研究者詳細 - 辰巳佐和子

論文 - 辰巳佐和子

分割表示139 件中 101 - 120 件目／全件表示 >>

Regulation of intestinal Na+-dependent phosphate co-transporters by a low-phosphate diet and 1,25-dihydroxyvitamin D3. 国際誌

Katai K, Miyamoto K, Kishida S, Segawa H, Nii T, Tanaka H, Tani Y, Arai H, Tatsumi S, Morita K, Taketani Y, Takeda E.

Biochem J. 343 ( Pt 3 ) 705 - 712 1999年11月

Regulation of intestinal Na+-dependent phosphate co-transporters by a low-phosphate diet and 1,25-dihydroxyvitamin D3. 査読

Katai K, Miyamoto K, Kishida S, Segawa H, Nii T, Tanaka H, Tani Y, Arai H, Tatsumi S, Morita K, Taketani Y, Takeda E

The Biochemical journal 343 Pt 3 705 - 712 1999年11月

Nicotinamide inhibits sodium-dependent phosphate cotransport activity in rat small intestine. 国際誌

Katai K, Tanaka H, Tatsumi S, Fukunaga Y, Genjida K, Morita K, Kuboyama N, Suzuki T, Akiba T, Miyamoto K, Takeda E.

Nephrol Dial Transplant. 14 ( 5 ) 195 - 201 1999年5月

Regulation of PiT-1, a sodium-dependent phosphate co-transporter in rat parathyroid glands. 招待国際誌

Miyamoto K, Tatsumi S, Segawa H, Morita K, Nii T, Fujioka A, Kitano M, Inoue Y, Takeda E.

Nephrol Dial Transplant. 14 ( Suppl 1 ) 73 - 75 1999年5月

Nicotinamide inhibits sodium-dependent phosphate cotransport activity in rat small intestine 査読

K Katai, H Tanaka, S Tatsumi, Y Fukunaga, K Genjida, K Morita, N Kuboyama, T Suzuki, T Akiba, K Miyamoto, E Takeda

NEPHROLOGY DIALYSIS TRANSPLANTATION 14 ( 5 ) 1195 - 1201 1999年5月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：OXFORD UNIV PRESS

Background. We recently reported that the administration of niceritorol (a nicotinic acid derivative which improves lipid metabolism and peripheral circulation, and is used for the treatment of hyperlipidaemia and impaired peripheral circulation) to patients with hyperphosphataemia undergoing dialysis decreased the serum phosphate (Pi) concentration. We found that this was due to an acceleration of faecal Pi excretion by niceritrol.
Methods. Intestinal brush border membrane vesicles (BBMVs) were prepared from rat jejunum, and the Na+-dependent and Na+-independent Pi transport activities in these vesicles were measured. In addition, the functional Pi transporter from rat small intestine was injected in Xenopus oocytes, and the effect of nicotinamide on the levels of its expression were measured by northern blotting.
Results. The Na+-dependent component was significantly decreased in the BBMVs isolated from rats treated with nicotinamide, while the Na+-independent component was not changed. Kinetic studies demonstrated that the decreased activity was due to reduction of the V-max value and not an elevation of the K-m values. When poly(A)(+)RNA from rats treated with nicotinamide was microinjected into Xenopus oocytes, the Pi transport activity was significantly decreased compared with that in the control animals. In addition, there were no significant changes in Na/Pi cotransporters and activators, but the vitamin D receptor mRNA level was reduced to 80% of the control level.
Conclusions. These observations suggest that nicotinamide may regulate the expression of a major functional Na/Pi cotransporter in the rat small intestine.

Web of Science

Regulation of PiT-1, a sodium-dependent phosphate co-transporter in rat parathyroid glands 査読

K Miyamoto, S Tatsumi, H Segawa, K Morita, T Nii, A Fujioka, M Kitano, Y Inoue, E Takeda

NEPHROLOGY DIALYSIS TRANSPLANTATION 14 73 - 75 1999年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：OXFORD UNIV PRESS

A cDNA encoding an Na+-P-i co-transporter, termed rat PiT-1, has now been isolated from rat parathyroid. Expression of rat PiT-1 in Xenopus oocytes revealed that it possesses Na+-dependent P-i co-transport activity. The amount of PiT-1 mRNA in the parathyroid of vitamin D-deficient rats was reduced compared with that in normal animals, and increased markedly after administration of 1,25-dihydroxyvitamin D3, Furthermore, the abundance of PiT-1 mRNA in the parathyroid was much greater in rats fed a low-P-i diet than in those fed a high-P-i diet. Thus, rat PiT-1 may contribute to the effects of P-i and vitamin D on parathyroid function.

Web of Science

Does the parathyroid 'see' phosphate? 国際誌

Miyamoto K, Tatsumi S, Morita K, Takeda E.

Nephrol Dial Transplant. 13 ( 11 ) 2727 - 2729 1998年11月

Identification of three isoforms for the Na+-dependent phosphate cotransporter (NaPi-2) in rat kidney. 国際誌

Tatsumi S, Miyamoto K, Kouda T, Motonaga K, Katai K, Ohkido I, Morita K, Segawa H, Tani Y, Yamamoto H, Taketani Y, Takeda E.

J Biol Chem. 273 ( 44 ) 28568 - 28575 1998年10月

Identification of three isoforms for the Na+-dependent phosphate cotransporter (NaPi-2) in rat kidney 査読

S Tatsumi, K Miyamoto, T Kouda, K Motonaga, K Katai, Ohkido, I, K Morita, H Segawa, Y Tani, H Yamamoto, Y Taketani, E Takeda

JOURNAL OF BIOLOGICAL CHEMISTRY 273 ( 44 ) 28568 - 28575 1998年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

We have isolated three unique NaPi-2-related protein cDNAs (NaPi-2 alpha, NaPi-2 beta, and NaPi-2 gamma) from a rat kidney library. NaPi-2 alpha cDNA encodes 337 amino acids which have high homology to the N-terminal half of NaPi-2 containing 3 transmembrane domains. NaPi-2 beta encodes 327 amino acids which are identical to the N-terminal region of NaPi-2 containing 4 transmembrane domains, whereas the 146 amino acids in the C-terminal region are completely different. In contrast, NaPi-2 gamma encodes 268 amino acids which are identical to the C-terminal half of NaPi-2, An analysis of phage and cosmid clones indicated that the three related proteins were produced by alternative splicing in the NaPi-2 gene. In a rabbit reticulocyte lysate system, NaPi-2 alpha, beta, and gamma were found to be 36, 36, and 29 kDa amino acid polypeptides, respectively. NaPi-2 alpha: and NaPi-2 gamma were glycosylated and revealed to be 45- and 35-kDa proteins, respectively. In isolated brush-border membrane vesicles, an N-terminal antibody was reacted with 45- and 40-kDa, and a C-terminal antibody was reacted with 37-kDa protein. The sizes of these proteins corresponded to those in glycosylated forms.
A functional analysis demonstrated that NaPi-2 gamma and -2 alpha markedly inhibited NaPi-2 activity in Xenopus oocytes, The results suggest that these short isoforms may function as a dominant negative inhibitor of the full-length transporter.

Web of Science

Effects of dietary Pi on the renal Na+-dependent Pi transporter NaPi-2 in thyroparathyroidectomized rats. 国際誌

Takahashi F, Morita K, Katai K, Segawa H, Fujioka A, Kouda T, Tatsumi S, Nii T, Taketani Y, Haga H, Hisano S, Fukui Y, Miyamoto KI, Takeda E.

Biochem J. 333 ( Pt1 ) 175 - 181 1998年7月

Effects of dietary Pi on the renal Na+-dependent Pi transporter NaPi-2 in thyroparathyroidectomized rats. 査読

Takahashi F, Morita K, Katai K, Segawa H, Fujioka A, Kouda T, Tatsumi S, Nii T, Taketani Y, Haga H, Hisano S, Fukui Y, Miyamoto KI, Takeda E

The Biochemical journal 333 ( Pt 1) 175 - 181 1998年7月

Regulation of type II renal Na+-dependent inorganic phosphate transporters by 1,25-dihydroxyvitamin D3. Identification of a vitamin D-responsive element in the human NAPi-3 gene. 国際誌

Taketani Y, Segawa H, Chikamori M, Morita K, Tanaka K, Kido S, Yamamoto H, Iemori Y, Tatsumi S, Tsugawa N, Okano T, Kobayashi T, Miyamoto K, Takeda E.

J Biol Chem. 273 ( 23 ) 14575 - 14581 1998年6月

Regulation of type II renal Na+-dependent inorganic phosphate transporters by 1,25-dihydroxyvitamin D-3 - Identification of a vitamin D-responsive element in the human NAPI-3 gene 査読

Y Taketani, H Segawa, M Chikamori, K Morita, K Tanaka, S Kido, H Yamamoto, Y Iemori, S Tatsumi, N Tsugawa, T Okano, T Kobayashi, K Miyamoto, E Takeda

JOURNAL OF BIOLOGICAL CHEMISTRY 273 ( 23 ) 14575 - 14581 1998年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

Vitamin D is an important regulator of phosphate homeostasis. The effects of vitamin D on the expression of renal Na+-dependent inorganic phosphate (P-i) transporters (types I and II) were investigated. In vitamin D-deficient rats, the amounts of type II Na+-dependent P-i transporter (NaPi-2) protein and mRNA were decreased in the juxtamedullary kidney cortex, but not in the superficial cortex, compared with control rats. The administration of 1,25-dihydroxyvitamin D-3 (1,25-(OH)(2)D-3) to vitamin D-deficient rats increased the initial rate of P-i uptake as well as the amounts of NaPi-2 mRNA and protein in the juxtamedullary cortex. The transcriptional activity of a luciferase reporter plasmid containing the promoter region of the human type II Na+-dependent P-i transporter NaPi-3 gene was increased markedly by 1,25-(OH)(2)D-3 in COS-7 cells expressing the human vitamin D receptor. A deletion and mutation analysis of the NaPi-3 gene promoter identified the vitamin D-responsive element as the sequence 5'-GGGGCAGCAAGGGCA-3' nucleotides -1977 to -1963 relative to the transcription start site. This element bound a heterodimer of the vitamin D receptor and retinoid X receptor, and it enhanced the basal transcriptional activity of the promoter of the herpes simplex virus thymidine kinase gene in an orientation-independent manner. Thus, one mechanism by which vitamin D regulates P-i homeostasis is through the modulation of the expression of type II Na+-dependent P-i transporter genes in the juxtamedullary kidney cortex.

DOI： 10.1074/jbc.273.23.14575

Web of Science

Characterization of the 5' flanking region of the human NPT-1 Na+/phosphate cotransporter gene. 国際誌

Taketani Y, Miyamoto K, Chikamori M, Tanaka K, Yamamoto H, Tatsumi S, Morita K, Takeda E.

Biochim Biophys Acta. 1396 ( 3 ) 267 - 272 1998年5月

Molecular cloning and hormonal regulation of PiT-1, a sodium-dependent phosphate cotransporter from rat parathyroid glands. 国際誌

Tatsumi S, Segawa H, Morita K, Haga H, Kouda T, Yamamoto H, Inoue Y, Nii T, Katai K, Taketani Y, Miyamoto KI, Takeda E.

Endocrinology. 139 ( 4 ) 1692 - 1699 1998年4月

Molecular cloning and hormonal regulation of PiT-1, a sodium-dependent phosphate cotransporter from rat parathyroid glands. 査読

Tatsumi S, Segawa H, Morita K, Haga H, Kouda T, Yamamoto H, Inoue Y, Nii T, Katai K, Taketani Y, Miyamoto KI, Takeda E

Endocrinology 139 ( 4 ) 1692 - 1699 1998年4月

Characterization of the 5 ' flanking region of the human NPT-1 Na+/phosphate cotransporter gene 査読

Y Taketani, K Miyamoto, M Chikamori, K Tanaka, H Yamamoto, S Tatsumi, K Morita, E Takeda

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION 1396 ( 3 ) 267 - 272 1998年3月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE BV

To elucidate the expression and regulation of the human type I Na+/phosphate transporter gene (NPT-I), the 5' flanking region of the NPT-1 gene was cloned, and its nucleotide sequence and function were determined. A genomic clone that contained approximately 14.0 kb of the 5'-flanking region of the NPT-1 gene was isolated. A single transcription start site was located 104 base pairs (bp) upstream of the 3' end of exon 1. In addition to the sequence of the S-flanking region contained a sequence weakly homologous to a TATA box at position -41 to -36 and many transcriptional regulatory elements.' Transient expression revealed that a 45-bp region of proximal to exon 1, which contained TATA-like sequence, was sufficient for promoting luciferase expression in OK-cells derived from opossum kidney proximal tubule. (C) 1998 Elsevier Science B.V.

Web of Science

Characterization of the 5' flanking region of the human NPT-1 Na+/phosphate cotransporter gene. 査読

Taketani Y, Miyamoto K, Chikamori M, Tanaka K, Yamamoto H, Tatsumi S, Morita K, Takeda E

Biochimica et biophysica acta 1396 ( 3 ) 267 - 272 1998年3月

Cloning, functional expression and dietary regulation of the mouse neutral and basic amino acid transporter (NBAT). 査読

Segawa H, Miyamoto K, Ogura Y, Haga H, Morita K, Katai K, Tatsumi S, Nii T, Taketani Y, Takeda E

The Biochemical journal 328 ( Pt 2) 657 - 664 1997年12月

Relative contributions of Na+-dependent phosphate co-transporters to phosphate transport in mouse kidney: RNase H-mediated hybrid depletion analysis. 国際誌

Miyamoto K, Segawa H, Morita K, Nii T, Tatsumi S, Taketani Y, Takeda E.

Biochem J. 327 ( Pt3 ) 735 - 739 1997年11月

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