研究者詳細 - 辰巳佐和子

論文 - 辰巳佐和子

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辰巳佐和子, 瀬川博子, 宮本賢一

日本透析医会雑誌 35 ( 1 ) 162 - 167 2020年4月

記述言語：日本語出版者・発行元：(公社)日本透析医会

血中リン濃度は、主に腸管吸収、骨代謝(骨形成・吸収)、腎臓における排泄と再吸収、肝臓、筋肉といった軟組織への組織移行により維持される。血中リン濃度は日内リズムを形成し、その形成機序は早朝空腹時のリン濃度を規定する重要な要因と考えられている。慢性腎臓病(CKD)では非常に早期よりリン代謝異常が生じている。CKDや維持透析患者の死亡リスクは、早朝空腹時の血中リン濃度と相関することが知られている。最近、Nampt(nicotinamide phosphoribosyl transferase)/NAD(nicotinamide adenine dinucleotide)系が、ナトリウム依存性リン輸送体であるNaPi-IIa(Npt2a)、NaPi-IIc(Npt2c)、NaPi-IIb(Npt2b)の発現量を調節し、腎臓リン排泄、腸管リン吸収を調節することで、血中リン濃度の日内リズム形成に関わることが明らかにされた。実際に、Namptヘテロ欠損マウスでは、血中リン濃度の日内リズムは消失する。また肝臓特異的Nampt欠損マウスでは、異常な日内リズム形成を示すことから、リンの組織移行にも関与する可能性が示唆されている。生体におけるリン代謝調節とその日内リズム形成のさらなる理解は、CKDや維持透析患者のリン管理において重要である。(著者抄録)

Role of sodium-dependent Pi transporter/Npt2c on Pi homeostasis in klotho knockout mice different properties between juvenile and adult stages. 査読国際誌

Hanazaki A, Ikuta K, Sasaki S, Sasaki S, Koike M, Tanifuji K, Arima Y, Kaneko I, Shiozaki Y, Tatsumi S, Hasegawa T, Amizuka N, Miyamoto KI, Segawa H.

Physiol Rep. 8 ( 3 ) e14324 2020年2月

Role of sodium-dependent Pi transporter/Npt2c on Pi homeostasis in klotho knockout mice different properties between juvenile and adult stages. 査読国際誌

Ai Hanazaki, Kayo Ikuta, Shohei Sasaki, Sumire Sasaki, Megumi Koike, Kazuya Tanifuji, Yuki Arima, Ichiro Kaneko, Yuji Shiozaki, Sawako Tatsumi, Tomoka Hasegawa, Norio Amizuka, Ken-Ichi Miyamoto, Hiroko Segawa

Physiological reports 8 ( 3 ) e14324 2020年2月

記述言語：英語掲載種別：研究論文（学術雑誌）

SLC34A3/NPT2c/NaPi-2c/Npt2c is a growth-related NaPi cotransporter that mediates the uptake of renal sodium-dependent phosphate (Pi). Mutation of human NPT2c causes hereditary hypophosphatemic rickets with hypercalciuria. Mice with Npt2c knockout, however, exhibit normal Pi metabolism. To investigate the role of Npt2c in Pi homeostasis, we generated α-klotho-/- /Npt2c-/- (KL2cDKO) mice and analyzed Pi homeostasis. α-Klotho-/- (KLKO) mice exhibit hyperphosphatemia and markedly increased kidney Npt2c protein levels. Genetic disruption of Npt2c extended the lifespan of KLKO mice similar to that of α-Klotho-/- /Npt2a-/- mice. Adult KL2cDKO mice had hyperphosphatemia, but analysis of Pi metabolism revealed significantly decreased intestinal and renal Pi (re)absorption compared with KLKO mice. The 1,25-dihydroxy vitamin D3 concentration was not reduced in KL2cDKO mice compared with that in KLKO mice. The KL2cDKO mice had less severe soft tissue and vascular calcification compared with KLKO mice. Juvenile KL2cDKO mice had significantly reduced plasma Pi levels, but Pi metabolism was not changed. In Npt2cKO mice, plasma Pi levels began to decrease around the age of 15 days and significant hypophosphatemia developed within 21 days. The findings of the present study suggest that Npt2c contributes to regulating plasma Pi levels in the juvenile stage and affects Pi retention in the soft and vascular tissues in KLKO mice.

DOI： 10.14814/phy2.14324

PubMed

Role of the putative PKC phosphorylation sites of the type IIc sodium-dependent phosphate transporter in parathyroid hormone regulation. 査読国際誌

Fujii T, Segawa H, Hanazaki A, Nishiguchi S, Minoshima S, Ohi A, Tominaga R, Sasaki S, Tanifuji K, Koike M, Arima Y, Shiozaki Y, Kaneko I, Ito M, Tatsumi S, Miyamoto KI.

Clin Exp Nephrol. 23 ( 7 ) 898 - 907 2019年7月

Role of the putative PKC phosphorylation sites of the type IIc sodium-dependent phosphate transporter in parathyroid hormone regulation. 査読

Fujii T, Segawa H, Hanazaki A, Nishiguchi S, Minoshima S, Ohi A, Tominaga R, Sasaki S, Tanifuji K, Koike M, Arima Y, Shiozaki Y, Kaneko I, Ito M, Tatsumi S, Miyamoto KI

Clinical and experimental nephrology 23 ( 7 ) 898 - 907 2019年7月

リン代謝における肝臓Nampt/NAD経路の役割

辰巳佐和子, 中辻翔也, 斎満帆, 桑原頌治, 金子一郎, 瀬川博子, 宮本賢一

日本栄養・食糧学会大会講演要旨集 73回 296 - 296 2019年4月

Analysis of opossum kidney NaPi-IIc sodium-dependent phosphate transporter to understand Pi handling in human kidney. 査読国際誌

Fujii T, Shiozaki Y, Segawa H, Nishiguchi S, Hanazaki A, Noguchi M, Kirino R, Sasaki S, Tanifuji K, Koike M, Yokoyama M, Arima Y, Kaneko I, Tatsumi S, Ito M, Miyamoto KI.

Clin Exp Nephrol. 2019年3月

Analysis of opossum kidney NaPi-IIc sodium-dependent phosphate transporter to understand Pi handling in human kidney. 査読

Fujii T, Shiozaki Y, Segawa H, Nishiguchi S, Hanazaki A, Noguchi M, Kirino R, Sasaki S, Tanifuji K, Koike M, Yokoyama M, Arima Y, Kaneko I, Tatsumi S, Ito M, Miyamoto KI

Clinical and experimental nephrology 23 ( 3 ) 313 - 324 2019年3月

Systemic network for dietary inorganic phosphate adaptation among three organs. 査読国際誌

Ikuta K, Segawa H, Hanazaki A, Fujii T, Kaneko I, Shiozaki Y, Tatsumi S, Ishikawa Y, Miyamoto KI.

Pflugers Arch 2019年1月

NAD metabolism and the SLC34 family: evidence for a liver-kidney axis regulating inorganic phosphate. 査読

Tatsumi S, Katai K, Kaneko I, Segawa H, Miyamoto KI

Pflugers Archiv : European journal of physiology 471 ( 1 ) 109 - 122 2019年1月

Systemic network for dietary inorganic phosphate adaptation among three organs. 査読国際誌

Ikuta K, Segawa H, Hanazaki A, Fujii T, Kaneko I, Shiozaki Y, Tatsumi S, Ishikawa Y, Miyamoto KI

Pflugers Archiv : European journal of physiology 471 ( 1 ) 123 - 136 2019年1月

A Role of Intestinal Alkaline Phosphatase 3 (Akp3) in Inorganic Phosphate Homeostasis. 査読国際誌

Sasaki S, Segawa H, Hanazaki A, Kirino R, Fujii T, Ikuta K, Noguchi M, Sasaki S, Koike M, Tanifuji K, Shiozaki Y, Kaneko I, Tatsumi S, Shimohata T, Kawai Y, Narisawa S, Millán JL, Miyamoto KI.

Kidney Blood Press Res. 2018年9月

Eldecalcitol Causes FGF23 Resistance for Pi Reabsorption and Improves Rachitic Bone Phenotypes in the Male Hyp Mouse. 査読国際誌

Kaneko I, Segawa H, Ikuta K, Hanazaki A, Fujii T, Tatsumi S, Kido S, Hasegawa T, Amizuka N, Saito H, Miyamoto KI.

Endocrinology 2018年7月

Eldecalcitol Causes FGF23 Resistance for Pi Reabsorption and Improves Rachitic Bone Phenotypes in the Male Hyp Mouse. 査読国際誌

Kaneko I, Segawa H, Ikuta K, Hanazaki A, Fujii T, Tatsumi S, Kido S, Hasegawa T, Amizuka N, Saito H, Miyamoto KI

Endocrinology 159 ( 7 ) 2741 - 2758 2018年7月

Effect of Npt2b deletion on intestinal and renal inorganic phosphate (Pi) handling 査読

Kayo Ikuta, Hiroko Segawa, Shohei Sasaki, Ai Hanazaki, Toru Fujii, Aoi Kushi, Yuka Kawabata, Ruri Kirino, Sumire Sasaki, Miwa Noguchi, Ichiro Kaneko, Sawako Tatsumi, Otoya Ueda, Naoko A. Wada, Hiromi Tateishi, Mami Kakefuda, Yosuke Kawase, Shuichi Ohtomo, Yasuhiro Ichida, Akira Maeda, Kou-ichi Jishage, Naoshi Horiba, Ken-ichi Miyamoto

Clinical and Experimental Nephrology 22 ( 3 ) 517 - 528 2018年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Springer Tokyo

Background: Hyperphosphatemia is common in chronic kidney disease and is associated with morbidity and mortality. The intestinal Na+-dependent phosphate transporter Npt2b is thought to be an important molecular target for the prevention of hyperphosphatemia. The role of Npt2b in the net absorption of inorganic phosphate (Pi), however, is controversial. Methods: In the present study, we made tamoxifen-inducible Npt2b conditional knockout (CKO) mice to analyze systemic Pi metabolism, including intestinal Pi absorption. Results: Although the Na+-dependent Pi transport in brush-border membrane vesicle uptake levels was significantly decreased in the distal intestine of Npt2b CKO mice compared with control mice, plasma Pi and fecal Pi excretion levels were not significantly different. Data obtained using the intestinal loop technique showed that Pi uptake in Npt2b CKO mice was not affected at a Pi concentration of 4 mM, which is considered the typical luminal Pi concentration after meals in mice. Claudin, which may be involved in paracellular pathways, as well as claudin-2, 12, and 15 protein levels were significantly decreased in the Npt2b CKO mice. Thus, Npt2b deficiency did not affect Pi absorption within the range of Pi concentrations that normally occurs after meals. Conclusion: These findings indicate that abnormal Pi metabolism may also be involved in tight junction molecules such as Cldns that are affected by Npt2b deficiency.

DOI： 10.1007/s10157-017-1497-3

Scopus

PubMed

The sodium phosphate cotransporter family and nicotinamide phosphoribosyltransferase contribute to the daily oscillation of plasma inorganic phosphate concentration 査読

Atsumi Miyagawa, Sawako Tatsumi, Wako Takahama, Osamu Fujii, Kenta Nagamoto, Emi Kinoshita, Kengo Nomura, Kayo Ikuta, Toru Fujii, Ai Hanazaki, Ichiro Kaneko, Hiroko Segawa, Ken-ichi Miyamoto

Kidney International 93 ( 5 ) 1073 - 1085 2018年5月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Elsevier B.V.

Circulating inorganic phosphate exhibits a remarkable daily oscillation based on food intake. In humans and rodents, the daily oscillation in response to food intake may be coordinated to control the intestinal absorption, renal excretion, cellular shifts, and extracellular concentration of inorganic phosphate. However, mechanisms regulating the resulting oscillation are unknown. Here we investigated the roles of the sodium phosphate cotransporter SLC34 (Npt2) family and nicotinamide phosphoribosyltransferase (Nampt) in the daily oscillation of plasma inorganic phosphate levels. First, it is roughly linked to urinary inorganic phosphate excretion. Second, expression of renal Npt2a and Npt2c, and intestinal Npt2b proteins also exhibit a dynamic daily oscillation. Analyses of Npt2a, Npt2b, and Npt2c knockout mice revealed the importance of renal inorganic phosphate reabsorption and cellular inorganic phosphate shifts in the daily oscillation. Third, experiments in which nicotinamide and a specific Nampt inhibitor (FK866) were administered in the active and rest phases revealed that the Nampt/NAD+ system is involved in renal inorganic phosphate excretion. Additionally, for cellular shifts, liver-specific Nampt deletion disturbed the daily oscillation of plasma phosphate during the rest but not the active phase. In systemic Nampt+/− mice, NAD levels were significantly reduced in the liver, kidney, and intestine, and the daily oscillation (active and rest phases) of the plasma phosphate concentration was attenuated. Thus, the Nampt/NAD+ system for Npt2 regulation and cellular shifts to tissues such as the liver play an important role in generating daily oscillation of plasma inorganic phosphate levels.

DOI： 10.1016/j.kint.2017.11.022

Scopus

PubMed

The sodium phosphate cotransporter family and nicotinamide phosphoribosyltransferase contribute to the daily oscillation of plasma inorganic phosphate concentration. 国際誌

Miyagawa A, Tatsumi S*, Takahama W, Fujii O, Nagamoto K, Kinoshita E, Nomura K, Ikuta K, Fujii T, Hanazaki A, Kaneko I, Segawa H, Miyamoto KI*.

Kidney International 2018年2月

A Role of Intestinal Alkaline Phosphatase 3 (Akp3) in Inorganic Phosphate Homeostasis. 査読国際誌

Sasaki S, Segawa H, Hanazaki A, Kirino R, Fujii T, Ikuta K, Noguchi M, Sasaki S, Koike M, Tanifuji K, Shiozaki Y, Kaneko I, Tatsumi S, Shimohata T, Kawai Y, Narisawa S, Millán JL, Miyamoto KI

Kidney & blood pressure research 43 ( 5 ) 1409 - 1424 2018年

Effect of Osteocyte-Ablation on Inorganic Phosphate Metabolism: Analysis of Bone-Kidney-Gut Axis. 国際誌

Fujii O, Tatsumi S*, Ogata M, Arakaki T, Sakaguchi H, Nomura K, Miyagawa A, Ikuta K, Hanazaki A, Kaneko I, Segawa H, Miyamoto KI*.

Frontiers in Endocrinology 8 2017年12月

Effect of Osteocyte-Ablation on Inogranic Phosphate Metabolism: Analysis of Bone-Kidney-Gut Axis 査読

Osamu Fujii, Sawako Tatsumi, Mao Ogata, Tomohiro Arakaki, Haruna Sakaguchi, Kengo Nomura, Atsumi Miyagawa, Kayo Ikuta, Ai Hanazaki, Ichiro Kaneko, Hiroko Segawa, Ken-ichi Miyamoto

FRONTIERS IN ENDOCRINOLOGY 8 359 2017年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：FRONTIERS MEDIA SA

In response to kidney damage, osteocytes increase the production of several hormones critically involved in mineral metabolism. Recent studies suggest that osteocyte function is altered very early in the course of chronic kidney disease. In the present study, to clarify the role of osteocytes and the canalicular network in mineral homeostasis, we performed four experiments. In Experiment 1, we investigated renal and intestinal Pi handling in osteocyte-less (OCL) model mice [transgenic mice with the dentin matrix protein-1 promoter-driven diphtheria toxin (DT)-receptor that were injected with DT]. In Experiment 2, we administered granulocyte colony-stimulating factor to mice to disrupt the osteocyte canalicular network. In Experiment 3, we investigated the role of osteocytes in dietary Pi signaling. In Experiment 4, we analyzed gene expression level fluctuations in the intestine and liver by comparing mice fed a high Pi diet and OCL mice. Together, the findings of these experiments indicate that osteocyte ablation caused rapid renal Pi excretion (P < 0.01) before the plasma fibroblast growth factor 23 (FGF23) and parathyroid hormone (PTH) levels increased. At the same time, we observed a rapid suppression of renal Klotho (P < 0.01), type II sodium phosphate transporters Npt2a (P < 0.01) and Npt2c (P < 0.05), and an increase in intestinal Npt2b (P < 0.01) protein. In OCL mice, Pi excretion in feces was markedly reduced (P < 0.01). Together, these effects of osteocyte ablation are predicted to markedly increase intestinal Pi absorption (P < 0.01), thus suggesting that increased intestinal Pi absorption stimulates renal Pi excretion in OCL mice. In addition, the ablation of osteocytes and feeding of a high Pi diet affected FGF15/bile acid metabolism and controlled Npt2b expression. In conclusion, OCL mice exhibited increased renal Pi excretion due to enhanced intestinal Pi absorption. We discuss the role of FGF23-Klotho on renal and intestinal Pi metabolism in OCL mice.

DOI： 10.3389/fendo.2017.00359

Web of Science

PubMed

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