研究者詳細 - 浦部　美佐子

論文 - 浦部　美佐子

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Integrative approach for discovering of the new species within the genus Allocreadium Looss, 1900 (Trematoda: Allocreadiidae) and framing of biogeographical hypotheses for the genus 査読

Vainutis K.S., Voronova A.N., Urabe M., Kazarin V.M.

Systematic Parasitology 2022年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Systematic Parasitology

In July 2012 new Allocreadium species was isolated from Carassius gibelio caught in the Arsenyevka River, Primorsky region, Russia. Analyses on the morphometrics and internal organs′ topology revealed that these worms are morphologically closest with A. isoporum but both species are independent according to high genetic distances based on the 28S gene fragment (5.434±0.0073%). Unlike A. isoporum found earlier in Europe, the new species named A. pseudoisoporumsp. nov. has a shorter body length and the vitellarium not reaching the posterior end of the body at some distance and its anterior border is on the level of the ventral sucker. Allocreadium pseudoisoporumsp. nov. differs from seven species previously found in the Russian Far East with the following features: smaller size of the body, suckers′ ratio, range values, and topology of internal structures. Newly localities in the Pavlovka River and the Artyomovka River were discovered for A. khankaiensis. Morphological variability of the worms from the Pavlovka River was observed in comparison with A. khankaiensis from the Komissarovka River. Using scanning electron microscope, we examined external surfaces of three species (A. pseudoisoporumsp. nov., A. khankaiensis, A. hemibarbi) and observed structures reminiscent sensory receptors. This study was aimed to describe species diversity of allocreadiids inhabiting the south of Primorsky region, and to clarify phylogenetic relationships between the species from the genus Allocreadium Looss, 1900 using molecular genetic methods. The phylogenetic Bayesian tree based on the 28S gene showed a clear separation of ten Allocreadium species and confirmed the validity of A. pseudoisoporumsp. nov.Allocreadium pseudoisoporumsp. nov. is most similar to A. gotoi (genetic distances – 3.578±0.0051% in 28S, and 18.777±0.0149% in cox1), and represented the earliest divergent lineage in Allocreadium clade on the phylogenetic tree based on the 28S gene, thereby indicating its proximity to the ancestral node. Also, dichotomous keys for 25 Palearctic species of Allocreadium were prepared based on the morphology of the adult worms.

DOI： 10.1007/s11230-022-10081-1

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琵琶湖沿岸に生息するエビノコバンTachaea chinensisの宿主利用. 査読

前田陽也，浦部美佐子

陸水研究 8 2022年

Three species of Exorchis Kobayashi, 1921 (Digenea: Cryptogonimidae) in the East-Asian region: Morphological and molecular data 査読

Solodovnik D.A., Tatonova Y.V., Urabe M., Besprozvannykh V.V., Nakao M., Inoue K.

Parasitology 148 ( 13 ) 1578 - 1587 2021年11月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Parasitology

New data have been obtained for three representatives of Exorchis; Exorchis convictus sp. n., Exorchis oviformis and Exorchis sp., from fish in the East-Asian region. For the first time, based on combined sequences of the ITS2 rDNA region and the 28S rRNA gene, Exorchis is confirmed to belong Cryptogonimidae. Based on analysis of a mitochondrial marker (cox1), the 'Japanese' and 'Russian' haplogroups are identified for E. oviformis isolated from Silurus asotus. One specimen of E. oviformis obtained in Japan is identical to the 'Russian' haplotype. Haplotype patterns are also observed for metacercariae of Exorchis sp. from Tanakia lanceolata and Carassius sp. fish in Kyushu Island (Japan).

DOI： 10.1017/S0031182021001232

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Systematics of Crepidostomum species from the Russian Far East and northern Japan, with description of a new species and validation of the genus Stephanophiala 査読

Vainutis K.S., Voronova A.N., Urabe M.

Parasitology International 84 2021年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Parasitology International

Current article touched upon the issue of the complicated taxonomic status of some species from the genus Crepidostomum collected from the freshwater fish in the rivers of Primorsky region, Sakhalin, and Hokkaido Islands. Primary morphological analyses showed affiliation of the worms to the species C. farionis (Müller, 1784) Lühe, 1909; C. metoecus Braun, 1900b; C. chaenogobii Yamaguti and Matsumura, 1942; C. nemachilus Krotov, 1959. We described the new species Crepidostomum achmerovi sp. nov. that is a sibling species of C. nemachilus. Molecular-genetic investigation have shown that C. nemachilus and C. achmerovi sp. nov. are closely related to C. metoecus in both 28S rDNA and cox1 mtDNA markers. Crepidostomum nemachilus forms a separate branch within the C. metoecus clade on the 28S BI tree with strong statistical support and separate clade in relation to C. metoecus clade on the cox1 BI tree. Values of p-distances between Crepidostomum species were at intergeneric level. Crepidostomum metoecus species complex including five species (C. metoecus, C. nemachilus, C. oschmarini, C. brinkmanni, and C. achmerovi sp. nov.) was reconsidered as independent genus Crepidostomum sensu stricto. Minimum Spanning Network showed that C. nemachilus, C. metoecus and C. achmerovi sp. nov. were separated by large number of mutational events and represent independent phyletic lines. An amended diagnosis is provided for the subfamily Crepidostomatinae, the genera Crepidostomum s. str. and Stephanophiala Nicoll, 1909, along with keys to species of both genera.

DOI： 10.1016/j.parint.2021.102412

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A new species of hemiuroidean trematode from Hatcheria macraei (Siluriformes, Trichomycteridae) and Heleobia hatcheri (Gastropoda, Cochliopidae) in a Patagonian River 査読

Tsuchida K., Urabe M., Viozzi G., Rauque C., Flores V.

Parasitology Research 120 ( 7 ) 2523 - 2532 2021年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Parasitology Research

A new hemiuroidean species, Genarchella pichileufuensis n. sp. (Derogenidae: Halipeginae), was found in the stomach of the siluriform freshwater fish, Hatcheria macraei (Girard, 1855), in the Pichileufu River, Patagonia, Argentina. Its rediae with immature cystophorous cercariae were found in the snail Heleobia hatcheri (Pilsbry, 1911) in the same site. The present new species is morphologically featured by having a cyclocoel in the hindbody unlike the other species of the genus. The characteristics of this species allowed us to amend the diagnosis of the genus Genarchella as follows: cyclocoel present or absent; testes symmetrical to tandem; ootype pouch present. In the phylogenetic analysis, G. pichileufuensis forms a well-supported clade with Genarchella spp. recovered from Mexican freshwater fishes. This clade is included in the cluster of representatives of the subfamily Halipeginae. So far, three hemiuroidean species, Thometrema patagonica (Szidat, 1956), Derogenes lacustris Tsuchida, Flores, Viozzi, Rauque et Urabe, 2021 and G. pichileufuensis n. sp., have been reported from freshwater fishes in Argentinean Patagonia.

DOI： 10.1007/s00436-021-07210-y

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Hemiuroidean trematodes from freshwater Patagonian fishes: description of a new species, distribution and molecular phylogeny 査読

Tsuchida K., Flores V., Viozzi G., Rauque C., Urabe M.

Parasitology Research 120 ( 4 ) 1219 - 1232 2021年4月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Parasitology Research

A new trematode species, Derogenes lacustris Tsuchida, Flores, Viozzi, Rauque et Urabe n. sp. (Derogenidae: Derogeninae), from freshwater fishes is described using morphological and molecular approaches in Argentinean Patagonia. D. lacustris is the most common hemiuroidean species in the Limay River basin and parasitizes almost all the native and introduced Patagonian freshwater fish. This new species could be considered as the unique freshwater species in the genus Derogenes Nicoll, 1910. Another hemiuroidean species, Thometrema patagonica Szidat (Archiev Hydrobiol 51: 542–577, 1956) Lunaschi et Drago 2000 (Derogenidae: Halipeginae), is found from Percichthys trucha (Perciformes) in the Neuquén River basin. Its diagnosis and molecular data are provided by the present study. In the molecular analysis of the Patagonian hemiuroideans, T. patagonica composes a group with halipeginean species in the phylogenetic tree of 28S rDNA sequences, while D. lacustris is not included in the same group. D. lacustris also shows low intraspecific variation in COI sequences regardless of the localities or host species.

DOI： 10.1007/s00436-020-06939-2

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The first survey for helminths parasitic in hybrid and introduced giant salamanders, genus Andrias (Amphibia: Caudata: Cryptobranchidae) in Kyoto, Japan 査読

Tsuchida, K., Urabe, M., Nishikawa, K. (2021)

Current Herpetology 40 109 - 119 2021年

Paraharmotrema karinganiense n. gen., n. sp. (Digenea: Liolopidae) infecting the intestine of serrated hinged terrapin (Pelusios sinuatus), east African black mud turtle (Pelusios subniger), and South African helmeted turtle (Pelomedusa galeata) and a phy 査読国際誌

Dutton, H. A., L. H. DuPreez, M. Urabe, S. A. Bullard

Journal for Parasitology: Parasites and Wildlife 17 43 - 52 2021年

First report of post-cyclic transmission in trematodes: Derogenes lacustris (Digenea: Hemiuroidea) in Patagonian freshwater fishes. 査読国際誌

Tsuchida, K., C. Rauque, G. Viozzi, V. Flores, M. Urabe

Parasitology 18 2021年

Ancient drainage networks mediated a large-scale genetic introgression in the East Asian freshwater snails 査読国際誌

Miura, O., Urabe, M., Mori, H., Chiba, S.

Ecology and Evolution 2020年7月

記述言語：英語掲載種別：研究論文（学術雑誌）参加形態：共同（副担当）

Biogeography and genetic variation of freshwater organisms are influenced not only by current freshwater connections but also by past drainage networks. The Seto Inland Sea is a shallow enclosed sea in Japan, but geological evidence showed that a large freshwater drainage had intermittently appeared in this area between the late Pliocene and Pleistocene. Here, we demonstrated that this paleodrainage greatly affected the genetic variation of the East Asian freshwater snails, Semisulcospira spp. We found that the mtDNA haplotypes originated in the Lake Biwa endemic Semisulcospira species at the upstream side of the paleodrainage were frequently observed in the riverine Semisulcospira species at its downstream side. The genome-wide DNA and morphological analyses consistently showed that there was no clear evidence of nuclear introgression between the Lake Biwa endemics and riverine species. These results suggest that the large paleodrainage had facilitated mitochondrial introgression and had broadly spread the introgressed mtDNA haplotypes to its downstream region around the Seto Inland Sea. Our study highlights the role of paleodrainages in shaping the genetic variation of freshwater organisms.

DOI： 10.1002/ece3.6523

その他リンク： https://onlinelibrary.wiley.com/doi/full/10.1002/ece3.6523

Description and molecular characteristics of Morishitium polonicum malayense Urabe, Nor Hashim & Uni, n. subsp. (Trematoda: Cyclocoelidae) from the Asian glossy starling, Aplonis panayensis strigata (Passeriformes: Sturnidae) in Peninsular Malaysia. 査読国際誌

Urabe, M., Nor Hashim, N. E., Uni, S., et al.

Parasitology International 76 2020年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：日本寄生虫学会参加形態：共同（主担当）

We describe Morishitium polonicum malayense n. subsp. from Asian glossy starlings (Aplonis panayensis strigata) (Horsfield, 1821) (Passeriformis: Sturnidae) caught in Malaysia. The trematodes had parasitized the air sacs and the thoracic and body cavities of 40 out of 67 (59.7%) birds examined. The specimens each had an oral sucker, a postpharyngeal genital pore, and tandem testes, but lacked a ventral sucker. The morphological characteristics of our specimens were similar to those of M. polonicum polonicum (Machalska, 1980) from Poland. However, the anterior extremity of vitelline follicles of the present specimens sometimes extended to the level of pharynx. The oral sucker width, oral sucker width/pharynx width ratio, and intertesticular space metrics differed from those of M. p. polonicum. The maximum-likelihood trees based on the cytochrome c oxidase subunit I (COI) and the internal transcribed spacer 2 (ITS2) sequences indicated that the species from the present study formed a sister group with M. p. polonicum from the Czech Republic. The p-distances of COI and ITS2 sequences between the present specimens and M. p. polonicum from the Czech Republic were 6.9–7.5% and 0.6%, respectively. These genetic divergences indicate the border for intra- or interspecific variation of digeneans. The definitive host species and geographical distribution of the current specimens were distinct from those of M. p. polonicum from Europe. We thus concluded that the present specimens are ranked as a new subspecies of M. polonicum, namely M. polonicum malayense n. subsp.

DOI： 10.1016/j.parint.2020.102074

Do atypical 15N and 13C enrichment in parasites result from isotope ratio variation of host tissues they are infected? 査読国際誌

Kamiya, E., Urabe, M. Okuda, N.

Limnology 21 139 - 149 2020年1月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：日本陸水学会参加形態：共同（主担当）

Contrary to a general rule of stable isotope trophic discrimination, parasites show great variation in isotope discrimination from their host muscle tissues. One of the possible mechanisms is that isotopic variation among host tissues may cause apparent deviation from the actual isotope discrimination between a parasite and a given host tissue consumed by the parasite. To test this possibility, we measured δ15N and δ13C for some fish parasites and a variety of their host tissues to estimate their actual trophic discrimination factors (Δ). All of the trematodes investigated showed positive Δ15Ν values and nearly zero Δ13C values, which are comparable to those of free-living consumers. Cestodes and nematodes showed great variations in their Δ15Ν and Δ13C values when they were compared to a particular tissue/mucus of their infection site. Acanthocephalans showed negative Δ15Ν and Δ13C values in comparison with host intestinal mucus, and a crustacean had δ values similar to those of host blood. Even in parasites infected to intestine, Δ15Ν and Δ13C values varied among higher taxa. These results suggested that atypical 15N and 13C enrichment of parasites is not caused by the variation in δ values among host tissues, but possibly by metabolic processes unique to each parasite taxon.

Morphological and molecular studies of Eudiplozoon nipponicum (Goto, 1891) and Eudiplozoon kamegaii sp. n. (Monogenea; Diplozoidae). 査読国際誌

Nishihira, T., Urabe, M

Folia Parasitologica 67 2020年

The curious case of the endemic freshwater crocodile Crocodylus mindorensis as incidental host of marine fish acanthocephalan 査読国際誌

Marcaida, A. J. B., Urabe, M., Briones, J. C. A., Diesmos, M. L. L., Tellez, M, Diesmos, A. C.

Parasitology International 72 2019年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：日本寄生虫学会参加形態：共同（副担当）

We performed the first host-parasite survey of the Philippine crocodile, Crocodylus mindorensis, a critically endangered species for which ecological information is lacking. We collected by gastric lavage samples of the stomach contents of crocodiles (n = 10) residing at the Palawan Wildlife Rescue and Conservation Center in Puerto Princesa, Palawan, Philippines. The only parasite detected was an acanthocephalan, which was identified as Neorhadinorhynchus nudus (n = 68), a parasite typically found in the marine fish species consumed by three crocodile individuals. Given the known hosts of N. nudus, its parasitism of C. mindorensis in captivity is likely established by consumption of marine fish. Our findings have implications for the conservation management of C. mindorensis, particularly in terms of preventing introduction of parasites that could lead to development of infectious disease or alter the fitness of captive animals.

DOI： 10.1016/j.parint.2019.101940

その他リンク： https://www.sciencedirect.com/science/article/pii/S1383576919300078?via%3Dihub

Redescription and supplementary molecular characteristics of Aspidogaster ijimai Kawamura, 1915 (Trematoda, Aspidogastrea, Aspidogastridae), a parasite of Cyprinus carpio Linnaeus, 1758 s. lato (Actinopterygii) and freshwater bivalves in East Asia 査読国際誌

S.G.Sokolov, D.M.Atopkin, M.Urabe

Parasitology International 71: 71 167 - 176 2019年8月

記述言語：英語掲載種別：研究論文（学術雑誌）参加形態：共同（副担当）

A detailed morphological description and ribosomal DNA (rDNA) sequence molecular data for Aspidogaster ijimai from Japan are provided. Morphological analysis, including a description of the cirrus-sac, indicate the conspecificity of Japanese and continental East Asian A. ijimai specimens. Analysis of ITS1-5.8S-ITS2 rDNA sequences of Japanese, Chinese and Russian specimens confirmed the morphological results. Phylogenetic analysis using ITS rDNA sequences confirmed that A. ijimai is a sister species for Aspidogaster chongqingensis. Median-joining network analysis showed an initial molecular differentiation step of Russian specimens from group of Japanese-Chinese samples. Our 28S rDNA results based on maximum likelihood (ML) and Bayesian (BI) phylogenetic analyses indicated well-supported monophyly of the Aspidogaster conchicola + A. ijimai group, a finding that indicates that these species are congeneric. At the same time, our data demonstrated that the genus Lobatostoma is paraphyletic and the family Aspidogastridae is polyphyletic, results that confirm previous studies.

その他リンク： https://www.sciencedirect.com/science/article/pii/S1383576919300480

Phylogenetic analysis of the superfamily Hemiuroidea (Platyhelminthes, Neodermata: Trematoda) based on partial 28S rDNA sequences 査読

Sokolov S., Atopkin D., Urabe M., Gordeev I.

Parasitology 146 ( 5 ) 596 - 603 2019年4月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Parasitology

© Cambridge University Press 2018. In the present paper, the phylogenetic relationships between genera, subfamilies and families of the Hemiuroidea are explored. Twelve new sequences of 28 rDNA and data taken from GenBank (NSBI) on 43 species affiliated to 34 genera were included in the analysis. Most of the hemiuroidean trematodes form two highly supported clades (A and B), which are sister groups to each other. Hemipera manteri joined with Gonocerca spp. with moderate statistical support. This clade is basal relative to the clades A and B. Dilade A is polytomic and contains representatives of the families Accacoeliidae, Syncoeliidae, Didymozoidae, Hirudinellidae and Sclerodistomidae, and derogenid subfamilies Derogeninae and Halipeginae. At the same time, the Syncoeliidae, Hirudinellidae and Accacoeliidae form a well-supported monophyletic group. The phylogenetic relationship between Derogeninae and Halipeginae is poorly resolved. Dilade B unites the isoparorchiid, bunocotylid, lecithasterid and hemiurid trematodes. Our data re-establishes the family Bunocotylidae, which consists of two subfamilies, Opisthadeninae and Bunocotylinae, and the Machidatrema chilostoma + Hysterolecithoides frontilatus group. The Bunocotylidae is the sister group to the Hemiuridae + Lecithasteridae group and the Isoparorchiidae is a basal relative to the representatives of these three hemiuroid families.

DOI： 10.1017/S0031182018001841

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Long-term dynamics of Metagonimus spp. (Trematoda; Digenea) in ayu fish (Plecoglossus altivelis) in Shizuoka Prefecture, Japan. 査読

Urabe, M. and Kino, H

陸水研究 5 00 - 00 2019年