研究者詳細 - 辰巳佐和子

論文 - 辰巳佐和子

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Relative contributions of Na+-dependent phosphate co-transporters to phosphate transport in mouse kidney: RNase H-mediated hybrid depletion analysis. 査読

Miyamoto K, Segawa H, Morita K, Nii T, Tatsumi S, Taketani Y, Takeda E

The Biochemical journal 327 ( Pt 3) 735 - 739 1997年11月

Immunohistochemical and RT-PCR detection of Na+-dependent inorganic phosphate cotransporter (NaPi-2) in rat brain. 国際誌

Hisano S, Haga H, Li Z, Tatsumi S, Miyamoto KI, Takeda E, Fukui Y.

Brain Res. 772 ( 1, 2 ) 149 - 155 1997年10月

Immunohistochemical and RT-PCR detection of Na+-dependent inorganic phosphate cotransporter (NaP(i)-2) in rat brain 査読

Setsuji Hisano, Hiromi Haga, Zhunan Li, Sawako Tatsumi, Ken-Ichi Miyamoto, Eiji Takeda, Yoshihiro Fukui

Brain Research 772 ( 1-2 ) 149 - 155 1997年10月

記述言語：英語掲載種別：研究論文（学術雑誌）

Expression of a renal Na+-dependent inorganic phosphate (P(i)) cotransporter (NaP(i)-2) was studied in rat forebrain with reverse transcription and polymerase chain reaction (RT-PCR) and immunohistochemistry. RT-PCR analysis for total RNA from whole brain and sequencing of the PCR products showed expression of NaP(i)-2 mRNA in the brain. Immunohistochemical analysis revealed NaP(i)-2 staining in many nonpyramidal neurons of all six layers throughout neocortical areas and in neurons of proisocortical and periallocortical areas. NaP(i)-2-immunoreactive neurons were also detectable in the piriform cortex, hippocampal formation, caudate-putamen, amygdaloid nuclei and lateral geniculate nucleus. Furthermore, NaP(i)-2 staining was shown in ependymal cells and microvascular endothelial cells. The present results suggest that NaP(i)-2 is synthesized within the brain and involved in maintaining P(i) homeostasis of certain neurons and/or the entire brain.

DOI： 10.1016/S0006-8993(97)00884-6

Scopus

PubMed

Cloning, functional expression and dietary regulation of the mouse neutral and basic amino acid transporter (NBAT) 国際誌

Segawa H, Miyamoto K, Ogura Y, Haga H, Morita K, Katai K, Tatsumi S, Nii T, Taketani Y, Takeda E

Biochem J. 328 ( Pt 2 ) 657 - 664 1997年10月

Structural organization of the human vitamin D receptor chromosomal gene and its promoter. 国際誌

Miyamoto K, Kesterson RA, Yamamoto H, Taketani Y, Nishiwaki E, Tatsumi S, Inoue Y, Morita K, Takeda E, Pike JW.

Mol Endocrinol. 11 ( 8 ) 1165 - 1179 1997年7月

Gene structure and functional analysis of the human Na+/phosphate co-transporter 国際誌

Taketani Y, Miyamoto Ki, Tanaka K, Katai K, Chikamori M, Tatsumi S, Segawa H, Yamamoto H, Morita K, Takeda E.

Biochem J. 324 ( Pt 3 ) 927 - 934 1997年6月

Gene structure and functional analysis of the human Na+/phosphate co-transporter. 査読

Taketani Y, Miyamoto Ki, Tanaka K, Katai K, Chikamori M, Tatsumi S, Segawa H, Yamamoto H, Morita K, Takeda E

The Biochemical journal 324 ( Pt 3) 927 - 934 1997年6月

Acute regulation by dietary phosphate of the sodium-dependent phosphate transporter (NaP(i)-2) in rat kidney. 国際誌

Katai K, Segawa H, Haga H, Morita K, Arai H, Tatsumi S, Taketani Y, Miyamoto K, Hisano S, Fukui Y, Takeda E.

J Biochem. 121 ( 1 ) 50 - 55 1997年1月

Acute regulation by dietary phosphate of the sodium-dependent phosphate transporter (NaP(i)-2) in rat kidney. 査読

Katai K, Segawa H, Haga H, Morita K, Arai H, Tatsumi S, Taketani Y, Miyamoto K, Hisano S, Fukui Y, Takeda E

Journal of biochemistry 121 ( 1 ) 50 - 55 1997年1月

記述言語：英語出版者・発行元：The Japanese Biochemical Society

Alteration of the dietary intake of phosphate (Pi) leads to rapid changes in renal Pi transport activity. The present study, examined the underlying cellular mechanisms of the rapid regulation, with special reference to renal P, cotransporter. Rats were fed either a low-Pi (0. 02%) diet (CLP rats), the low-Pi diet followed by a high-P, (1. 2%) diet (AHP rats), or a normal (0. 6%) diet (control rats). Nat-dependent Pi transport activity in the brush border membrane was significantly increased in CLP rats compared with control rats, and this activity decreased rapidly within 2 h after the change of diet in AHP rats. Kinetic analysis of Pi transport in the AHP rats indicated that the reduction was accompanied by a decrease in the apparent Vmax, for Na+-dependent Pi uptake. Northern blot analysis showed no difference in the abundance of NaPi-2 mRNA of the kidney between AHP and CLP rats. In contrast, Western blot analysis of renal brush border membrane proteins of AHP rats indicated a significant decrease in the abundance of NaPi-2 protein as compared with CLP rats. Immunoreactive signals for NaPi-2 were detected in lysosomal fractions of AHP and CLP rats. Immunohistochemical analysis showed that, NaPi-2 immunoreactivity in AHP rats was largely reduced in the apical membrane of the proximal tubular epithelial cells. Neither cycloheximide nor actinomycin D affected high-Pi-induced reduction of NaPi-2 protein in the brush border membrane of AHP rats, indicating that de novo protein synthesis of an unidentified regulator protein was not involved in the mechanism of this reduction. In contrast, treatment with colchicine, which disrupts microtubulers, abolished the effect of high-Pi diet on NaP, -2 expression. These results suggested that rapid en-docytotic internalization of NaP, -2 may occur specificallyin the brush border membrane following an acute increase in dietary Pi intake.

PubMed

Effects of truncation of the COOH-terminal region of a Na+-independent neutral and basic amino acid transporter on amino acid transport in Xenopus oocytes. 国際誌

Miyamoto K, Segawa H, Tatsumi S, Katai K, Yamamoto H, Taketani Y, Haga H, Morita K, Takeda E.

J Biol Chem. 271 ( 28 ) 16758 - 16763 1996年7月

Role of rBAT gene products in cystinuria. 国際誌

Miyamoto K, Katai K, Tatsumi S, Sone K, Segawa H, Takada K, Yamamoto H, Taketani Y, Morita K, Kanayama H, Kagawa S, Takeda E.

Int J Urol. 3 ( 1 ) S92 - S94 1996年1月

Role of rBAT gene products in cystinuria 査読

Ken-ichi Miyamoto, Kanako Katai, Sawako Tatsumi, Kanako Sone, Hiroko Segawa, Kazumi Takada, Hironori Yamamoto, Yutaka Taketani, Kyoko Morita, Hiroomi Kanayama, Susumu Kagawa, Eiji Takeda

International Journal of Urology 3 ( 1 ) s92 - s94 1996年

記述言語：英語掲載種別：研究論文（学術雑誌）

To investigate whether rBAT gene products function as a crystine transporter component or as a transport activator, we microinjected several C-terminal deletion mutants of rBAT cRNA into Xenopus oocytes, and measured transport activity for arginine, leucine and cystine in the presence and absence of sodium. Wild type rBAT significantly stimulated the uptake of all 3 amino acids 10-20 fold compared to control mutants. On the other hand, no mutant, except a Δ511-685 mutant, stimulated the uptake of these amino acids. However, the Δ511-685 mutant significantly increased the uptake of arginine. In the presence of sodium, the Δ511-685 mutant also increased the uptake of leucine. The Δ511-685 mutant did not stimulate crystine uptake in the presence and absence of sodium. Furthermore, inhibition of L-arginine uptake by L-homoserine was seen only in the presence of sodium. These results suggest that mutant rBAT stimulates the endogenous amino acid transport system y+ in oocytes. Finally, rBAT gene products, as the primary cause of cystinuria, may function as activators of the amino acid transport system in renal brush border membrane. © IUA/CLJ.

DOI： 10.1111/j.1442-2042.1996.tb00354.x

Scopus

PubMed

Mutations of the basic amino acid transporter gene associated with cystinuria. 国際誌

Miyamoto K, Katai K, Tatsumi S, Sone K, Segawa H, Yamamoto H, Taketani Y, Takada K, Morita K, Kanayama H, et al.

Biochem J. 310 ( Pt 3 ) 951 - 955 1995年9月

Mutations of the basic amino acid transporter gene associated with cystinuria. 査読

Miyamoto K, Katai K, Tatsumi S, Sone K, Segawa H, Yamamoto H, Taketani Y, Takada K, Morita K, Kanayama H

The Biochemical journal 310 ( Pt 3) 951 - 955 1995年9月

Chromosome assignments of genes for human Na(+)-dependent phosphate co-transporters NaPi-3 and NPT-1. 査読

Miyamoto K, Tatsumi S, Yamamoto H, Katai K, Taketani Y, Morita K, Takeda E

The Tokushima journal of experimental medicine 42 ( 1-2 ) 5 - 9 1995年7月

Cloning and functional expression of a Na(+)-dependent phosphate co-transporter from human kidney: cDNA cloning and functional expression. 国際誌

Miyamoto K, Tatsumi S, Sonoda T, Yamamoto H, Minami H, Taketani Y, Takeda E.

Biochem J. 305 ( Pt 1 ) 81 - 85 1995年1月

Cloning and functional expression of a Na(+)-dependent phosphate co-transporter from human kidney: cDNA cloning and functional expression. 査読

Miyamoto K, Tatsumi S, Sonoda T, Yamamoto H, Minami H, Taketani Y, Takeda E

The Biochemical journal 305 ( Pt 1) 81 - 85 1995年1月

Characterization of the rabbit intestinal fructose transporter (GLUT5). 国際誌

Miyamoto K, Tatsumi S, Morimoto A, Minami H, Yamamoto H, Sone K, Taketani Y, Nakabou Y, Oka T, Takeda E.

Biochem J. 303 ( Pt 3 ) 877 - 883 1994年11月

Characterization of the rabbit intestinal fructose transporter (GLUT5). 査読

Miyamoto K, Tatsumi S, Morimoto A, Minami H, Yamamoto H, Sone K, Taketani Y, Nakabou Y, Oka T, Takeda E

The Biochemical journal 303 ( Pt 3) 877 - 883 1994年11月

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