased compared with the CON group (P &lt
    0.05). In contrast, all the oxidative stress markers, in the skeletal muscle of the three exercise groups, demonstrated no change compared with the CON group. Conclusion: Incremental exhaustive exercise elevates the plasma PC levels and TAC, but does not change skeletal muscle oxidative stress markers in rats.

    DOI: 10.1016/j.scispo.2017.08.008

    Scopus

  • Leucine supplementation after mechanical stimulation activates protein synthesis via L-type amino acid transporter 1 in vitro 査読 国際誌

    Nakai N, Kawano F, Murakami T, Nakata K, Higashida K.

    J Cell Biochem   119 ( 2 )   2094 - 2101   2018年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   参加形態:共同(副担当)  

  • Leucine supplementation after mechanical stimulation activates protein synthesis via L-type amino acid transporter 1 in vitro. 国際誌

    Naoya Nakai, Fuminori Kawano, Taro Murakami, Ken Nakata, Kazuhiko Higashida

    Journal of cellular biochemistry   119 ( 2 )   2094 - 2101   2018年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Branched-chain amino acid supplements consumed following exercise are widely used to increase muscle mass. Although both exercise (ie, mechanical stimulation) and branched-chain amino acid leucine supplementation have been reported to stimulate muscle protein synthesis by activating the mammalian target of rapamycin (mTOR) signaling pathway independently, the mechanisms underlying their synergistic effects are largely unknown. Utilizing cultured differentiated C2C12 myotubes, we established a combination treatment model in which the cells were subjected to cyclic uniaxial mechanical stretching (4 h, 15%, 1 Hz) followed by stimulation with 2 mM leucine for 45 min. Phosphorylation of p70 S6 kinase (p70S6K), an mTOR-regulated marker of protein translation initiation, was significantly increased following mechanical stretching alone but returned to the baseline after 4 h. Leucine supplementation further increased p70S6K phosphorylation, with a greater increase observed in the stretched cells than in the non-stretched cells. Notably, the expression of L-type amino acid transporter 1 (LAT1), a stimulator of the mTOR pathway, was also increased by mechanical stretching, and siRNA-mediated knockdown partially attenuated leucine-induced p70S6K phosphorylation. These results suggest that mechanical stretching promotes LAT1 expression and, consequently, amino acid uptake, leading to enhanced leucine-induced activation of protein synthesis. LAT1 has been demonstrated to be a point of crosstalk between exercise- and nutrition-induced skeletal muscle growth.

    DOI: 10.1002/jcb.26371

    PubMed

  • Time Course of Decrease in Skeletal Muscle Mitochondrial Biogenesis after Discontinuation of High-Fat Diet.

    Xi Li, Kazuhiko Higashida, Takuji Kawamura, Mitsuru Higuchi

    Journal of nutritional science and vitaminology   64 ( 3 )   233 - 238   2018年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    It is known that a high-fat diet induces an increase in mitochondrial biogenesis in skeletal muscle. To examine the time course of decrease in mitochondrial biogenesis in skeletal muscle after discontinuing a high-fat diet feeding, C57BL/6 mice were fed a high-fat diet for 4 wk and then switched to the control diet for another 3 or 7 d. During the high-fat diet withdrawal period, the protein content of the mitochondrial respiratory chain decreased faster than the fatty acid oxidation enzymes. The mitochondrial DNA copy number remained high for at least 1 wk after withdrawing the high-fat diet. These results suggested that after switching to the control diet following a period of high-fat diet, the increased mitochondrial biogenesis levels are maintained for a few days, and the rate of decline is divergent between the different mitochondrial components.

    DOI: 10.3177/jnsv.64.233

    PubMed

  • A Mechanism Underlying Preventive Effect of High-Intensity Training on Colon Cancer. 査読 国際誌

    Kaori Matsuo, Koji Sato, Ken Suemoto, Eri Miyamoto-Mikami, Noriyuki Fuku, Kazuhiko Higashida, Katsunori Tsuji, Yuzhong Xu, Xin Liu, Motoyuki Iemitsu, Takafumi Hamaoka, Izumi Tabata

    Medicine and science in sports and exercise   49 ( 9 )   1805 - 1816   2017年9月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

    INTRODUCTION: We examined effects of high-intensity training on chemically induced aberrant crypt foci (ACF) in rat colon. We also investigated mechanisms that may underlie the results obtained, with a focus on secreted protein acidic and rich in cysteine (SPARC), which has been proposed as an exercise-related factor of colon cancer prevention. METHODS: After an administration of 1,2-dimethylhydrazine, F344 rats executed high-intensity intermittent swimming training (HIIST) (twelve 20-s swimming with a weight [16% body weight] with 10-s pauses between the bouts) 5 d·wk for 4 wk. The acute and chronic effects of the HIIST on SPARC were evaluated in rats. We evaluated the in vitro and in vivo effects of 5' AMP-activated protein kinase (AMPK) activator on SPARC in rat serum and epitrochlearis muscle. In human subjects, we determined serum SPARC after exhaustive bicycling consisting of six to seven bouts of exercise at 170% V˙O2max with 10-s rests between the bouts (high-intensity intermittent bicycling [HIIB]). The SPARC mRNA in human vastus lateralis was measured before and after the HIIB for 4 d·wk for 6 wk (HIIB-training [HIIBT]). RESULTS: The numbers of ACF were lower in the HIIST (47 ± 22) compared with the control (122 ± 47) rats (P < 0.05). SPARC in epitrochlearis and serum after HIIS of the trained rat was higher than that in the control resting rats. In vitro and vivo AMPK stimulation increased mRNA and SPARC protein in rat epitrochlearis, respectively. The human serum SPARC after the HIIB was elevated. SPARC mRNA in human muscle was elevated after the HIIBT. CONCLUSIONS: The results demonstrated that HIIST inhibits 1,2-dimethylhydrazine-induced colon ACF development. This effect may be explained by SPARC induction by the exercise intensity-related factor AMPK, potentially explaining the preventive effects of high-intensity intermittent exercise training against colon cancer.

    DOI: 10.1249/MSS.0000000000001312

    Web of Science

    PubMed

  • PPARβ Is Essential for Maintaining Normal Levels of PGC-1α and Mitochondria and for the Increase in Muscle Mitochondria Induced by Exercise. 査読 国際誌

    Koh JH, Hancock CR, Terada S, Higashida K, Holloszy JO, Han DH

    Cell metabolism   25 ( 5 )   1176 - 1185   2017年5月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.cmet.2017.04.029

    Web of Science

    PubMed

  • Effects of rapid or slow body weight reduction on intramuscular protein degradation pathways during equivalent weight loss on rats 査読

    Nonaka Y., Urashima S., Inai M., Nishimura S., Higashida K., Terada S.

    Physiological Research   66 ( 5 )   823 - 831   2017年1月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)   出版者・発行元:Physiological Research  

    Scopus

    その他リンク: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85037038027&origin=inward

  • A Mechanism Underlying Preventive Effect of High-Intensity Training on Colon Cancer

    Matsuo K, Sato K, Suemoto K, Miyamoto-Mikami E, Fuku N, Higashida K, Tsuji K, Xu Y, Liu X, Iemitsu M, Hamaoka T, Tabata I

    Med Sci Sports Exerc   2017年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1249/MSS.0000000000001312

  • PPARβ Is Essential for Maintaining Normal Levels of PGC-1α and Mitochondria and for the Increase in Muscle Mitochondria Induced by Exercise

    Koh JH, Hancock CR, Terada S, Higashida K, Holloszy JO, Han DH

    Cell Metab   25 ( 5 )   1176 - 1185   2017年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.cmet.2017.04.029

  • Alternate-Day High-Fat Diet Induces an Increase in Mitochondrial Enzyme Activities and Protein Content in Rat Skeletal Muscle. 国際誌

    Xi Li, Kazuhiko Higashida, Takuji Kawamura, Mitsuru Higuchi

    Nutrients   8 ( 4 )   203 - 203   2016年4月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Long-term high-fat diet increases muscle mitochondrial enzyme activity and endurance performance. However, excessive calorie intake causes intra-abdominal fat accumulation and metabolic syndrome. The purpose of this study was to investigate the effect of an alternating day high-fat diet on muscle mitochondrial enzyme activities, protein content, and intra-abdominal fat mass in rats. Male Wistar rats were given a standard chow diet (CON), high-fat diet (HFD), or alternate-day high-fat diet (ALT) for 4 weeks. Rats in the ALT group were fed a high-fat diet and standard chow every other day for 4 weeks. After the dietary intervention, mitochondrial enzyme activities and protein content in skeletal muscle were measured. Although body weight did not differ among groups, the epididymal fat mass in the HFD group was higher than those of the CON and ALT groups. Citrate synthase and beta-hydroxyacyl CoA dehydrogenase activities in the plantaris muscle of rats in HFD and ALT were significantly higher than that in CON rats, whereas there was no difference between HFD and ALT groups. No significant difference was observed in muscle glycogen concentration or glucose transporter-4 protein content among the three groups. These results suggest that an alternate-day high-fat diet induces increases in mitochondrial enzyme activities and protein content in rat skeletal muscle without intra-abdominal fat accumulation.

    DOI: 10.3390/nu8040203

    PubMed

  • 酪酸摂取がラット骨格筋における糖輸送体GLUT-4タンパク質の発現量に及ぼす影響

    野中 雄大, 東田 一彦, 木村 典代, 寺田 新

    体力科学   65 ( 1 )   169 - 176   2016年2月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)   出版者・発行元:(一社)日本体力医学会  

    酪酸摂取がラット骨格筋における糖輸送体GLUT-4タンパク質の発現量に及ぼす影響について検討した。Sprague-Dawley系雄ラットを使用し、予備飼育期間と同じ粉末飼料を摂取させる対照群(C群)、もしくは酪酸を配合した粉末飼料を摂取させる群(SB群)に分けた。飼育期間中の総摂取量、飼料効率は両群間で有意差は認めなかった。クエン酸合成酵素タンパク質発現量は、滑車上筋およびヒラメ筋いずれの筋においても2群間に有意差は認めなかった。ヒラメ筋のアミノレブリン酸合成酵素タンパク質発現量は、C群と比較してSB群で有意に高値を示した。GLUT-4タンパク質発現量は、滑車上筋ではC群と比較してSB群で有意に低値、ヒラメ筋ではC群と比較してSB群で有意に低値を示した。SB群の滑車上筋およびヒラメ筋におけるMCT1タンパク質発現量は、C群に比べて有意に高値を示した。インスリン投与前、即ち空腹時の血糖値には両群間に有意差は認めなかった。食餌条件と時間における交互作用は認めなかったが、食餌条件による主効果を認め、C群と比較してSB群の血糖値は有意に高値を示した。

    DOI: 10.7600/jspfsm.65.169

  • 【未来の扉をひらく運動の研究】 運動による抗糖尿病効果とエピジェネティクス

    寺田 新, 野中 雄大, 東田 一彦

    体育の科学   66 ( 1 )   36 - 43   2016年1月

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    記述言語:日本語   出版者・発行元:(株)杏林書院  

  • 酪酸摂取がラット骨格筋における糖輸送体GLUT-4タンパク質の発現量に及ぼす影響

    野中雄大, 東田一彦, 木村典代, 寺田 新

    体力科学   65 ( 1 )   169 - 176   2016年

     詳細を見る

    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  • Alternate-Day High-Fat Diet Induces an Increase in Mitochondrial Enzyme Activities and Protein Content in Rat Skeletal Muscle

    Li X, Higashida K, Kawamura T, Higuchi M

    Nutrients   8 ( 4 )   E203   2016年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    DOI: 10.3390/nu8040203

  • Dehydroepiandrosterone activates AMP kinase and regulates GLUT4 and PGC-1α expression in C2C12 myotubes. 査読

    Yokokawa T, Sato K, Iwanaka N, Honda H, Higashida K, Iemitsu M, Hayashi T, Hashimoto T

    Biochemical and biophysical research communications   463 ( 1-2 )   42 - 47   2015年7月

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    掲載種別:研究論文(学術雑誌)  

    DOI: 10.1016/j.bbrc.2015.05.013

    Scopus

    PubMed

  • Mixed lactate and caffeine compound increases satellite cell activity and anabolic signals for muscle hypertrophy. 査読 国際誌

    Yoshimi Oishi, Hayato Tsukamoto, Takumi Yokokawa, Keisuke Hirotsu, Mariko Shimazu, Kenji Uchida, Hironori Tomi, Kazuhiko Higashida, Nobumasa Iwanaka, Takeshi Hashimoto

    Journal of applied physiology (Bethesda, Md. : 1985)   118 ( 6 )   742 - 9   2015年3月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    We examined whether a mixed lactate and caffeine compound (LC) could effectively elicit proliferation and differentiation of satellite cells or activate anabolic signals in skeletal muscles. We cultured C2C12 cells with either lactate or LC for 6 h. We found that lactate significantly increased myogenin and follistatin protein levels and phosphorylation of P70S6K while decreasing the levels of myostatin relative to the control. LC significantly increased protein levels of Pax7, MyoD, and Ki67 in addition to myogenin, relative to control. LC also significantly increased follistatin expression relative to control and stimulated phosphorylation of mTOR and P70S6K. In an in vivo study, male F344/DuCrlCrlj rats were assigned to control (Sed, n = 10), exercise (Ex, n = 12), and LC supplementation (LCEx, n = 13) groups. LC was orally administered daily. The LCEx and Ex groups were exercised on a treadmill, running for 30 min at low intensity every other day for 4 wk. The LCEx group experienced a significant increase in the mass of the gastrocnemius (GA) and tibialis anterior (TA) relative to both the Sed and Ex groups. Furthermore, the LCEx group showed a significant increase in the total DNA content of TA compared with the Sed group. The LCEx group experienced a significant increase in myogenin and follistatin expression of GA relative to the Ex group. These results suggest that administration of LC can effectively increase muscle mass concomitant with elevated numbers of myonuclei, even with low-intensity exercise training, via activated satellite cells and anabolic signals.

    DOI: 10.1152/japplphysiol.00054.2014

    PubMed

  • PGC-1α mediates a rapid, exercise-induced downregulation of glycogenolysis in rat skeletal muscle. 国際誌

    Sang Hyun Kim, Jin Ho Koh, Kazuhiko Higashida, Su Ryun Jung, John O Holloszy, Dong-Ho Han

    The Journal of physiology   593 ( 3 )   635 - 43   2015年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    KEY POINTS: Long-term endurance exercise training results in a reduction in the rates of muscle glycogen depletion and lactic acid accumulation during submaximal exercise; this adaptation is mediated by an increase in muscle mitochondria. There is evidence suggesting that short-term training induces adaptations that downregulate glycogenolysis before there is an increase in functional mitochondria. We discovered that a single long bout of exercise induces decreases in expression of glycogenolytic and glycolytic enzymes in rat skeletal muscle; this adaptation results in slower rates of glycogenolysis and lactic acid accumulation in muscle during contractile activity. Two additional days of training amplified the adaptive response, which appears to be mediated by PGC-1α; this adaptation is biologically significant, because glycogen depletion and lactic acid accumulation are major causes of muscle fatigue. ABSTRACT: Endurance exercise training can increase the ability to perform prolonged strenuous exercise. The major adaptation responsible for this increase in endurance is an increase in muscle mitochondria. This adaptation occurs too slowly to provide a survival advantage when there is a sudden change in environment that necessitates prolonged exercise. In the present study, we discovered another, more rapid adaptation, a downregulation of expression of the glycogenolytic and glycolytic enzymes in muscle that mediates a slowing of muscle glycogen depletion and lactic acid accumulation. This adaptation, which appears to be mediated by PGC-1α, occurs in response to a single exercise bout and is further enhanced by two additional daily exercise bouts. It is biologically significant, because glycogen depletion and lactic acid accumulation are two of the major causes of muscle fatigue and exhaustion.

    DOI: 10.1113/jphysiol.2014.283820

    PubMed

  • 異なる脂質含量の食餌がラットの骨格筋ミトコンドリア酵素活性および持久性運動パフォーマンスに及ぼす影響

    東田一彦, 園生智広, 藤本恵理, 樋口満

    スポーツ科学研究   12   137 - 144   2015年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  • PGC-1α mediates a rapid, exercise-induced downregulation of glycogenolysis in rat skeletal muscle

    Kim SH, Koh JH, Higashida K, Jung SR, Holloszy JO, Han DH

    J Physiol   593 ( 3 )   635 - 643   2015年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Mixed lactate and caffeine compound increases satellite cell activity and anabolic signals for muscle hypertrophy.

    Oishi Y, Tsukamoto H, Yokokawa T, Hirotsu K, Shimazu M, Uchida K, Tomi H, Higashida K, Iwanaka N, Hashimoto T

    J Appl Physiol   118 ( 6 )   742 - 749   2015年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Dehydroepiandrosterone activates AMP kinase and regulates GLUT4 and PGC-1α expression in C2C12 myotubes

    Yokokawa T, Sato K, Iwanaka N, Honda H, Higashida K, Iemitsu M, Hayashi T, Hashimoto T

    Biochem Biophys Res Commun   463   42 - 47   2015年

     詳細を見る

    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Corticosterone accelerates atherosclerosis in the apolipoprotein E-deficient mouse. 国際誌

    Mitsuharu Okutsu, Vitor A Lira, Kazuhiko Higashida, Jonathan Peake, Mitsuru Higuchi, Katsuhiko Suzuki

    Atherosclerosis   232 ( 2 )   414 - 9   2014年2月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

    Chronic stress is an important risk factor for atherosclerosis, which is a chief process in the development of cardiovascular disease. Increased circulating levels of corticosterone have been documented in several animal models of chronic stress. However, it remains to be established whether corticosterone is sufficient to exacerbate atherosclerosis. To test this hypothesis, apolipoprotein E (ApoE)-deficient mice were fed a high-fat diet for 13 weeks with exposure to either corticosterone or vehicle in the drinking water (CORT and Con). Corticosterone treatment significantly increased atherosclerotic plaque area at the aortic root. Such exacerbation of atherosclerosis was accompanied by significantly lower levels of circulating white blood cells and serum interleukin-1β (IL-1β), and significantly elevated serum concentrations of total cholesterol, low-density lipoprotein (LDL), very-low-density lipoprotein (VLDL) and small dense low-density lipoprotein (sd-LDL) in CORT mice when compared to Con mice. These findings demonstrate that corticosterone is sufficient to exacerbate atherosclerosis in vivo despite its anti-inflammatory properties and that this marked pro-atherogenic phenotype is primarily associated with increased dyslipidaemia.

    DOI: 10.1016/j.atherosclerosis.2013.11.076

    PubMed

  • Corticosterone accelerates atherosclerosis in the apolipoprotein E-deficient mouse

    Okutsu M, Lira VA, Higashida K, Peake J, Higuchi M, Suzuki K

    Atherosclerosis   232 ( 2 )   414 - 419   2014年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • 【運動刺激の正体を探る】 運動刺激に伴う骨格筋代謝機能の適応

    寺田 新, 東田 一彦

    体育の科学   63 ( 8 )   608 - 615   2013年8月

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    記述言語:日本語   出版者・発行元:(株)杏林書院  

  • β-Adrenergic Stimulation Does Not Activate p38 MAPKinase or Induce PGC-1α in Skeletal Muscle

    Kim SH, Asaka M, Higashida K, Takahashi Y, Holloszy JO, Han DH

    Am J Physiol Endocrinol Metab   304 ( 8 )   844 - 852   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • 間欠的絶食による摂取エネルギー制限がラット骨格筋のPGC-1αおよびGLUT-4 蛋白発現量に及ぼす影響

    東田 一彦、樋口 満、寺田 新

    スポーツ科学研究   10   1 - 11   2013年

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)  

  • Effects of alternate-day fasting on high-fat diet-induced insulin resistance in rat skeletal muscle

    Higashida K, Fujimoto E, Higuchi M, Terada S

    Life Sci   93 ( 5 )   208 - 213   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Effects of Resveratrol and SIRT1 on PGC-1α Activity and Mitochondrial Biogenesis: A Reevaluation

    Higashida K, Kim SH, Jung SR, Asaka M, Holloszy JO, Han DH

    PLOS Biology   11 ( 7 )   e1001603   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Regulation of Skeletal muscle GLUT-4 expression by exercise and nutritional stimuli

    Higashida K, Tabata I, Higuchi M, Terada S

    J Phys Fitness Sports Med   2 ( 3 )   355 - 360   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Ursolic acid stimulates mTORC1 signaling after resistance exercise in rat skeletal muscle

    Ogasawara R, Sato K, Higashida K, Nakazato K, Fujita S

    Am J Physiol Endocrinol Metab   305 ( 6 )   760 - 765   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Modest hypoxia significantly reduces triglyceride content and lipid droplet size in 3T3-L1 adipocytes

    Hashimoto T, Yokokawa T, Endo Y, Iwanaka N, Higashida K, Taguchi S

    Biochem Biophys Res Commun   440 ( 1 )   43 - 49   2013年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • 04生-30-ポ-59 一過性の持久的運動が骨格筋脂肪滴関連タンパク質の発現量に及ぼす影響(04 運動生理学,一般研究発表抄録)

    東田 一彦, 樋口 満, 橋本 健志

    日本体育学会大会予稿集   64   182 - 183   2013年

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    記述言語:日本語   出版者・発行元:社団法人日本体育学会  

    DOI: 10.20693/jspehss.64.182_3

    CiNii Books

  • The AMPK beta2 subunit is required for energy homeostasis during metabolic stress

    Dasgupta B, Ju JS, Sasaki Y, Liu X, Jung SR, Higashida K, Lindquist D, Milbrandt J

    Mol Cell Biol   32 ( 14 )   2837 - 2848   2012年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Ginsenoside Re rapidly reverses insulin resistance in muscles of high-fat diet fed rats

    Han DH, Kim SH, Higashida K, Jung SR, Polonsky KS, Klein S, Holloszy JO

    Metabolism   61 ( 11 )   1615 - 1621   2011年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Does calorie restriction induce mitochondrial biogenesis? A reevaluation

    Hancock CR, Han DH, Higashida K, Kim SH, Holloszy JO

    FASEB J   25 ( 2 )   785 - 791   2011年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Deficiency of the mitochondrial electron transport chain in muscle does not cause insulin resistance

    Han DH, Hancock CR, Jung SR, Higashida K, Kim SH, Holloszy JO

    PLoS One   6 ( 5 )   e19739   2011年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Normal Adaptations to Exercise Despite Protection Against Oxidative Stress

    Higashida K, Kim SH, Higuchi M, Holloszy JO, Han DH

    Am J Physiol Endocrinol Metab   301 ( 5 )   779 - 784   2011年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Dissociation between PGC-1alpha and GLUT-4 expression in skeletal muscle of rats fed a high-fat diet

    Higashida K, Higuchi M, Terada S

    J Nutr Sci Vitaminol (Tokyo)   55 ( 6 )   486 - 491   2009年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Prolonged exercise training induces long-term enhancement of HSP70 expression in rat plantaris muscle

    Ogata T, Oishi Y, Higashida K, Higuchi M, Muraoka I

    Am J Physiol Regul Integr Comp Physiol   296 ( 5 )   1557 - 1563   2009年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

  • Potential role of lipin-1 in exercise-induced mitochondrial biogenesis

    Higashida K, Higuchi M, Terada S

    Biochem Biophys Res Commun   374 ( 3 )   587 - 591   2008年

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    記述言語:英語   掲載種別:研究論文(学術雑誌)  

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MISC

  • 筋収縮によるmTORC1活性化はPGC-1α発現量の増加に関与しない

    小笠原 理紀, 北岡 祐, 東田 一彦, 中里 浩一, 藤田 聡

    体力科学   64 ( 6 )   568 - 568   2015年12月

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    記述言語:日本語   出版者・発行元:(一社)日本体力医学会  

  • 筋収縮によるmTORC1活性化はPGC-1α発現量の増加に関与しない

    小笠原 理紀, 北岡 祐, 東田 一彦, 中里 浩一, 藤田 聡

    体力科学   64 ( 6 )   568 - 568   2015年12月

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    記述言語:日本語   出版者・発行元:(一社)日本体力医学会  

  • Alternate-day High-fat Diet Feeding Induces Insulin resistance Despite No Abdominal Fat Accumulation And Mitochondrial Dysfunction

    Kazuhiko Higashida, Takuji Kawamura, Isao Muraoka, Mitsuru Higuchi

    MEDICINE AND SCIENCE IN SPORTS AND EXERCISE   47 ( 5 )   411 - 412   2015年5月

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    記述言語:英語   掲載種別:研究発表ペーパー・要旨(国際会議)   出版者・発行元:LIPPINCOTT WILLIAMS & WILKINS  

    DOI: 10.1249/01.mss.0000477554.44047.b8

    Web of Science

  • 異なる脂質含量の食餌がラットの骨格筋ミトコンドリア酵素活性および持久性運動パフォーマンスに及ぼす影響

    東田 一彦, 園生 智広, 藤本 恵理, 樋口 満

    スポーツ科学研究   12   137 - 144   2015年

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    記述言語:日本語   出版者・発行元:早稲田大学スポーツ科学学術院  

    CiNii Books

  • L6細胞に対するカフェイン曝露がSIRT3発現に与える影響

    横川拓海, 岩中伸壮, 東田一彦, 橋本健志

    体力科学   62 ( 6 )   2013年

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  • 間欠的絶食による摂取エネルギー制限がラット骨格筋のPGC-1αおよびGLUT-4蛋白発現量に及ぼす影響

    東田 一彦, 樋口 満, 寺田 新

    スポーツ科学研究   10   1 - 11   2013年

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    記述言語:日本語   出版者・発行元:早稲田大学スポーツ科学学術院  

    CiNii Books

    その他リンク: http://search.jamas.or.jp/link/ui/2015118621

  • 持久性運動機能に及ぼす抗酸化ビタミン,ポリフェノール摂取の影響 (特集 スポーツ栄養の最近の動向) -- (基礎編)

    東田 一彦, 樋口 満

    臨床スポーツ医学   29 ( 9 )   881 - 885   2012年9月

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    記述言語:日本語   出版者・発行元:文光堂  

    CiNii Books

    その他リンク: http://search.jamas.or.jp/link/ui/2012322488

  • Normal adaptations to exercise despite protection against oxidative stress

    Kazuhiko Higashida, Sang Hyun Kim, Mitsuru Higuchi, John O. Holloszy, Dong-Ho Han

    AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM   301 ( 5 )   E779 - E784   2011年11月

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    記述言語:英語   出版者・発行元:AMER PHYSIOLOGICAL SOC  

    Higashida K, Kim SH, Higuchi M, Holloszy JO, Han DH. Normal adaptations to exercise despite protection against oxidative stress. Am J Physiol Endocrinol Metab 301: E779-E784, 2011. First published July 12, 2011; doi: 10.1152/ajpendo.00655.2010.-It has been reported that supplementation with the antioxidant vitamins C and E prevents the adaptive increases in mitochondrial biogenesis and GLUT4 expression induced by endurance exercise. We reevaluated the effects of these antioxidants on the adaptive responses of rat skeletal muscle to swimming in a short-term study consisting of 9 days of vitamins C and E with exercise during the last 3 days and a longer-term study consisting of 8 wk of antioxidant vitamins with exercise during the last 3 wk. The rats in the antioxidant groups were given 750 mg . kg body wt(-1) . day(-1) vitamin C and 150 mg . kg body wt(-1) . day(-1) vitamin E. In rats euthanized immediately after exercise, plasma TBARs were elevated in the control rats but not in the antioxidant-supplemented rats, providing evidence for an antioxidant effect. In rats euthanized 18 h after exercise there were large increases in insulin responsiveness of glucose transport in epitrochlearis muscles mediated by an approximately twofold increase in GLUT4 expression in both the short-and long-term treatment groups. The protein levels of a number of mitochondrial marker enzymes were also increased about twofold. Superoxide dismutases (SOD) 1 and 2 were increased about twofold in triceps muscle after 3 days of exercise, but only SOD2 was increased after 3 wk of exercise. There were no differences in the magnitudes of any of these adaptive responses between the control and antioxidant groups. These results show that very large doses of antioxidant vitamins do not prevent the exercise-induced adaptive responses of muscle mitochondria, GLUT4, and insulin action to exercise and have no effect on the level of these proteins in sedentary rats.

    DOI: 10.1152/ajpendo.00655.2010

    Web of Science

  • Normal adaptations to exercise despite protection against oxidative stress. 国際誌

    Kazuhiko Higashida, Sang Hyun Kim, Mitsuru Higuchi, John O Holloszy, Dong-Ho Han

    American journal of physiology. Endocrinology and metabolism   301 ( 5 )   E779-84 - E784   2011年11月

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    記述言語:英語   出版者・発行元:AMER PHYSIOLOGICAL SOC  

    It has been reported that supplementation with the antioxidant vitamins C and E prevents the adaptive increases in mitochondrial biogenesis and GLUT4 expression induced by endurance exercise. We reevaluated the effects of these antioxidants on the adaptive responses of rat skeletal muscle to swimming in a short-term study consisting of 9 days of vitamins C and E with exercise during the last 3 days and a longer-term study consisting of 8 wk of antioxidant vitamins with exercise during the last 3 wk. The rats in the antioxidant groups were given 750 mg·kg body wt(-1)·day(-1) vitamin C and 150 mg·kg body wt(-1)·day(-1) vitamin E. In rats euthanized immediately after exercise, plasma TBARs were elevated in the control rats but not in the antioxidant-supplemented rats, providing evidence for an antioxidant effect. In rats euthanized 18 h after exercise there were large increases in insulin responsiveness of glucose transport in epitrochlearis muscles mediated by an approximately twofold increase in GLUT4 expression in both the short- and long-term treatment groups. The protein levels of a number of mitochondrial marker enzymes were also increased about twofold. Superoxide dismutases (SOD) 1 and 2 were increased about twofold in triceps muscle after 3 days of exercise, but only SOD2 was increased after 3 wk of exercise. There were no differences in the magnitudes of any of these adaptive responses between the control and antioxidant groups. These results show that very large doses of antioxidant vitamins do not prevent the exercise-induced adaptive responses of muscle mitochondria, GLUT4, and insulin action to exercise and have no effect on the level of these proteins in sedentary rats.

    DOI: 10.1152/ajpendo.00655.2010

    Web of Science

    PubMed

  • Deficiency of the mitochondrial electron transport chain in muscle does not cause insulin resistance. 国際誌

    Dong-Ho Han, Chad R Hancock, Su Ryun Jung, Kazuhiko Higashida, Sang Hyun Kim, John O Holloszy

    PloS one   6 ( 5 )   e19739   2011年5月

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    記述言語:英語   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    BACKGROUND: It has been proposed that muscle insulin resistance in type 2 diabetes is due to a selective decrease in the components of the mitochondrial electron transport chain and results from accumulation of toxic products of incomplete fat oxidation. The purpose of the present study was to test this hypothesis. METHODOLOGY/PRINCIPAL FINDINGS: Rats were made severely iron deficient, by means of an iron-deficient diet. Iron deficiency results in decreases of the iron containing mitochondrial respiratory chain proteins without affecting the enzymes of the fatty acid oxidation pathway. Insulin resistance was induced by feeding iron-deficient and control rats a high fat diet. Skeletal muscle insulin resistance was evaluated by measuring glucose transport activity in soleus muscle strips. Mitochondrial proteins were measured by Western blot. Iron deficiency resulted in a decrease in expression of iron containing proteins of the mitochondrial respiratory chain in muscle. Citrate synthase, a non-iron containing citrate cycle enzyme, and long chain acyl-CoA dehydrogenase (LCAD), used as a marker for the fatty acid oxidation pathway, were unaffected by the iron deficiency. Oleate oxidation by muscle homogenates was increased by high fat feeding and decreased by iron deficiency despite high fat feeding. The high fat diet caused severe insulin resistance of muscle glucose transport. Iron deficiency completely protected against the high fat diet-induced muscle insulin resistance. CONCLUSIONS/SIGNIFICANCE: The results of the study argue against the hypothesis that a deficiency of the electron transport chain (ETC), and imbalance between the ETC and β-oxidation pathways, causes muscle insulin resistance.

    DOI: 10.1371/journal.pone.0019739

    Web of Science

    PubMed

  • Deficiency of the Mitochondrial Electron Transport Chain in Muscle Does Not Cause Insulin Resistance

    Dong-Ho Han, Chad R. Hancock, Su Ryun Jung, Kazuhiko Higashida, Sang Hyun Kim, John O. Holloszy

    PLOS ONE   6 ( 5 )   e19739   2011年5月

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    記述言語:英語   出版者・発行元:PUBLIC LIBRARY SCIENCE  

    Background: It has been proposed that muscle insulin resistance in type 2 diabetes is due to a selective decrease in the components of the mitochondrial electron transport chain and results from accumulation of toxic products of incomplete fat oxidation. The purpose of the present study was to test this hypothesis.
    Methodology/Principal Findings: Rats were made severely iron deficient, by means of an iron-deficient diet. Iron deficiency results in decreases of the iron containing mitochondrial respiratory chain proteins without affecting the enzymes of the fatty acid oxidation pathway. Insulin resistance was induced by feeding iron-deficient and control rats a high fat diet. Skeletal muscle insulin resistance was evaluated by measuring glucose transport activity in soleus muscle strips. Mitochondrial proteins were measured by Western blot. Iron deficiency resulted in a decrease in expression of iron containing proteins of the mitochondrial respiratory chain in muscle. Citrate synthase, a non-iron containing citrate cycle enzyme, and long chain acyl-CoA dehydrogenase (LCAD), used as a marker for the fatty acid oxidation pathway, were unaffected by the iron deficiency. Oleate oxidation by muscle homogenates was increased by high fat feeding and decreased by iron deficiency despite high fat feeding. The high fat diet caused severe insulin resistance of muscle glucose transport. Iron deficiency completely protected against the high fat diet-induced muscle insulin resistance.
    Conclusions/Significance: The results of the study argue against the hypothesis that a deficiency of the electron transport chain (ETC), and imbalance between the ETC and beta-oxidation pathways, causes muscle insulin resistance.

    DOI: 10.1371/journal.pone.0019739

    Web of Science

  • Does calorie restriction induce mitochondrial biogenesis? A reevaluation

    Chad R. Hancock, Dong-Ho Han, Kazuhiko Higashida, Sang Hyun Kim, John O. Holloszy

    FASEB JOURNAL   25 ( 2 )   785 - 791   2011年2月

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    記述言語:英語   出版者・発行元:FEDERATION AMER SOC EXP BIOL  

    It has been reported that 30% calorie restriction (CR) for 3 mo results in large increases in mitochondrial biogenesis in heart, brain, liver, and adipose tissue, with concomitant increases in respiration and ATP synthesis. We found these results surprising, and performed this study to determine whether 30% CR does induce an increase in mitochondria in heart, brain, liver, adipose tissue, and/or skeletal muscle. To this end, we measured the levels of a range of mitochondrial proteins, and mRNAs. With the exception of long-chain acyl-CoA dehydrogenase protein level, which was increased similar to 60% in adipose tissue, none of the mitochondrial proteins or mRNAs that we measured were increased in rats subjected to 30% CR for 14 wk. There was also no increase in citrate synthase activity. Because it is not possible to have an increase in mitochondria without any increase in key mitochondrial proteins, we conclude that 30% CR does not induce an increase in mitochondria in heart, brain, liver, adipose tissue, or skeletal muscle in laboratory rodents.-Hancock, C. R., Han, D.-H., Higashida, K., Kim, S. H., Holloszy, J. O. Does calorie restriction induce mitochondrial biogenesis? A reevaluation. FASEB J. 25, 785-791 (2011). www.fasebj.org

    DOI: 10.1096/fj.10-170415

    Web of Science

  • Does calorie restriction induce mitochondrial biogenesis? A reevaluation. 国際誌

    Chad R Hancock, Dong-Ho Han, Kazuhiko Higashida, Sang Hyun Kim, John O Holloszy

    FASEB journal : official publication of the Federation of American Societies for Experimental Biology   25 ( 2 )   785 - 91   2011年2月

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    記述言語:英語  

    It has been reported that 30% calorie restriction (CR) for 3 mo results in large increases in mitochondrial biogenesis in heart, brain, liver, and adipose tissue, with concomitant increases in respiration and ATP synthesis. We found these results surprising, and performed this study to determine whether 30% CR does induce an increase in mitochondria in heart, brain, liver, adipose tissue, and/or skeletal muscle. To this end, we measured the levels of a range of mitochondrial proteins, and mRNAs. With the exception of long-chain acyl-CoA dehydrogenase protein level, which was increased ∼60% in adipose tissue, none of the mitochondrial proteins or mRNAs that we measured were increased in rats subjected to 30% CR for 14 wk. There was also no increase in citrate synthase activity. Because it is not possible to have an increase in mitochondria without any increase in key mitochondrial proteins, we conclude that 30% CR does not induce an increase in mitochondria in heart, brain, liver, adipose tissue, or skeletal muscle in laboratory rodents.

    DOI: 10.1096/fj.10-170415

    PubMed

  • Dissociation between PGC-1alpha and GLUT-4 expression in skeletal muscle of rats fed a high-fat diet.

    Kazuhiko Higashida, Mitsuru Higuchi, Shin Terada

    Journal of nutritional science and vitaminology   55 ( 6 )   486 - 91   2009年12月

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    記述言語:英語  

    It has recently been reported that a 4-wk high-fat diet gradually increases skeletal muscle peroxisome proliferator activated receptor (PPAR) gamma coactivator-1alpha (PGC-1alpha) protein content, which has been suggested to regulate GLUT-4 gene transcription. However, it has not been reported that a high-fat diet enhances GLUT-4 mRNA expression and protein content in skeletal muscle, suggesting that an increase in PGC-1alpha protein content is not sufficient to induce muscle GLUT-4 biogenesis in a high-fat fed animal. Therefore, we first evaluated the relationship between PGC-1alpha and GLUT-4 expression in skeletal muscle of rats fed a high-fat diet for 4 wk. The PGC-1alpha protein content in rat epitrochlearis muscle significantly increased by twofold after the 4-wk high-fat diet feeding. However, the high-fat diet had no effect on GLUT-4 protein content and induced a 30% decrease in GLUT-4 mRNA expression in rat skeletal muscle (p<0.05). To clarify the mechanism by which a high-fat diet downregulates GLUT-4 mRNA expression, we next examined the effect of PPARdelta activation, which is known to occur in response to a high-fat diet, on GLUT-4 mRNA expression in L6 myotubes. Incubation with 500 nM GW501516 (PPARdelta activator) for 24 h significantly decreased GLUT-4 mRNA in L6 myotubes. Taken together, these findings suggest that a high-fat diet downregulates GLUT-4 mRNA, possibly through the activation of PPARdelta, despite an increase in PGC-1alpha protein content in rat skeletal muscle, and that a posttranscriptional regulatory mechanism maintains GLUT-4 protein content in skeletal muscle of rats fed a high-fat diet.

    DOI: 10.3177/jnsv.55.486

    Scopus

    PubMed

  • Prolonged exercise training induces long-term enhancement of HSP70 expression in rat plantaris muscle

    Tomonori Ogata, Yasuharu Oishi, Kazuhiko Higashida, Mitsuru Higuchi, Isao Muraoka

    AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY   296 ( 5 )   R1557 - R1563   2009年5月

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    記述言語:英語   出版者・発行元:AMER PHYSIOLOGICAL SOC  

    Ogata T, Oishi Y, Higashida K, Higuchi M, Muraoka I. Prolonged exercise training induces long-term enhancement of HSP70 expression in rat plantaris muscle. Am J Physiol Regul Integr Comp Physiol 296: R1557-R1563, 2009. First published February 25, 2009; doi:10.1152/ajpregu.90911.2008.-Skeletal muscle may develop adaptive molecular chaperone enhancements as a potential defense system through repeated daily exercise stimulation. The present study investigated whether prolonged exercise training alters the expression of molecular chaperone proteins for the long term in skeletal muscle. Mature male Wistar rats were subjected for 8 wk to either a single bout of acute intermittent treadmill running (30 m/min, 5 min x 4, 5 degrees grade) or prolonged treadmill running training (15-40 m/min, 5 min x 4, 5-7 degrees grade). Levels of five molecular chaperone proteins [ heat shock protein (HSP) 25, HSP60, glucose-regulated protein (GRP) 78, HSP70, and heat shock cognate (HSC) 70] were measured in response to acute exercise and prolonged training. HSP70 levels were increased 6 and 24 h after acute exercise, but expression returned to control level within 2 days. In contrast, prolonged training had a long-term effect on HSP70 expression. Levels of HSP70 were notably increased by 4.5-fold over control 2 days after prolonged training; the enhancement was maintained for at least 14 days after training ended. However, other molecular chaperone proteins did not show adaptive changes in response to prolonged training. In addition, HSP70 enhancement by prolonged exercise training was not accompanied by transcription of HSP70 mRNA. These findings demonstrate that prolonged training can induce long-term enhancement of HSP70 expression without change at the mRNA level in skeletal muscle.

    DOI: 10.1152/ajpregu.90911.2008

    Web of Science

  • Prolonged exercise training induces long-term enhancement of HSP70 expression in rat plantaris muscle. 国際誌

    Tomonori Ogata, Yasuharu Oishi, Kazuhiko Higashida, Mitsuru Higuchi, Isao Muraoka

    American journal of physiology. Regulatory, integrative and comparative physiology   296 ( 5 )   R1557-63 - R1563   2009年5月

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    記述言語:英語   出版者・発行元:AMER PHYSIOLOGICAL SOC  

    Skeletal muscle may develop adaptive molecular chaperone enhancements as a potential defense system through repeated daily exercise stimulation. The present study investigated whether prolonged exercise training alters the expression of molecular chaperone proteins for the long term in skeletal muscle. Mature male Wistar rats were subjected for 8 wk to either a single bout of acute intermittent treadmill running (30 m/min, 5 min x 4, 5 degrees grade) or prolonged treadmill running training (15-40 m/min, 5 min x 4, 5-7 degrees grade). Levels of five molecular chaperone proteins [heat shock protein (HSP)25, HSP60, glucose-regulated protein (GRP)78, HSP70, and heat shock cognate (HSC)70] were measured in response to acute exercise and prolonged training. HSP70 levels were increased 6 and 24 h after acute exercise, but expression returned to control level within 2 days. In contrast, prolonged training had a long-term effect on HSP70 expression. Levels of HSP70 were notably increased by 4.5-fold over control 2 days after prolonged training; the enhancement was maintained for at least 14 days after training ended. However, other molecular chaperone proteins did not show adaptive changes in response to prolonged training. In addition, HSP70 enhancement by prolonged exercise training was not accompanied by transcription of HSP70 mRNA. These findings demonstrate that prolonged training can induce long-term enhancement of HSP70 expression without change at the mRNA level in skeletal muscle.

    DOI: 10.1152/ajpregu.90911.2008

    Web of Science

    PubMed

  • Dissociation between PGC-1α and GLUT-4 expression in skeletal muscle of rats fed a high-fat diet

    Kazuhiko Higashida, Mitsuru Higuchi, Shin Terada

    Journal of Nutritional Science and Vitaminology   55 ( 6 )   486 - 491   2009年

     詳細を見る

    記述言語:英語  

    It has recently been reported that a 4-wk high-fat diet gradually increases skeletal muscle peroxisome proliferator activated receptor (PPAR) γ coactivator-1 α (PGC-1α) protein content, which has been suggested to regulate GLUT-4 gene transcription. However, it has not been reported that a high-fat diet enhances GLUT-4 mRNA expression and protein content in skeletal muscle, suggesting that an increase in PGC-lα protein content is not sufficient to induce muscle GLUT-4 biogenesis in a high-fat fed animal. Therefore, we first evaluated the relationship between PGC-1α and GLUT-4 expression in skeletal muscle of rats fed a high-fat diet for 4 wk. The PGC-1 protein content in rat epitrochlearis muscle significantly increased by twofold after the 4-wk high-fat diet feeding. However, the high-fat diet had no effect on GLUT-4 protein content and induced a 30% decrease in GLUT-4 mRNA expression in rat skeletal muscle (p&lt
    0.05). To clarify the mechanism by which a high-fat diet downregulates GLUT-4 mRNA expression, we next examined the effect of PPARδ activation, which is known to occur in response to a high-fat diet, on GLUT-4 mRNA expression in L6 myotubes. Incubation with 500 nM GW501516 (PPARδ activator) for 24 h significantly decreased GLUT-4 mRNA in L6 myotubes. Taken together, these findings suggest that a high-fat diet downregulates GLUT-4 mRNA, possibly through the activation of PPARδ, despite an increase in PGC-1α protein content in rat skeletal muscle, and that a posttranscriptional regulatory mechanism maintains GLUT-4 protein content in skeletal muscle of rats fed a high-fat diet.

    DOI: 10.3177/jnsv.55.486

    Scopus

    PubMed

  • 260. 間欠的絶食がラット骨格筋の糖輸送体GLUT-4発現量に及ぼす影響(代謝,一般口演,第63回日本体力医学会大会)

    寺田 新, 東田 一彦, 園生 智広, 樋口 満

    体力科學   57 ( 6 )   762 - 762   2008年12月

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    記述言語:日本語   出版者・発行元:日本体力医学会  

    CiNii Books

  • 272. 低強度長時間水泳運動後のラット骨格筋PGC-1αの変化(代謝,一般口演,第63回日本体力医学会大会)

    藤本 恵理, 寺田 新, 東田 一彦, 樋口 満, 田畑 泉

    体力科學   57 ( 6 )   768 - 768   2008年12月

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    記述言語:日本語   出版者・発行元:日本体力医学会  

    CiNii Books

  • 266. 高脂肪食摂取がラット骨格筋の糖輸送体GLUT-4発現量に及ぼす影響(代謝,一般口演,第63回日本体力医学会大会)

    東田 一彦, 園生 智広, 樋口 満, 寺田 新

    体力科學   57 ( 6 )   765 - 765   2008年12月

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    記述言語:日本語   出版者・発行元:日本体力医学会  

    CiNii Books

  • Potential role of lipin-1 in exercise-induced mitochondrial biogenesis. 国際誌

    Kazuhiko Higashida, Mitsuru Higuchi, Shin Terada

    Biochemical and biophysical research communications   374 ( 3 )   587 - 91   2008年9月

     詳細を見る

    記述言語:英語   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Endurance exercise induces mitochondrial biogenesis in skeletal muscle. It has been shown that lipin-1 acts as a transcriptional coactivator in liver, and stimulates gene expression of mitochondrial enzymes. We hypothesized that lipin-1 might be involved in exercise-induced mitochondrial biogenesis in skeletal muscle. The present investigation first demonstrated that lipin-1 mRNA in rat triceps muscle was increased by approximately 2-fold after an acute bout of endurance swimming exercise. Second, ectopic expression of lipin-1 in L6 myotube increased carnitine palmitoyltransferase-1 and delta-aminolevulinate synthase gene expression. Finally, lipin-1 mRNA expression in rat triceps muscle was significantly elevated at 6h after subcutaneous injections of 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) or clenbuterol, which are 5'-AMP-activated protein kinase (AMPK) and beta2-adrenergic receptor (beta2-AR) activators, respectively. These results may suggest that enhanced expression of lipin-1 is involved in exercise-induced mitochondrial enzyme adaptations, possibly through AMPK- and beta2-AR-related mechanisms.

    DOI: 10.1016/j.bbrc.2008.07.079

    Web of Science

    PubMed

  • Potential role of lipin-1 in exercise-induced mitochondrial biogenesis

    Kazuhiko Higashida, Mitsuru Higuchi, Shin Terada

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   374 ( 3 )   587 - 591   2008年9月

     詳細を見る

    記述言語:英語   出版者・発行元:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Endurance exercise induces mitochondrial biogenesis in skeletal muscle. It has been shown that lipin-1 acts as a transcriptional coactivator in liver, and Stimulates gene expression of mitochondrial enzymes. We hypothesized that lipin-1 might be involved in exercise-induced mitochondrial biogenesis in skeletal muscle. The present investigation first demonstrated that lipin-1 mRNA in rat triceps muscle was increased by approximately 2-fold after an acute bout of endurance swimming exercise. Second, ectopic expression of lipin-1 in L6 myotube increased carnitine palmitoyltransferase-1 and delta-aminolevulinate synthase gene expression. Finally, lipin-1 mRNA expression in rat triceps muscle was significantly elevated at 6 h after subcutaneous injections of 5-aminomidazole-4-carboxamide ribonucleciside (AICAR) or clenbuterol, which are 5&apos;-AMP-activated protein kinase (AMPK) and beta 2-adrenergic receptor (beta 2-AR) activators, respectively. These results may suggest that enhanced expression of lipin-1 is involved in exercise-induced mitochondrial enzyme adaptations, possibly through AMPK- and beta 2-AR-related mechanisms. (C) 2008 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2008.07.079

    Web of Science

  • 異なる脂肪酸組成の高脂肪食摂取がラット骨格筋のミトコンドリア酵素活性およびPGC-1α蛋白含量に及ぼす影響

    東田 一彦, 三上 恵里, 園生 智広, 樋口 満, 寺田 新

    日本運動生理学雑誌   15 ( 2 )   45 - 51   2008年

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    記述言語:日本語   出版者・発行元:日本運動生理学会事務局  

    The purposes of this study were 1) to examine whether fatty acid composition of the diet might affect mitochondrial enzyme activities in rat skeletal muscle, and 2) to assess the effects of the high-fat diets on skeletal muscle peroxisome proliferator activated receptor γ coactivator-1α (PGC-1α) protein content, which is a key regulator of adaptive mitochondrial biogenesis. Four-week-old male Sprague-Dawley rats were fed for 30 days either a rodent chow (12% calorie from fat, CON group; n=5) or a high-fat diet (50% calorie from fat) containing lard (LARD group; n=5) or olive oil (OLIVE group; n=5). Citrate synthase (CS) and 3-hydroxyacyl-CoA dehydrogenase (3-HAD) activities in plantaris muscles were significantly higher in both LARD and OLIVE groups than CON group (p<0.05). Furthermore, the 3-HAD activity in OLIVE group was significantly higher than that in LARD group (p<0.05). PGC-1α protein contents in epitrochlearis muscle were significantly higher in both LARD and OLIVE groups than CON group by 88 and 70%, respectively(p<0.05). No significant difference in PGC-1α protein content was observed between LARD and OLIVE groups. In conclusion, these results indicate that 1) olive oil, which consists mostly of unsaturated fatty acids, has more pronounced effects on the fatty acid oxidation enzyme activity in skeletal muscle, compared with lard enriched in saturated fatty acids, and 2) feeding high-fat diets increases transcription coactivator PGC-1α protein content in rat skeletal muscle.

    CiNii Books

    その他リンク: http://dl.ndl.go.jp/info:ndljp/pid/10563281

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受賞

  • 第16回日本運動生理学会大会Young Investigator Award 優秀賞

    2008年8月  

共同研究・競争的資金等の研究課題

  • 骨格筋タンパク代謝における鉄の役割の解明

    研究課題/領域番号:20K11364  2020年4月 - 2023年3月

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    東田 一彦

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    配分額:4420000円 ( 直接経費:3400000円 、 間接経費:1020000円 )

  • 運動パフォーマンス向上のための脂質を用いた新たな栄養学的介入方法の開発

    研究課題/領域番号:17K13188  2017年4月 - 2020年3月

    日本学術振興会  科学研究費助成事業 若手研究(B)  若手研究(B)

    東田 一彦

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    配分額:4030000円 ( 直接経費:3100000円 、 間接経費:930000円 )

    本研究では、高脂肪食を摂取することで骨格筋の基質酸化能が向上すること、さらに、高脂肪食摂取を中断することで急速に適応反応が消失することを明らかにした。また、低糖質食の摂取は、腸管での糖吸収速度を低下させること、さらに運動後の筋グリコーゲン回復に悪影響を及ぼす可能性が明らかとなった。一方、肝グリコーゲンの回復速度を高めることも併せて明らかになった。脂質や糖質摂取量の調節による、運動パフォーマンス向上を目的とした栄養摂取方法の開発に有用な基礎的知見を得た。

  • 持久性運動開始前の糖質摂取は本当にインスリン・ショックを引き起こすか

    研究課題/領域番号:15K12673  2015年4月 - 2017年3月

    日本学術振興会  科学研究費助成事業 挑戦的萌芽研究  挑戦的萌芽研究

    樋口 満, 坂本 静男, 田口 素子, 東田 一彦

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    配分額:2470000円 ( 直接経費:1900000円 、 間接経費:570000円 )

    運動前の糖質摂取によって引き起こされるインスリンショック(運動誘発性低血糖)と呼ばれる現象について、主に朝食摂取の有無による違いに着目し研究を行った。本研究の結果、絶食条件だけではなく、実際のスポーツ現場に近い状態である朝食を摂取した条件においても、運動誘発性低血糖を発症する場合があることが明らかになった。さらに、低血糖の発症のしやすさには個人差が認められ、絶食条件においては、高いインスリン分泌能を有している者が、また朝食摂取条件においては、高い有酸素性能力を有している者が、運動誘発性低血糖を生じやすいことが明らかとなった。

  • 運動が乳ガン発症を予防する分子機序の解明

    研究課題/領域番号:25560360  2013年4月 - 2016年3月

    日本学術振興会  科学研究費助成事業 挑戦的萌芽研究  挑戦的萌芽研究

    東田 一彦

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    配分額:3770000円 ( 直接経費:2900000円 、 間接経費:870000円 )

    近年の大規模疫学研究により、習慣的な身体活動が乳ガン発症や再発を低下させることが明らかとなってきている。しかし、「なぜ身体活動は乳ガンの発症を減らすことができるのか?」という、分子機序は明らかではない。身体運動を行うことで骨格筋から様々な分子が分泌されていることが近年明らかにされているため、本研究では、身体活動による乳ガン抑制効果に骨格筋由来因子が関与しているとの仮説を立て、骨格筋由来因子がガン細胞の増殖能に及ぼす影響を検討した。本研究の結果、骨格筋由来因子CTRP15は乳ガン細胞のエネルギー代謝を調節することで増殖能を低下させる可能性が考えられる。

  • 筋内脂肪滴合成・分解の分子メカニズムの解明

    研究課題/領域番号:24800076  2012年8月 - 2014年3月

    日本学術振興会  科学研究費助成事業 研究活動スタート支援  研究活動スタート支援

    東田 一彦

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    配分額:2990000円 ( 直接経費:2300000円 、 間接経費:690000円 )

    骨格筋の脂肪滴分解関連タンパク質は、骨格筋細胞内での脂肪蓄積を調節していると考えられ、インスリン抵抗性の発症に関与している可能性が示唆されている。本年度は、運動による脂肪滴関連蛋白質遺伝子発現調節に関して検討を行った。糖尿病予防に効果的とされている低強度・長時間運動を行ったラットの骨格筋において、脂肪分解関連タンパク質であるPerilipin 5、Adipose Triglycerol lipase(ATGL)やHormone Sensitive Lipase(HSL)が増加することが明らかとなった。一方、ATGLのアクチベーターであるCGI-58のタンパク量は変化しないことも併せて明らかとなった。
    脂肪分解活性の調節メカニズムに関しては、Myc-Perilipin 5発現プラスミドをエレクトロポレーションにより骨格筋で高発現させる実験系の確立を試みた。本年度は、遺伝子導入から1週間目に骨格筋を摘出し、Mycに対する抗体で発現量を確認した。その結果、Mycの発現が遺伝子導入足のみで確認された。来年度以降は、この系を用いて、Perilipin 5を中心に、骨格筋の脂肪分解活性調節メカニズムを明らかにし、それを効率的に高める、運動プログラムの開発や、機能性食品のスクリーニングに発展させる。

  • 身体運動による骨格筋のミトコンドリア増加メカニズムの解明

    研究課題/領域番号:10J02904  2010年 - 2011年

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    東田 一彦

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    配分額:1900000円 ( 直接経費:1900000円 )

    骨格筋のミトコンドリア機能不全が、骨格筋細胞内の脂質代謝を低下させ、インスリン抵抗性を引き起こす原因と考えられている。一方、身体運動により骨格筋のミトコンドリアが増加し、脂質代謝が亢進することが知られているものの、その詳細な分子メカニズムは明らかではない。近年、肝臓において転写補助因子Lipin-1がミトコンドリア新生を引き起こすことが報告された。そこで本研究では骨格筋でのLipin-1の機能、さらに身体運動によるミトコンドリア増加にLipin-1が関与しているかを検討した。本年度の研究においては、Lipin-1骨格筋培養細胞において効率的に遺伝子導入を行うために、Lipin-1遺伝子をコードしたアデノウイルスを作成した。マウス由来のC2C12培養細胞にLipin-1アデノウイルスを作用させ、Lipin-1を強発現させたところ、ミトコンドリアComplex IV Subunit 1、NADH ubiquinone oxidoreductaseおよびComplex II subunit 30kDaなどのミトコンドリアタンパク質の増加が認められた。したがって、骨格筋においてもLipin-1はミトコンドリアを増加させる機能を有していることが確認された。さらに、PGC-1αとGAL4DNA結合ドメイン融合タンパク発現プラスミド、GAL4結合配列を含むルシフェラーゼレポータープラスミドおよびLipin-1プラスミドをC2C12細胞に発現させ、PGC-1αの転写活性能を測定した。その結果、PGC-1α活性がLipin-1により増加することが明らかとなった。

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担当経験のある科目(授業)

  • 運動と健康

    2015年4月 - 現在 機関名:滋賀県立大学

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    科目区分:学部専門科目 

  • 健康・体力科学Ⅱ

    2015年4月 - 現在 機関名:滋賀県立大学

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    科目区分:学部全学共通科目 

  • 健康・体力科学Ⅰ

    2015年4月 - 現在 機関名:滋賀県立大学

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    科目区分:学部全学共通科目